Experiment: MoYLS4 with Gentamicin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysS and DvMF_0483 are separated by 8 nucleotides DvMF_0483 and DvMF_0484 are separated by 115 nucleotides DvMF_0484 and DvMF_0485 are separated by 100 nucleotides
DvMF_0482: cysS - cysteinyl-tRNA synthetase (RefSeq), at 565,960 to 567,417
cysS
DvMF_0483: DvMF_0483 - peptidase M48 Ste24p (RefSeq), at 567,426 to 568,898
_0483
DvMF_0484: DvMF_0484 - Appr-1-p processing domain protein (RefSeq), at 569,014 to 569,622
_0484
DvMF_0485: DvMF_0485 - ATP-dependent protease peptidase subunit (RefSeq), at 569,723 to 570,280
_0485
Position (kb) 567
568
569 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 567.476 kb on - strand at 567.519 kb on - strand at 567.547 kb on - strand at 567.770 kb on + strand, within DvMF_0483 at 567.923 kb on + strand, within DvMF_0483 at 567.999 kb on + strand, within DvMF_0483 at 568.162 kb on - strand, within DvMF_0483 at 568.207 kb on - strand, within DvMF_0483 at 568.225 kb on - strand, within DvMF_0483 at 568.522 kb on - strand, within DvMF_0483 at 568.697 kb on - strand, within DvMF_0483 at 569.117 kb on - strand, within DvMF_0484 at 569.266 kb on + strand, within DvMF_0484 at 569.360 kb on + strand, within DvMF_0484 at 569.463 kb on + strand, within DvMF_0484 at 569.471 kb on - strand, within DvMF_0484 at 569.471 kb on - strand, within DvMF_0484 at 569.585 kb on - strand at 569.665 kb on + strand at 569.679 kb on - strand at 569.799 kb on + strand, within DvMF_0485
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.03 mg/ml remove 567,476 - -0.5 567,519 - -2.2 567,547 - -1.7 567,770 + DvMF_0483 0.23 -2.0 567,923 + DvMF_0483 0.34 -1.6 567,999 + DvMF_0483 0.39 -1.5 568,162 - DvMF_0483 0.50 -1.9 568,207 - DvMF_0483 0.53 -1.3 568,225 - DvMF_0483 0.54 -1.8 568,522 - DvMF_0483 0.74 -1.1 568,697 - DvMF_0483 0.86 -3.2 569,117 - DvMF_0484 0.17 -0.0 569,266 + DvMF_0484 0.41 -0.4 569,360 + DvMF_0484 0.57 -0.9 569,463 + DvMF_0484 0.74 +0.1 569,471 - DvMF_0484 0.75 +0.1 569,471 - DvMF_0484 0.75 -1.2 569,585 - -0.1 569,665 + -0.3 569,679 - -0.8 569,799 + DvMF_0485 0.14 -0.6
Or see this region's nucleotide sequence