Experiment: MoYLS4 with Gentamicin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0625 and DvMF_0626 are separated by 77 nucleotides DvMF_0626 and DvMF_0627 are separated by 464 nucleotides DvMF_0627 and DvMF_0628 are separated by 598 nucleotides
DvMF_0625: DvMF_0625 - protein of unknown function DUF1641 (RefSeq), at 747,311 to 748,012
_0625
DvMF_0626: DvMF_0626 - response regulator receiver protein (RefSeq), at 748,090 to 748,476
_0626
DvMF_0627: DvMF_0627 - Sel1 domain protein repeat-containing protein (RefSeq), at 748,941 to 749,627
_0627
DvMF_0628: DvMF_0628 - protein of unknown function DUF214 (RefSeq), at 750,226 to 751,401
_0628
Position (kb) 748
749
750 Strain fitness (log2 ratio) -1
0
1 at 748.190 kb on + strand, within DvMF_0626 at 748.302 kb on + strand, within DvMF_0626 at 748.333 kb on - strand, within DvMF_0626 at 748.333 kb on - strand, within DvMF_0626 at 748.333 kb on - strand, within DvMF_0626 at 748.372 kb on - strand, within DvMF_0626 at 748.470 kb on + strand at 748.523 kb on + strand at 748.602 kb on + strand at 748.602 kb on + strand at 748.610 kb on - strand at 748.754 kb on + strand at 749.167 kb on + strand, within DvMF_0627 at 749.173 kb on + strand, within DvMF_0627 at 749.258 kb on + strand, within DvMF_0627 at 749.353 kb on - strand, within DvMF_0627 at 749.442 kb on + strand, within DvMF_0627 at 749.684 kb on - strand at 749.844 kb on + strand at 750.356 kb on - strand, within DvMF_0628 at 750.435 kb on - strand, within DvMF_0628 at 750.617 kb on + strand, within DvMF_0628
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.03 mg/ml remove 748,190 + DvMF_0626 0.26 -0.4 748,302 + DvMF_0626 0.55 -0.3 748,333 - DvMF_0626 0.63 -0.3 748,333 - DvMF_0626 0.63 -1.5 748,333 - DvMF_0626 0.63 -0.4 748,372 - DvMF_0626 0.73 +0.1 748,470 + -0.5 748,523 + -0.2 748,602 + -0.2 748,602 + -0.6 748,610 - -0.9 748,754 + +0.2 749,167 + DvMF_0627 0.33 -1.0 749,173 + DvMF_0627 0.34 -1.2 749,258 + DvMF_0627 0.46 -1.1 749,353 - DvMF_0627 0.60 -0.1 749,442 + DvMF_0627 0.73 -0.5 749,684 - -0.1 749,844 + -0.6 750,356 - DvMF_0628 0.11 -0.1 750,435 - DvMF_0628 0.18 +0.0 750,617 + DvMF_0628 0.33 -0.2
Or see this region's nucleotide sequence