Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1117 and ispD are separated by 10 nucleotides ispD and ispF are separated by 51 nucleotides ispF and Shewana3_1120 are separated by 29 nucleotides
Shewana3_1117: Shewana3_1117 - cell division protein FtsB (RefSeq), at 1,317,147 to 1,317,446
_1117
Shewana3_1118: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (RefSeq), at 1,317,457 to 1,318,206
ispD
Shewana3_1119: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (RefSeq), at 1,318,258 to 1,318,737
ispF
Shewana3_1120: Shewana3_1120 - tRNA pseudouridine synthase D, TruD (RefSeq), at 1,318,767 to 1,319,894
_1120
Position (kb)
1318
1319 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1318.727 kb on + strand at 1318.941 kb on - strand, within Shewana3_1120 at 1319.026 kb on - strand, within Shewana3_1120 at 1319.066 kb on + strand, within Shewana3_1120 at 1319.074 kb on - strand, within Shewana3_1120 at 1319.146 kb on + strand, within Shewana3_1120 at 1319.262 kb on + strand, within Shewana3_1120 at 1319.331 kb on + strand, within Shewana3_1120 at 1319.333 kb on + strand, within Shewana3_1120 at 1319.350 kb on + strand, within Shewana3_1120 at 1319.584 kb on + strand, within Shewana3_1120
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 1,318,727 + -1.0 1,318,941 - Shewana3_1120 0.15 -0.3 1,319,026 - Shewana3_1120 0.23 -3.4 1,319,066 + Shewana3_1120 0.27 -0.2 1,319,074 - Shewana3_1120 0.27 -1.9 1,319,146 + Shewana3_1120 0.34 -2.4 1,319,262 + Shewana3_1120 0.44 +0.2 1,319,331 + Shewana3_1120 0.50 +1.3 1,319,333 + Shewana3_1120 0.50 -0.0 1,319,350 + Shewana3_1120 0.52 -1.1 1,319,584 + Shewana3_1120 0.72 -1.3
Or see this region's nucleotide sequence