Experiment: MoYLS4 with Gentamicin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1489 and DvMF_1490 are separated by 578 nucleotides DvMF_1490 and DvMF_1491 are separated by 4 nucleotides DvMF_1491 and DvMF_1492 are separated by 325 nucleotides
DvMF_1489: DvMF_1489 - Lysine exporter protein (LYSE/YGGA) (RefSeq), at 1,826,834 to 1,827,526
_1489
DvMF_1490: DvMF_1490 - Nitrite reductase (cytochrome; ammonia-forming) (RefSeq), at 1,828,105 to 1,829,694
_1490
DvMF_1491: DvMF_1491 - cytochrome c nitrite reductase small subunit (RefSeq), at 1,829,699 to 1,830,178
_1491
DvMF_1492: DvMF_1492 - multi-sensor hybrid histidine kinase (RefSeq), at 1,830,504 to 1,833,593
_1492
Position (kb)
1828
1829
1830 Strain fitness (log2 ratio)
-1
0
1 at 1827.313 kb on - strand, within DvMF_1489 at 1827.417 kb on + strand, within DvMF_1489 at 1827.758 kb on + strand at 1827.771 kb on + strand at 1827.857 kb on - strand at 1828.221 kb on - strand at 1828.573 kb on - strand, within DvMF_1490 at 1828.708 kb on + strand, within DvMF_1490 at 1828.820 kb on + strand, within DvMF_1490 at 1828.820 kb on + strand, within DvMF_1490 at 1828.861 kb on - strand, within DvMF_1490 at 1828.885 kb on - strand, within DvMF_1490 at 1828.919 kb on + strand, within DvMF_1490 at 1828.927 kb on - strand, within DvMF_1490 at 1829.044 kb on - strand, within DvMF_1490 at 1829.044 kb on - strand, within DvMF_1490 at 1829.088 kb on + strand, within DvMF_1490 at 1829.309 kb on + strand, within DvMF_1490 at 1829.643 kb on - strand at 1829.886 kb on + strand, within DvMF_1491 at 1829.896 kb on + strand, within DvMF_1491 at 1829.921 kb on - strand, within DvMF_1491 at 1830.007 kb on - strand, within DvMF_1491 at 1830.490 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.03 mg/ml remove 1,827,313 - DvMF_1489 0.69 -0.3 1,827,417 + DvMF_1489 0.84 -0.4 1,827,758 + -0.3 1,827,771 + -0.7 1,827,857 - -0.8 1,828,221 - -0.6 1,828,573 - DvMF_1490 0.29 -0.3 1,828,708 + DvMF_1490 0.38 -0.4 1,828,820 + DvMF_1490 0.45 -0.3 1,828,820 + DvMF_1490 0.45 -0.8 1,828,861 - DvMF_1490 0.48 -0.8 1,828,885 - DvMF_1490 0.49 -0.5 1,828,919 + DvMF_1490 0.51 -0.5 1,828,927 - DvMF_1490 0.52 -0.3 1,829,044 - DvMF_1490 0.59 -0.5 1,829,044 - DvMF_1490 0.59 -0.8 1,829,088 + DvMF_1490 0.62 -0.6 1,829,309 + DvMF_1490 0.76 -0.3 1,829,643 - -0.6 1,829,886 + DvMF_1491 0.39 -0.4 1,829,896 + DvMF_1491 0.41 -0.7 1,829,921 - DvMF_1491 0.46 -0.9 1,830,007 - DvMF_1491 0.64 -0.8 1,830,490 + -1.1
Or see this region's nucleotide sequence