Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS03120 and LRK54_RS03125 are separated by 111 nucleotides LRK54_RS03125 and LRK54_RS03130 are separated by 159 nucleotides LRK54_RS03130 and LRK54_RS03135 are separated by 96 nucleotides
LRK54_RS03120: LRK54_RS03120 - protein translocase subunit SecD, at 672,205 to 674,088
_RS03120
LRK54_RS03125: LRK54_RS03125 - preprotein translocase subunit YajC, at 674,200 to 674,532
_RS03125
LRK54_RS03130: LRK54_RS03130 - tRNA guanosine(34) transglycosylase Tgt, at 674,692 to 675,819
_RS03130
LRK54_RS03135: LRK54_RS03135 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 675,916 to 676,947
_RS03135
Position (kb) 674
675
676 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.427 kb on - strand, within LRK54_RS03125 at 674.551 kb on - strand at 674.551 kb on - strand at 674.551 kb on - strand at 674.551 kb on - strand at 674.551 kb on - strand at 674.601 kb on - strand at 674.601 kb on - strand at 674.601 kb on - strand at 674.601 kb on - strand at 674.601 kb on - strand at 674.693 kb on + strand at 674.694 kb on - strand at 674.694 kb on - strand at 674.694 kb on - strand at 674.694 kb on - strand at 674.694 kb on - strand at 674.694 kb on - strand at 674.723 kb on + strand at 674.723 kb on + strand at 674.723 kb on + strand at 674.723 kb on + strand at 674.723 kb on + strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.724 kb on - strand at 674.795 kb on + strand at 674.795 kb on + strand at 674.795 kb on + strand at 674.795 kb on + strand at 674.795 kb on + strand at 674.795 kb on + strand at 674.795 kb on + strand at 674.795 kb on + strand at 674.795 kb on + strand at 674.796 kb on - strand at 674.796 kb on - strand at 674.796 kb on - strand at 674.796 kb on - strand at 674.796 kb on - strand at 674.796 kb on - strand at 674.796 kb on - strand at 674.796 kb on - strand at 674.796 kb on - strand at 674.798 kb on + strand at 674.798 kb on + strand at 674.798 kb on + strand at 674.798 kb on + strand at 674.798 kb on + strand at 674.798 kb on + strand at 674.867 kb on + strand, within LRK54_RS03130 at 674.867 kb on + strand, within LRK54_RS03130 at 674.867 kb on + strand, within LRK54_RS03130 at 674.867 kb on + strand, within LRK54_RS03130 at 674.867 kb on + strand, within LRK54_RS03130 at 674.868 kb on - strand, within LRK54_RS03130 at 674.868 kb on - strand, within LRK54_RS03130 at 674.868 kb on - strand, within LRK54_RS03130 at 674.868 kb on - strand, within LRK54_RS03130 at 674.868 kb on - strand, within LRK54_RS03130 at 674.868 kb on - strand, within LRK54_RS03130 at 674.897 kb on + strand, within LRK54_RS03130 at 674.897 kb on + strand, within LRK54_RS03130 at 674.897 kb on + strand, within LRK54_RS03130 at 674.898 kb on - strand, within LRK54_RS03130 at 674.898 kb on - strand, within LRK54_RS03130 at 674.898 kb on - strand, within LRK54_RS03130 at 674.898 kb on - strand, within LRK54_RS03130 at 674.898 kb on - strand, within LRK54_RS03130 at 674.898 kb on - strand, within LRK54_RS03130 at 674.898 kb on - strand, within LRK54_RS03130 at 674.898 kb on - strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.965 kb on - strand, within LRK54_RS03130 at 675.006 kb on + strand, within LRK54_RS03130 at 675.006 kb on + strand, within LRK54_RS03130 at 675.007 kb on - strand, within LRK54_RS03130 at 675.007 kb on - strand, within LRK54_RS03130 at 675.086 kb on + strand, within LRK54_RS03130 at 675.182 kb on + strand, within LRK54_RS03130 at 675.183 kb on - strand, within LRK54_RS03130 at 675.239 kb on + strand, within LRK54_RS03130 at 675.239 kb on + strand, within LRK54_RS03130 at 675.240 kb on - strand, within LRK54_RS03130 at 675.240 kb on - strand, within LRK54_RS03130 at 675.369 kb on - strand, within LRK54_RS03130 at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.992 kb on + strand at 675.992 kb on + strand at 675.992 kb on + strand at 675.992 kb on + strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 676.350 kb on - strand, within LRK54_RS03135 at 676.350 kb on - strand, within LRK54_RS03135 at 676.350 kb on - strand, within LRK54_RS03135 at 676.417 kb on - strand, within LRK54_RS03135 at 676.460 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.743 kb on - strand, within LRK54_RS03135
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 674,427 - LRK54_RS03125 0.68 +0.7 674,427 - LRK54_RS03125 0.68 -1.0 674,427 - LRK54_RS03125 0.68 -1.3 674,427 - LRK54_RS03125 0.68 -1.4 674,427 - LRK54_RS03125 0.68 -1.3 674,427 - LRK54_RS03125 0.68 -0.0 674,427 - LRK54_RS03125 0.68 +0.3 674,427 - LRK54_RS03125 0.68 -0.3 674,427 - LRK54_RS03125 0.68 +2.3 674,427 - LRK54_RS03125 0.68 -0.3 674,551 - -0.6 674,551 - -0.2 674,551 - -0.5 674,551 - -0.5 674,551 - +0.4 674,601 - -0.2 674,601 - -0.3 674,601 - -0.1 674,601 - -0.6 674,601 - +0.6 674,693 + -0.6 674,694 - +0.8 674,694 - +0.6 674,694 - -0.1 674,694 - +1.3 674,694 - +0.3 674,694 - +0.3 674,723 + +0.1 674,723 + -0.3 674,723 + -0.0 674,723 + -0.9 674,723 + +0.4 674,724 - -1.1 674,724 - +1.0 674,724 - +0.1 674,724 - -1.8 674,724 - -0.6 674,724 - +1.3 674,724 - -0.3 674,724 - +0.7 674,724 - -0.5 674,724 - +0.9 674,724 - -1.4 674,724 - -1.2 674,795 + +2.3 674,795 + -0.6 674,795 + -0.9 674,795 + -0.9 674,795 + -0.9 674,795 + -0.0 674,795 + +1.6 674,795 + -2.0 674,795 + +0.3 674,796 - +0.5 674,796 - -0.2 674,796 - -0.9 674,796 - -0.2 674,796 - -0.0 674,796 - -0.8 674,796 - +0.3 674,796 - -1.0 674,796 - +0.3 674,798 + -2.2 674,798 + +1.0 674,798 + -0.8 674,798 + -0.2 674,798 + +0.8 674,798 + -1.5 674,867 + LRK54_RS03130 0.16 -0.5 674,867 + LRK54_RS03130 0.16 -1.4 674,867 + LRK54_RS03130 0.16 +0.1 674,867 + LRK54_RS03130 0.16 +0.3 674,867 + LRK54_RS03130 0.16 -1.0 674,868 - LRK54_RS03130 0.16 -0.2 674,868 - LRK54_RS03130 0.16 -0.9 674,868 - LRK54_RS03130 0.16 -1.2 674,868 - LRK54_RS03130 0.16 -0.1 674,868 - LRK54_RS03130 0.16 +0.6 674,868 - LRK54_RS03130 0.16 -1.9 674,897 + LRK54_RS03130 0.18 -0.7 674,897 + LRK54_RS03130 0.18 +0.6 674,897 + LRK54_RS03130 0.18 +0.7 674,898 - LRK54_RS03130 0.18 -0.5 674,898 - LRK54_RS03130 0.18 -1.4 674,898 - LRK54_RS03130 0.18 -3.2 674,898 - LRK54_RS03130 0.18 -0.7 674,898 - LRK54_RS03130 0.18 +0.2 674,898 - LRK54_RS03130 0.18 +2.0 674,898 - LRK54_RS03130 0.18 -1.1 674,898 - LRK54_RS03130 0.18 -0.0 674,939 + LRK54_RS03130 0.22 -1.8 674,939 + LRK54_RS03130 0.22 +0.6 674,939 + LRK54_RS03130 0.22 -0.8 674,939 + LRK54_RS03130 0.22 -2.9 674,939 + LRK54_RS03130 0.22 +0.3 674,939 + LRK54_RS03130 0.22 -0.9 674,939 + LRK54_RS03130 0.22 -0.8 674,939 + LRK54_RS03130 0.22 -0.6 674,939 + LRK54_RS03130 0.22 -0.1 674,940 - LRK54_RS03130 0.22 -0.3 674,940 - LRK54_RS03130 0.22 +0.5 674,940 - LRK54_RS03130 0.22 -0.5 674,940 - LRK54_RS03130 0.22 -1.2 674,940 - LRK54_RS03130 0.22 +0.7 674,940 - LRK54_RS03130 0.22 -1.1 674,940 - LRK54_RS03130 0.22 -0.7 674,940 - LRK54_RS03130 0.22 +0.7 674,965 - LRK54_RS03130 0.24 -1.2 675,006 + LRK54_RS03130 0.28 -0.0 675,006 + LRK54_RS03130 0.28 +1.3 675,007 - LRK54_RS03130 0.28 -0.8 675,007 - LRK54_RS03130 0.28 -1.3 675,086 + LRK54_RS03130 0.35 +1.3 675,182 + LRK54_RS03130 0.43 -1.4 675,183 - LRK54_RS03130 0.44 +1.3 675,239 + LRK54_RS03130 0.48 -2.8 675,239 + LRK54_RS03130 0.48 -0.1 675,240 - LRK54_RS03130 0.49 -0.2 675,240 - LRK54_RS03130 0.49 -0.5 675,369 - LRK54_RS03130 0.60 -0.9 675,707 + -0.7 675,707 + -1.2 675,707 + -0.8 675,707 + -2.0 675,707 + -0.8 675,707 + +0.4 675,707 + +0.1 675,707 + -0.2 675,707 + -0.7 675,707 + +0.5 675,707 + -2.2 675,708 - -1.7 675,708 - +0.5 675,708 - -1.4 675,708 - +1.3 675,708 - -0.0 675,708 - +0.3 675,708 - +1.3 675,708 - +0.0 675,708 - -1.3 675,708 - +0.4 675,708 - +1.3 675,953 + +0.6 675,953 + -0.9 675,953 + -0.3 675,953 + +0.3 675,953 + +0.3 675,953 + +0.5 675,954 - -0.2 675,954 - -0.4 675,954 - +0.4 675,954 - +0.3 675,954 - +0.7 675,954 - +1.0 675,992 + -0.7 675,992 + +0.7 675,992 + +0.2 675,992 + -0.7 675,993 - +0.2 675,993 - +0.4 675,993 - +0.7 675,993 - -1.1 675,993 - -0.1 675,993 - -1.7 675,993 - +0.5 676,350 - LRK54_RS03135 0.42 +0.5 676,350 - LRK54_RS03135 0.42 -0.5 676,350 - LRK54_RS03135 0.42 -0.4 676,417 - LRK54_RS03135 0.49 -0.0 676,460 + LRK54_RS03135 0.53 -0.7 676,499 + LRK54_RS03135 0.56 -0.7 676,499 + LRK54_RS03135 0.56 +0.2 676,499 + LRK54_RS03135 0.56 -0.6 676,499 + LRK54_RS03135 0.56 -1.5 676,499 + LRK54_RS03135 0.56 -0.5 676,499 + LRK54_RS03135 0.56 +0.2 676,499 + LRK54_RS03135 0.56 -0.5 676,500 - LRK54_RS03135 0.57 -0.6 676,500 - LRK54_RS03135 0.57 +0.1 676,500 - LRK54_RS03135 0.57 +0.4 676,500 - LRK54_RS03135 0.57 -0.9 676,500 - LRK54_RS03135 0.57 -0.2 676,500 - LRK54_RS03135 0.57 +0.3 676,500 - LRK54_RS03135 0.57 -0.2 676,500 - LRK54_RS03135 0.57 -0.5 676,500 - LRK54_RS03135 0.57 -0.2 676,500 - LRK54_RS03135 0.57 -0.9 676,500 - LRK54_RS03135 0.57 +0.4 676,500 - LRK54_RS03135 0.57 -0.9 676,500 - LRK54_RS03135 0.57 -1.4 676,742 + LRK54_RS03135 0.80 +0.5 676,742 + LRK54_RS03135 0.80 -0.9 676,742 + LRK54_RS03135 0.80 +1.3 676,742 + LRK54_RS03135 0.80 -0.6 676,742 + LRK54_RS03135 0.80 +0.6 676,742 + LRK54_RS03135 0.80 -1.6 676,743 - LRK54_RS03135 0.80 -1.0
Or see this region's nucleotide sequence