Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18925 and CA265_RS18930 are separated by 87 nucleotides CA265_RS18930 and CA265_RS18935 are separated by 161 nucleotides CA265_RS18935 and CA265_RS18940 are separated by 39 nucleotides
CA265_RS18925: CA265_RS18925 - metallophosphoesterase, at 4,702,443 to 4,703,648
_RS18925
CA265_RS18930: CA265_RS18930 - N-acetylglucosamine-6-phosphate deacetylase, at 4,703,736 to 4,704,827
_RS18930
CA265_RS18935: CA265_RS18935 - UMP kinase, at 4,704,989 to 4,705,693
_RS18935
CA265_RS18940: CA265_RS18940 - ribosome recycling factor, at 4,705,733 to 4,706,296
_RS18940
Position (kb)
4703
4704
4705 Strain fitness (log2 ratio)
-2
-1
0
1 at 4702.741 kb on - strand, within CA265_RS18925 at 4702.819 kb on + strand, within CA265_RS18925 at 4702.820 kb on - strand, within CA265_RS18925 at 4702.838 kb on + strand, within CA265_RS18925 at 4702.847 kb on - strand, within CA265_RS18925 at 4702.889 kb on + strand, within CA265_RS18925 at 4702.937 kb on + strand, within CA265_RS18925 at 4702.939 kb on + strand, within CA265_RS18925 at 4702.940 kb on - strand, within CA265_RS18925 at 4702.942 kb on + strand, within CA265_RS18925 at 4702.943 kb on - strand, within CA265_RS18925 at 4702.996 kb on + strand, within CA265_RS18925 at 4702.997 kb on - strand, within CA265_RS18925 at 4703.046 kb on - strand, within CA265_RS18925 at 4703.085 kb on + strand, within CA265_RS18925 at 4703.086 kb on - strand, within CA265_RS18925 at 4703.171 kb on + strand, within CA265_RS18925 at 4703.258 kb on - strand, within CA265_RS18925 at 4703.301 kb on + strand, within CA265_RS18925 at 4703.307 kb on - strand, within CA265_RS18925 at 4703.309 kb on - strand, within CA265_RS18925 at 4703.311 kb on + strand, within CA265_RS18925 at 4703.346 kb on + strand, within CA265_RS18925 at 4703.460 kb on - strand, within CA265_RS18925 at 4703.492 kb on + strand, within CA265_RS18925 at 4703.580 kb on + strand at 4703.595 kb on + strand at 4703.628 kb on - strand at 4703.628 kb on - strand at 4703.682 kb on - strand at 4703.686 kb on - strand at 4703.686 kb on - strand at 4703.746 kb on + strand at 4703.756 kb on + strand at 4703.756 kb on + strand at 4703.804 kb on - strand at 4703.828 kb on + strand at 4703.996 kb on - strand, within CA265_RS18930 at 4704.030 kb on - strand, within CA265_RS18930 at 4704.039 kb on - strand, within CA265_RS18930 at 4704.202 kb on + strand, within CA265_RS18930 at 4704.203 kb on - strand, within CA265_RS18930 at 4704.206 kb on + strand, within CA265_RS18930 at 4704.251 kb on - strand, within CA265_RS18930 at 4704.251 kb on - strand, within CA265_RS18930 at 4704.260 kb on - strand, within CA265_RS18930 at 4704.294 kb on - strand, within CA265_RS18930 at 4704.296 kb on + strand, within CA265_RS18930 at 4704.296 kb on + strand, within CA265_RS18930 at 4704.327 kb on + strand, within CA265_RS18930 at 4704.327 kb on + strand, within CA265_RS18930 at 4704.328 kb on - strand, within CA265_RS18930 at 4704.416 kb on + strand, within CA265_RS18930 at 4704.426 kb on - strand, within CA265_RS18930 at 4704.463 kb on + strand, within CA265_RS18930 at 4704.464 kb on - strand, within CA265_RS18930 at 4704.479 kb on + strand, within CA265_RS18930 at 4704.480 kb on - strand, within CA265_RS18930 at 4704.545 kb on + strand, within CA265_RS18930 at 4704.582 kb on + strand, within CA265_RS18930 at 4704.583 kb on - strand, within CA265_RS18930 at 4704.583 kb on - strand, within CA265_RS18930 at 4704.620 kb on - strand, within CA265_RS18930 at 4704.629 kb on - strand, within CA265_RS18930 at 4704.741 kb on - strand at 4704.743 kb on - strand at 4704.802 kb on + strand at 4704.826 kb on - strand at 4704.878 kb on - strand at 4704.878 kb on - strand at 4704.955 kb on + strand at 4705.722 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,702,741 - CA265_RS18925 0.25 +0.3 4,702,819 + CA265_RS18925 0.31 +0.1 4,702,820 - CA265_RS18925 0.31 -1.1 4,702,838 + CA265_RS18925 0.33 +0.0 4,702,847 - CA265_RS18925 0.33 +0.8 4,702,889 + CA265_RS18925 0.37 +0.4 4,702,937 + CA265_RS18925 0.41 +0.7 4,702,939 + CA265_RS18925 0.41 +0.1 4,702,940 - CA265_RS18925 0.41 -0.7 4,702,942 + CA265_RS18925 0.41 +0.8 4,702,943 - CA265_RS18925 0.41 +0.4 4,702,996 + CA265_RS18925 0.46 +0.3 4,702,997 - CA265_RS18925 0.46 +1.4 4,703,046 - CA265_RS18925 0.50 -0.2 4,703,085 + CA265_RS18925 0.53 +1.4 4,703,086 - CA265_RS18925 0.53 +0.0 4,703,171 + CA265_RS18925 0.60 +0.1 4,703,258 - CA265_RS18925 0.68 +0.8 4,703,301 + CA265_RS18925 0.71 +0.3 4,703,307 - CA265_RS18925 0.72 +0.8 4,703,309 - CA265_RS18925 0.72 -0.0 4,703,311 + CA265_RS18925 0.72 +1.3 4,703,346 + CA265_RS18925 0.75 +0.2 4,703,460 - CA265_RS18925 0.84 +0.1 4,703,492 + CA265_RS18925 0.87 +0.9 4,703,580 + +0.2 4,703,595 + -2.7 4,703,628 - +0.3 4,703,628 - +0.8 4,703,682 - -0.8 4,703,686 - -1.0 4,703,686 - +0.9 4,703,746 + -0.3 4,703,756 + +1.2 4,703,756 + -0.4 4,703,804 - -0.1 4,703,828 + -1.9 4,703,996 - CA265_RS18930 0.24 -0.5 4,704,030 - CA265_RS18930 0.27 +0.3 4,704,039 - CA265_RS18930 0.28 +0.3 4,704,202 + CA265_RS18930 0.43 -1.3 4,704,203 - CA265_RS18930 0.43 +0.2 4,704,206 + CA265_RS18930 0.43 +0.2 4,704,251 - CA265_RS18930 0.47 +0.3 4,704,251 - CA265_RS18930 0.47 +1.1 4,704,260 - CA265_RS18930 0.48 +0.9 4,704,294 - CA265_RS18930 0.51 -0.4 4,704,296 + CA265_RS18930 0.51 -1.1 4,704,296 + CA265_RS18930 0.51 -1.0 4,704,327 + CA265_RS18930 0.54 +0.4 4,704,327 + CA265_RS18930 0.54 -1.3 4,704,328 - CA265_RS18930 0.54 -2.0 4,704,416 + CA265_RS18930 0.62 -0.7 4,704,426 - CA265_RS18930 0.63 -0.7 4,704,463 + CA265_RS18930 0.67 -1.0 4,704,464 - CA265_RS18930 0.67 -0.2 4,704,479 + CA265_RS18930 0.68 -1.0 4,704,480 - CA265_RS18930 0.68 +0.9 4,704,545 + CA265_RS18930 0.74 +0.9 4,704,582 + CA265_RS18930 0.77 -0.3 4,704,583 - CA265_RS18930 0.78 -0.5 4,704,583 - CA265_RS18930 0.78 +0.8 4,704,620 - CA265_RS18930 0.81 -0.2 4,704,629 - CA265_RS18930 0.82 -1.1 4,704,741 - -0.1 4,704,743 - -0.0 4,704,802 + -0.1 4,704,826 - -1.1 4,704,878 - +0.7 4,704,878 - -0.2 4,704,955 + +0.1 4,705,722 + -0.9
Or see this region's nucleotide sequence