Experiment: D-Xylose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1451 and Pf6N2E2_1452 are separated by 71 nucleotides Pf6N2E2_1452 and Pf6N2E2_1453 are separated by 20 nucleotides Pf6N2E2_1453 and Pf6N2E2_1454 are separated by 155 nucleotides
Pf6N2E2_1451 - hypothetical protein, at 1,698,843 to 1,699,817
_1451
Pf6N2E2_1452 - hypothetical protein, at 1,699,889 to 1,700,431
_1452
Pf6N2E2_1453 - Xylose activator XylR (AraC family), at 1,700,452 to 1,701,639
_1453
Pf6N2E2_1454 - Xylose isomerase (EC 5.3.1.5), at 1,701,795 to 1,703,111
_1454
Position (kb)
1700
1701
1702 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1699.453 kb on + strand, within Pf6N2E2_1451 at 1699.453 kb on + strand, within Pf6N2E2_1451 at 1699.454 kb on - strand, within Pf6N2E2_1451 at 1699.457 kb on + strand, within Pf6N2E2_1451 at 1699.750 kb on - strand at 1699.750 kb on - strand at 1699.750 kb on - strand at 1699.750 kb on - strand at 1699.751 kb on + strand at 1699.752 kb on - strand at 1699.752 kb on - strand at 1699.752 kb on - strand at 1699.752 kb on - strand at 1699.753 kb on + strand at 1699.754 kb on - strand at 1699.754 kb on - strand at 1699.754 kb on - strand at 1699.754 kb on - strand at 1699.754 kb on - strand at 1699.763 kb on - strand at 1699.763 kb on - strand at 1699.852 kb on + strand at 1699.852 kb on + strand at 1699.853 kb on - strand at 1699.878 kb on + strand at 1699.878 kb on + strand at 1699.879 kb on - strand at 1699.879 kb on - strand at 1699.879 kb on - strand at 1699.879 kb on - strand at 1700.043 kb on - strand, within Pf6N2E2_1452 at 1700.132 kb on - strand, within Pf6N2E2_1452 at 1700.210 kb on - strand, within Pf6N2E2_1452 at 1700.239 kb on + strand, within Pf6N2E2_1452 at 1700.282 kb on - strand, within Pf6N2E2_1452 at 1700.351 kb on - strand, within Pf6N2E2_1452 at 1700.370 kb on + strand, within Pf6N2E2_1452 at 1700.386 kb on - strand at 1700.531 kb on + strand at 1700.531 kb on + strand at 1700.532 kb on - strand at 1700.532 kb on - strand at 1700.702 kb on + strand, within Pf6N2E2_1453 at 1700.702 kb on + strand, within Pf6N2E2_1453 at 1700.702 kb on + strand, within Pf6N2E2_1453 at 1700.703 kb on - strand, within Pf6N2E2_1453 at 1700.703 kb on - strand, within Pf6N2E2_1453 at 1700.741 kb on + strand, within Pf6N2E2_1453 at 1700.741 kb on + strand, within Pf6N2E2_1453 at 1700.774 kb on + strand, within Pf6N2E2_1453 at 1700.774 kb on + strand, within Pf6N2E2_1453 at 1700.774 kb on + strand, within Pf6N2E2_1453 at 1700.775 kb on - strand, within Pf6N2E2_1453 at 1700.775 kb on - strand, within Pf6N2E2_1453 at 1701.299 kb on + strand, within Pf6N2E2_1453 at 1701.299 kb on + strand, within Pf6N2E2_1453 at 1701.549 kb on - strand at 1701.645 kb on + strand at 1701.671 kb on - strand at 1701.695 kb on + strand at 1701.755 kb on - strand at 1701.777 kb on + strand at 1701.777 kb on + strand at 1701.778 kb on - strand at 1701.801 kb on + strand at 1701.802 kb on - strand at 1701.802 kb on - strand at 1701.802 kb on - strand at 1701.870 kb on + strand at 1701.870 kb on + strand at 1701.934 kb on - strand, within Pf6N2E2_1454 at 1701.941 kb on + strand, within Pf6N2E2_1454 at 1701.942 kb on - strand, within Pf6N2E2_1454 at 1701.942 kb on - strand, within Pf6N2E2_1454 at 1702.083 kb on + strand, within Pf6N2E2_1454 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.524 kb on + strand, within Pf6N2E2_1454 at 1702.525 kb on - strand, within Pf6N2E2_1454 at 1702.622 kb on + strand, within Pf6N2E2_1454 at 1702.623 kb on - strand, within Pf6N2E2_1454
Per-strain Table
Position Strand Gene LocusTag Fraction D-Xylose (C) remove 1,699,453 + Pf6N2E2_1451 0.63 +0.1 1,699,453 + Pf6N2E2_1451 0.63 -0.9 1,699,454 - Pf6N2E2_1451 0.63 +1.2 1,699,457 + Pf6N2E2_1451 0.63 +1.9 1,699,750 - +0.0 1,699,750 - +0.5 1,699,750 - +0.0 1,699,750 - -0.7 1,699,751 + +0.3 1,699,752 - -0.1 1,699,752 - -0.1 1,699,752 - +0.5 1,699,752 - -0.2 1,699,753 + -0.6 1,699,754 - +0.1 1,699,754 - +0.6 1,699,754 - +0.2 1,699,754 - -0.8 1,699,754 - -0.7 1,699,763 - -0.1 1,699,763 - -0.4 1,699,852 + -0.0 1,699,852 + +0.1 1,699,853 - +1.6 1,699,878 + +0.2 1,699,878 + -1.4 1,699,879 - +0.1 1,699,879 - +0.6 1,699,879 - +1.2 1,699,879 - -1.5 1,700,043 - Pf6N2E2_1452 0.28 +0.7 1,700,132 - Pf6N2E2_1452 0.45 +0.9 1,700,210 - Pf6N2E2_1452 0.59 +0.9 1,700,239 + Pf6N2E2_1452 0.64 +0.9 1,700,282 - Pf6N2E2_1452 0.72 -0.1 1,700,351 - Pf6N2E2_1452 0.85 +0.9 1,700,370 + Pf6N2E2_1452 0.89 +0.1 1,700,386 - +0.8 1,700,531 + -1.4 1,700,531 + -1.2 1,700,532 - -0.4 1,700,532 - -0.6 1,700,702 + Pf6N2E2_1453 0.21 +0.1 1,700,702 + Pf6N2E2_1453 0.21 -4.5 1,700,702 + Pf6N2E2_1453 0.21 -0.8 1,700,703 - Pf6N2E2_1453 0.21 -0.4 1,700,703 - Pf6N2E2_1453 0.21 -2.2 1,700,741 + Pf6N2E2_1453 0.24 -2.6 1,700,741 + Pf6N2E2_1453 0.24 -1.0 1,700,774 + Pf6N2E2_1453 0.27 -0.8 1,700,774 + Pf6N2E2_1453 0.27 -2.1 1,700,774 + Pf6N2E2_1453 0.27 +1.1 1,700,775 - Pf6N2E2_1453 0.27 -1.2 1,700,775 - Pf6N2E2_1453 0.27 -1.2 1,701,299 + Pf6N2E2_1453 0.71 -0.5 1,701,299 + Pf6N2E2_1453 0.71 -0.7 1,701,549 - +1.1 1,701,645 + -1.0 1,701,671 - -0.8 1,701,695 + -1.2 1,701,755 - +0.1 1,701,777 + +1.2 1,701,777 + +0.6 1,701,778 - -0.5 1,701,801 + -0.6 1,701,802 - -1.2 1,701,802 - -2.5 1,701,802 - +2.2 1,701,870 + -0.9 1,701,870 + +0.0 1,701,934 - Pf6N2E2_1454 0.11 -0.7 1,701,941 + Pf6N2E2_1454 0.11 -0.4 1,701,942 - Pf6N2E2_1454 0.11 -0.1 1,701,942 - Pf6N2E2_1454 0.11 -1.1 1,702,083 + Pf6N2E2_1454 0.22 -1.4 1,702,344 + Pf6N2E2_1454 0.42 +0.3 1,702,344 + Pf6N2E2_1454 0.42 -1.8 1,702,344 + Pf6N2E2_1454 0.42 -0.8 1,702,524 + Pf6N2E2_1454 0.55 -0.4 1,702,525 - Pf6N2E2_1454 0.55 +0.8 1,702,622 + Pf6N2E2_1454 0.63 -1.1 1,702,623 - Pf6N2E2_1454 0.63 -0.2
Or see this region's nucleotide sequence