Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01723 and CCNA_01724 are separated by 152 nucleotides CCNA_01724 and CCNA_01725 overlap by 4 nucleotides CCNA_01725 and CCNA_01726 are separated by 125 nucleotides
CCNA_01723: CCNA_01723 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,849,599 to 1,850,426
_01723
CCNA_01724: CCNA_01724 - hypothetical protein, at 1,850,579 to 1,851,331
_01724
CCNA_01725: CCNA_01725 - beta-barrel assembly machine (BAM) protein BamB, at 1,851,328 to 1,852,758
_01725
CCNA_01726: CCNA_01726 - EngA-subfamily GTPase protein, at 1,852,884 to 1,854,650
_01726
Position (kb)
1851
1852
1853 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1850.421 kb on + strand at 1850.454 kb on + strand at 1850.454 kb on + strand at 1850.456 kb on + strand at 1850.462 kb on - strand at 1850.503 kb on + strand at 1850.526 kb on + strand at 1850.569 kb on + strand at 1850.623 kb on + strand at 1850.653 kb on + strand at 1850.743 kb on + strand, within CCNA_01724 at 1850.812 kb on + strand, within CCNA_01724 at 1850.974 kb on + strand, within CCNA_01724 at 1851.244 kb on - strand, within CCNA_01724 at 1851.244 kb on - strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724 at 1851.250 kb on + strand, within CCNA_01724 at 1852.863 kb on + strand at 1852.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 1,850,421 + +1.8 1,850,454 + +1.0 1,850,454 + +1.5 1,850,456 + +1.3 1,850,462 - -0.5 1,850,503 + -0.6 1,850,526 + -1.8 1,850,569 + -1.8 1,850,623 + -3.5 1,850,653 + -1.5 1,850,743 + CCNA_01724 0.22 -1.4 1,850,812 + CCNA_01724 0.31 -1.9 1,850,974 + CCNA_01724 0.52 +1.8 1,851,244 - CCNA_01724 0.88 -1.4 1,851,244 - CCNA_01724 0.88 -2.6 1,851,250 + CCNA_01724 0.89 -1.3 1,851,250 + CCNA_01724 0.89 -0.5 1,851,250 + CCNA_01724 0.89 -1.0 1,852,863 + +2.1 1,852,888 - +0.6
Or see this region's nucleotide sequence