Experiment: MoYLS4 with Gentamicin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frdB and DvMF_2461 are separated by 306 nucleotides DvMF_2461 and DvMF_2462 are separated by 314 nucleotides DvMF_2462 and DvMF_2463 are separated by 44 nucleotides
DvMF_2460: frdB - fumarate reductase iron-sulfur subunit (RefSeq), at 3,103,544 to 3,104,287
frdB
DvMF_2461: DvMF_2461 - fumarate hydratase (RefSeq), at 3,104,594 to 3,105,433
_2461
DvMF_2462: DvMF_2462 - hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit (RefSeq), at 3,105,748 to 3,106,275
_2462
DvMF_2463: DvMF_2463 - hypothetical protein (RefSeq), at 3,106,320 to 3,107,615
_2463
Position (kb)
3104
3105
3106 Strain fitness (log2 ratio)
-1
0
1 at 3103.679 kb on + strand, within frdB at 3103.819 kb on - strand, within frdB at 3104.071 kb on - strand, within frdB at 3104.241 kb on + strand at 3104.249 kb on - strand at 3104.435 kb on + strand at 3104.856 kb on - strand, within DvMF_2461 at 3104.944 kb on + strand, within DvMF_2461 at 3105.343 kb on - strand, within DvMF_2461 at 3105.348 kb on + strand, within DvMF_2461 at 3105.555 kb on + strand at 3105.767 kb on + strand at 3105.834 kb on + strand, within DvMF_2462 at 3105.834 kb on + strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.868 kb on - strand, within DvMF_2462 at 3105.902 kb on + strand, within DvMF_2462 at 3105.972 kb on - strand, within DvMF_2462 at 3106.013 kb on - strand, within DvMF_2462 at 3106.085 kb on + strand, within DvMF_2462 at 3106.093 kb on - strand, within DvMF_2462 at 3106.311 kb on + strand at 3106.336 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.03 mg/ml remove 3,103,679 + frdB DvMF_2460 0.18 -0.8 3,103,819 - frdB DvMF_2460 0.37 -0.8 3,104,071 - frdB DvMF_2460 0.71 -0.9 3,104,241 + -0.8 3,104,249 - -0.8 3,104,435 + -0.7 3,104,856 - DvMF_2461 0.31 -0.4 3,104,944 + DvMF_2461 0.42 +0.0 3,105,343 - DvMF_2461 0.89 -0.4 3,105,348 + DvMF_2461 0.90 +0.1 3,105,555 + -0.2 3,105,767 + -0.6 3,105,834 + DvMF_2462 0.16 -0.2 3,105,834 + DvMF_2462 0.16 -0.1 3,105,842 - DvMF_2462 0.18 -0.7 3,105,842 - DvMF_2462 0.18 -0.4 3,105,842 - DvMF_2462 0.18 -0.3 3,105,868 - DvMF_2462 0.23 -0.3 3,105,902 + DvMF_2462 0.29 -0.3 3,105,972 - DvMF_2462 0.42 -0.2 3,106,013 - DvMF_2462 0.50 -1.0 3,106,085 + DvMF_2462 0.64 -0.0 3,106,093 - DvMF_2462 0.65 -0.8 3,106,311 + +1.3 3,106,336 + -0.1
Or see this region's nucleotide sequence