Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispD and ispF are separated by 51 nucleotides ispF and Shewana3_1120 are separated by 29 nucleotides Shewana3_1120 and surE overlap by 4 nucleotides surE and Shewana3_1122 overlap by 4 nucleotides
Shewana3_1118: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (RefSeq), at 1,317,457 to 1,318,206
ispD
Shewana3_1119: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (RefSeq), at 1,318,258 to 1,318,737
ispF
Shewana3_1120: Shewana3_1120 - tRNA pseudouridine synthase D, TruD (RefSeq), at 1,318,767 to 1,319,894
_1120
Shewana3_1121: surE - stationary phase survival protein SurE (RefSeq), at 1,319,891 to 1,320,640
surE
Shewana3_1122: Shewana3_1122 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,320,637 to 1,321,272
_1122
Position (kb)
1318
1319
1320 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1318.727 kb on + strand at 1318.941 kb on - strand, within Shewana3_1120 at 1319.026 kb on - strand, within Shewana3_1120 at 1319.066 kb on + strand, within Shewana3_1120 at 1319.074 kb on - strand, within Shewana3_1120 at 1319.146 kb on + strand, within Shewana3_1120 at 1319.262 kb on + strand, within Shewana3_1120 at 1319.331 kb on + strand, within Shewana3_1120 at 1319.333 kb on + strand, within Shewana3_1120 at 1319.350 kb on + strand, within Shewana3_1120 at 1319.584 kb on + strand, within Shewana3_1120 at 1319.876 kb on + strand at 1319.886 kb on + strand at 1319.978 kb on + strand, within surE at 1320.132 kb on - strand, within surE at 1320.232 kb on + strand, within surE at 1320.397 kb on + strand, within surE at 1320.433 kb on + strand, within surE at 1320.441 kb on - strand, within surE at 1320.578 kb on + strand at 1320.728 kb on + strand, within Shewana3_1122
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 1,318,727 + -1.0 1,318,941 - Shewana3_1120 0.15 -0.3 1,319,026 - Shewana3_1120 0.23 -3.4 1,319,066 + Shewana3_1120 0.27 -0.2 1,319,074 - Shewana3_1120 0.27 -1.9 1,319,146 + Shewana3_1120 0.34 -2.4 1,319,262 + Shewana3_1120 0.44 +0.2 1,319,331 + Shewana3_1120 0.50 +1.3 1,319,333 + Shewana3_1120 0.50 -0.0 1,319,350 + Shewana3_1120 0.52 -1.1 1,319,584 + Shewana3_1120 0.72 -1.3 1,319,876 + -1.2 1,319,886 + -2.9 1,319,978 + surE Shewana3_1121 0.12 -0.6 1,320,132 - surE Shewana3_1121 0.32 -2.3 1,320,232 + surE Shewana3_1121 0.45 -3.7 1,320,397 + surE Shewana3_1121 0.67 -3.3 1,320,433 + surE Shewana3_1121 0.72 -3.6 1,320,441 - surE Shewana3_1121 0.73 -5.5 1,320,578 + -3.6 1,320,728 + Shewana3_1122 0.14 -1.3
Or see this region's nucleotide sequence