Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04590 and CA265_RS04595 are separated by 282 nucleotides CA265_RS04595 and CA265_RS04600 are separated by 37 nucleotides CA265_RS04600 and CA265_RS04605 are separated by 26 nucleotides
CA265_RS04590: CA265_RS04590 - LysR family transcriptional regulator, at 1,097,366 to 1,098,232
_RS04590
CA265_RS04595: CA265_RS04595 - hypothetical protein, at 1,098,515 to 1,098,895
_RS04595
CA265_RS04600: CA265_RS04600 - hypothetical protein, at 1,098,933 to 1,099,772
_RS04600
CA265_RS04605: CA265_RS04605 - hydrolase TatD, at 1,099,799 to 1,100,785
_RS04605
Position (kb)
1098
1099 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1097.515 kb on + strand, within CA265_RS04590 at 1097.519 kb on - strand, within CA265_RS04590 at 1097.525 kb on - strand, within CA265_RS04590 at 1097.594 kb on + strand, within CA265_RS04590 at 1097.595 kb on - strand, within CA265_RS04590 at 1097.595 kb on - strand, within CA265_RS04590 at 1097.597 kb on - strand, within CA265_RS04590 at 1097.597 kb on - strand, within CA265_RS04590 at 1097.625 kb on + strand, within CA265_RS04590 at 1097.645 kb on - strand, within CA265_RS04590 at 1097.690 kb on - strand, within CA265_RS04590 at 1097.704 kb on + strand, within CA265_RS04590 at 1097.749 kb on - strand, within CA265_RS04590 at 1097.810 kb on - strand, within CA265_RS04590 at 1097.890 kb on + strand, within CA265_RS04590 at 1097.966 kb on - strand, within CA265_RS04590 at 1097.966 kb on - strand, within CA265_RS04590 at 1098.022 kb on - strand, within CA265_RS04590 at 1098.101 kb on + strand, within CA265_RS04590 at 1098.102 kb on - strand, within CA265_RS04590 at 1098.102 kb on - strand, within CA265_RS04590 at 1098.157 kb on + strand at 1098.158 kb on - strand at 1098.159 kb on + strand at 1098.253 kb on - strand at 1098.294 kb on + strand at 1098.396 kb on + strand at 1098.397 kb on - strand at 1098.397 kb on - strand at 1098.405 kb on - strand at 1098.406 kb on + strand at 1098.409 kb on - strand at 1098.606 kb on + strand, within CA265_RS04595 at 1098.610 kb on + strand, within CA265_RS04595 at 1098.611 kb on - strand, within CA265_RS04595 at 1098.619 kb on - strand, within CA265_RS04595 at 1098.674 kb on + strand, within CA265_RS04595 at 1098.685 kb on - strand, within CA265_RS04595 at 1098.696 kb on - strand, within CA265_RS04595 at 1098.704 kb on + strand, within CA265_RS04595 at 1098.739 kb on - strand, within CA265_RS04595 at 1098.759 kb on - strand, within CA265_RS04595 at 1098.914 kb on + strand at 1098.930 kb on - strand at 1098.930 kb on - strand at 1099.034 kb on + strand, within CA265_RS04600 at 1099.035 kb on - strand, within CA265_RS04600 at 1099.138 kb on - strand, within CA265_RS04600 at 1099.138 kb on - strand, within CA265_RS04600 at 1099.139 kb on + strand, within CA265_RS04600 at 1099.145 kb on + strand, within CA265_RS04600 at 1099.216 kb on + strand, within CA265_RS04600 at 1099.273 kb on + strand, within CA265_RS04600 at 1099.274 kb on - strand, within CA265_RS04600 at 1099.275 kb on + strand, within CA265_RS04600 at 1099.275 kb on + strand, within CA265_RS04600 at 1099.343 kb on + strand, within CA265_RS04600 at 1099.346 kb on + strand, within CA265_RS04600 at 1099.458 kb on - strand, within CA265_RS04600 at 1099.477 kb on + strand, within CA265_RS04600 at 1099.516 kb on + strand, within CA265_RS04600 at 1099.616 kb on + strand, within CA265_RS04600 at 1099.636 kb on - strand, within CA265_RS04600 at 1099.687 kb on - strand, within CA265_RS04600 at 1099.705 kb on - strand at 1099.706 kb on + strand at 1099.707 kb on - strand at 1099.777 kb on + strand at 1099.796 kb on + strand at 1099.798 kb on + strand at 1099.828 kb on + strand at 1099.828 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 1,097,515 + CA265_RS04590 0.17 +1.6 1,097,519 - CA265_RS04590 0.18 +0.5 1,097,525 - CA265_RS04590 0.18 -1.6 1,097,594 + CA265_RS04590 0.26 -0.0 1,097,595 - CA265_RS04590 0.26 +0.1 1,097,595 - CA265_RS04590 0.26 -0.6 1,097,597 - CA265_RS04590 0.27 -0.2 1,097,597 - CA265_RS04590 0.27 -1.2 1,097,625 + CA265_RS04590 0.30 -0.5 1,097,645 - CA265_RS04590 0.32 +0.2 1,097,690 - CA265_RS04590 0.37 -0.3 1,097,704 + CA265_RS04590 0.39 -0.4 1,097,749 - CA265_RS04590 0.44 -0.5 1,097,810 - CA265_RS04590 0.51 -0.2 1,097,890 + CA265_RS04590 0.60 +0.2 1,097,966 - CA265_RS04590 0.69 -0.7 1,097,966 - CA265_RS04590 0.69 -0.2 1,098,022 - CA265_RS04590 0.76 +0.2 1,098,101 + CA265_RS04590 0.85 -0.5 1,098,102 - CA265_RS04590 0.85 -0.4 1,098,102 - CA265_RS04590 0.85 +0.0 1,098,157 + -0.3 1,098,158 - -0.3 1,098,159 + +0.5 1,098,253 - +0.4 1,098,294 + +0.8 1,098,396 + +0.2 1,098,397 - +0.4 1,098,397 - -2.4 1,098,405 - -0.5 1,098,406 + -0.0 1,098,409 - +0.0 1,098,606 + CA265_RS04595 0.24 +0.7 1,098,610 + CA265_RS04595 0.25 +1.2 1,098,611 - CA265_RS04595 0.25 -0.6 1,098,619 - CA265_RS04595 0.27 +0.1 1,098,674 + CA265_RS04595 0.42 -0.4 1,098,685 - CA265_RS04595 0.45 -1.0 1,098,696 - CA265_RS04595 0.48 +0.2 1,098,704 + CA265_RS04595 0.50 -0.4 1,098,739 - CA265_RS04595 0.59 -0.0 1,098,759 - CA265_RS04595 0.64 -0.1 1,098,914 + -0.0 1,098,930 - -0.6 1,098,930 - -0.6 1,099,034 + CA265_RS04600 0.12 +0.9 1,099,035 - CA265_RS04600 0.12 +0.6 1,099,138 - CA265_RS04600 0.24 -1.7 1,099,138 - CA265_RS04600 0.24 +0.6 1,099,139 + CA265_RS04600 0.25 -2.0 1,099,145 + CA265_RS04600 0.25 +0.8 1,099,216 + CA265_RS04600 0.34 -1.3 1,099,273 + CA265_RS04600 0.40 -1.3 1,099,274 - CA265_RS04600 0.41 -1.1 1,099,275 + CA265_RS04600 0.41 +0.6 1,099,275 + CA265_RS04600 0.41 -0.4 1,099,343 + CA265_RS04600 0.49 -0.6 1,099,346 + CA265_RS04600 0.49 +0.0 1,099,458 - CA265_RS04600 0.62 -0.3 1,099,477 + CA265_RS04600 0.65 +0.3 1,099,516 + CA265_RS04600 0.69 +3.2 1,099,616 + CA265_RS04600 0.81 -0.2 1,099,636 - CA265_RS04600 0.84 +0.2 1,099,687 - CA265_RS04600 0.90 +0.6 1,099,705 - +0.6 1,099,706 + -0.1 1,099,707 - -0.2 1,099,777 + +1.2 1,099,796 + +0.4 1,099,798 + -0.1 1,099,828 + -0.3 1,099,828 + -0.2
Or see this region's nucleotide sequence