Experiment: succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1442 and Shew_1443 are separated by 2 nucleotides Shew_1443 and Shew_1444 are separated by 83 nucleotides
Shew_1442: Shew_1442 - two component, sigma54 specific, Fis family transcriptional regulator (RefSeq), at 1,657,319 to 1,658,686
_1442
Shew_1443: Shew_1443 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 1,658,689 to 1,660,512
_1443
Shew_1444: Shew_1444 - dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), large permease component (from data) , at 1,660,596 to 1,661,993
_1444
Position (kb)
1658
1659
1660
1661 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1657.888 kb on + strand, within Shew_1442 at 1658.082 kb on + strand, within Shew_1442 at 1658.245 kb on + strand, within Shew_1442 at 1658.359 kb on - strand, within Shew_1442 at 1658.545 kb on + strand, within Shew_1442 at 1658.545 kb on + strand, within Shew_1442 at 1659.028 kb on - strand, within Shew_1443 at 1659.748 kb on + strand, within Shew_1443 at 1659.929 kb on + strand, within Shew_1443 at 1659.969 kb on + strand, within Shew_1443 at 1660.215 kb on + strand, within Shew_1443 at 1660.243 kb on + strand, within Shew_1443 at 1660.307 kb on - strand, within Shew_1443 at 1660.439 kb on - strand at 1660.834 kb on + strand, within Shew_1444
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C) remove 1,657,888 + Shew_1442 0.42 -4.7 1,658,082 + Shew_1442 0.56 -4.1 1,658,245 + Shew_1442 0.68 -6.5 1,658,359 - Shew_1442 0.76 -2.0 1,658,545 + Shew_1442 0.90 -5.6 1,658,545 + Shew_1442 0.90 -2.3 1,659,028 - Shew_1443 0.19 -4.4 1,659,748 + Shew_1443 0.58 -7.1 1,659,929 + Shew_1443 0.68 -3.8 1,659,969 + Shew_1443 0.70 -6.0 1,660,215 + Shew_1443 0.84 -5.2 1,660,243 + Shew_1443 0.85 -4.7 1,660,307 - Shew_1443 0.89 -1.5 1,660,439 - -3.8 1,660,834 + Shew_1444 0.17 -4.6
Or see this region's nucleotide sequence