Experiment: succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1120 and Shew_1121 are separated by 53 nucleotides Shew_1121 and Shew_1122 are separated by 76 nucleotides Shew_1122 and Shew_1123 are separated by 2 nucleotides Shew_1123 and trmB are separated by 235 nucleotides trmB and Shew_1125 are separated by 30 nucleotides
Shew_1120: Shew_1120 - cytochrome B561 (RefSeq), at 1,296,554 to 1,297,108
_1120
Shew_1121: Shew_1121 - hypothetical protein (RefSeq), at 1,297,162 to 1,297,695
_1121
Shew_1122: Shew_1122 - hypothetical protein (RefSeq), at 1,297,772 to 1,298,050
_1122
Shew_1123: Shew_1123 - A/G-specific adenine glycosylase (RefSeq), at 1,298,053 to 1,299,159
_1123
Shew_1124: trmB - tRNA (guanine-N(7))-methyltransferase (RefSeq), at 1,299,395 to 1,300,111
trmB
Shew_1125: Shew_1125 - hypothetical protein (RefSeq), at 1,300,142 to 1,300,465
_1125
Position (kb)
1298
1299
1300 Strain fitness (log2 ratio)
-2
-1
0
1 at 1297.170 kb on + strand at 1297.249 kb on + strand, within Shew_1121 at 1297.406 kb on + strand, within Shew_1121 at 1297.436 kb on + strand, within Shew_1121 at 1297.814 kb on - strand, within Shew_1122 at 1297.983 kb on + strand, within Shew_1122 at 1298.279 kb on - strand, within Shew_1123 at 1298.533 kb on + strand, within Shew_1123 at 1298.781 kb on - strand, within Shew_1123 at 1298.920 kb on + strand, within Shew_1123 at 1298.943 kb on + strand, within Shew_1123 at 1298.944 kb on + strand, within Shew_1123 at 1299.009 kb on + strand, within Shew_1123 at 1299.419 kb on - strand at 1299.464 kb on - strand at 1299.740 kb on - strand, within trmB at 1299.801 kb on + strand, within trmB
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C) remove 1,297,170 + +1.1 1,297,249 + Shew_1121 0.16 +0.0 1,297,406 + Shew_1121 0.46 +0.2 1,297,436 + Shew_1121 0.51 +0.4 1,297,814 - Shew_1122 0.15 -1.2 1,297,983 + Shew_1122 0.76 -2.3 1,298,279 - Shew_1123 0.20 +1.1 1,298,533 + Shew_1123 0.43 +0.3 1,298,781 - Shew_1123 0.66 +1.0 1,298,920 + Shew_1123 0.78 -0.0 1,298,943 + Shew_1123 0.80 +0.2 1,298,944 + Shew_1123 0.80 +1.3 1,299,009 + Shew_1123 0.86 +0.9 1,299,419 - -0.5 1,299,464 - -0.6 1,299,740 - trmB Shew_1124 0.48 -0.7 1,299,801 + trmB Shew_1124 0.57 -1.0
Or see this region's nucleotide sequence