Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25165 and CA265_RS25170 are separated by 83 nucleotides CA265_RS25170 and CA265_RS25175 are separated by 125 nucleotides
CA265_RS25165: CA265_RS25165 - glycosyl transferase, at 6,126,323 to 6,127,957
_RS25165
CA265_RS25170: CA265_RS25170 - pyridoxine 5'-phosphate synthase, at 6,128,041 to 6,128,757
_RS25170
CA265_RS25175: CA265_RS25175 - EamA family transporter, at 6,128,883 to 6,129,788
_RS25175
Position (kb)
6128
6129 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6127.138 kb on + strand, within CA265_RS25165 at 6127.139 kb on - strand, within CA265_RS25165 at 6127.139 kb on - strand, within CA265_RS25165 at 6127.139 kb on - strand, within CA265_RS25165 at 6127.141 kb on + strand, within CA265_RS25165 at 6127.168 kb on + strand, within CA265_RS25165 at 6127.169 kb on - strand, within CA265_RS25165 at 6127.176 kb on - strand, within CA265_RS25165 at 6127.257 kb on - strand, within CA265_RS25165 at 6127.257 kb on - strand, within CA265_RS25165 at 6127.286 kb on + strand, within CA265_RS25165 at 6127.287 kb on - strand, within CA265_RS25165 at 6127.287 kb on - strand, within CA265_RS25165 at 6127.292 kb on + strand, within CA265_RS25165 at 6127.292 kb on + strand, within CA265_RS25165 at 6127.293 kb on - strand, within CA265_RS25165 at 6127.331 kb on + strand, within CA265_RS25165 at 6127.334 kb on - strand, within CA265_RS25165 at 6127.385 kb on + strand, within CA265_RS25165 at 6127.399 kb on + strand, within CA265_RS25165 at 6127.400 kb on - strand, within CA265_RS25165 at 6127.400 kb on - strand, within CA265_RS25165 at 6127.430 kb on + strand, within CA265_RS25165 at 6127.430 kb on + strand, within CA265_RS25165 at 6127.430 kb on + strand, within CA265_RS25165 at 6127.431 kb on - strand, within CA265_RS25165 at 6127.444 kb on + strand, within CA265_RS25165 at 6127.510 kb on - strand, within CA265_RS25165 at 6127.518 kb on + strand, within CA265_RS25165 at 6127.519 kb on - strand, within CA265_RS25165 at 6127.519 kb on - strand, within CA265_RS25165 at 6127.523 kb on - strand, within CA265_RS25165 at 6127.548 kb on + strand, within CA265_RS25165 at 6127.549 kb on - strand, within CA265_RS25165 at 6127.549 kb on - strand, within CA265_RS25165 at 6127.636 kb on + strand, within CA265_RS25165 at 6127.637 kb on - strand, within CA265_RS25165 at 6127.638 kb on + strand, within CA265_RS25165 at 6127.638 kb on + strand, within CA265_RS25165 at 6127.639 kb on - strand, within CA265_RS25165 at 6127.639 kb on - strand, within CA265_RS25165 at 6127.639 kb on - strand, within CA265_RS25165 at 6127.659 kb on + strand, within CA265_RS25165 at 6127.659 kb on + strand, within CA265_RS25165 at 6127.800 kb on + strand at 6127.801 kb on - strand at 6127.804 kb on + strand at 6127.804 kb on + strand at 6127.875 kb on - strand at 6127.934 kb on + strand at 6127.972 kb on + strand at 6127.972 kb on + strand at 6128.030 kb on - strand at 6128.755 kb on + strand at 6128.816 kb on - strand at 6128.821 kb on - strand at 6128.880 kb on + strand at 6128.881 kb on - strand at 6128.882 kb on + strand at 6128.887 kb on + strand at 6128.939 kb on + strand at 6128.949 kb on - strand at 6128.972 kb on + strand at 6128.973 kb on - strand at 6129.131 kb on - strand, within CA265_RS25175 at 6129.131 kb on - strand, within CA265_RS25175 at 6129.147 kb on + strand, within CA265_RS25175 at 6129.229 kb on - strand, within CA265_RS25175 at 6129.260 kb on - strand at 6129.364 kb on + strand, within CA265_RS25175 at 6129.423 kb on + strand, within CA265_RS25175 at 6129.423 kb on + strand, within CA265_RS25175 at 6129.429 kb on - strand, within CA265_RS25175 at 6129.517 kb on + strand, within CA265_RS25175 at 6129.599 kb on - strand, within CA265_RS25175 at 6129.631 kb on + strand, within CA265_RS25175 at 6129.632 kb on - strand, within CA265_RS25175 at 6129.677 kb on - strand, within CA265_RS25175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 6,127,138 + CA265_RS25165 0.50 +0.5 6,127,139 - CA265_RS25165 0.50 +0.5 6,127,139 - CA265_RS25165 0.50 +1.0 6,127,139 - CA265_RS25165 0.50 +0.7 6,127,141 + CA265_RS25165 0.50 +0.0 6,127,168 + CA265_RS25165 0.52 -0.8 6,127,169 - CA265_RS25165 0.52 -1.5 6,127,176 - CA265_RS25165 0.52 +1.0 6,127,257 - CA265_RS25165 0.57 -0.4 6,127,257 - CA265_RS25165 0.57 +0.6 6,127,286 + CA265_RS25165 0.59 +0.7 6,127,287 - CA265_RS25165 0.59 +1.4 6,127,287 - CA265_RS25165 0.59 -0.5 6,127,292 + CA265_RS25165 0.59 +0.1 6,127,292 + CA265_RS25165 0.59 -1.5 6,127,293 - CA265_RS25165 0.59 +0.4 6,127,331 + CA265_RS25165 0.62 +0.9 6,127,334 - CA265_RS25165 0.62 +0.4 6,127,385 + CA265_RS25165 0.65 +0.4 6,127,399 + CA265_RS25165 0.66 +0.3 6,127,400 - CA265_RS25165 0.66 +1.2 6,127,400 - CA265_RS25165 0.66 +0.7 6,127,430 + CA265_RS25165 0.68 +0.1 6,127,430 + CA265_RS25165 0.68 -0.0 6,127,430 + CA265_RS25165 0.68 +1.1 6,127,431 - CA265_RS25165 0.68 -0.2 6,127,444 + CA265_RS25165 0.69 +2.8 6,127,510 - CA265_RS25165 0.73 +0.7 6,127,518 + CA265_RS25165 0.73 +0.5 6,127,519 - CA265_RS25165 0.73 +0.2 6,127,519 - CA265_RS25165 0.73 -0.2 6,127,523 - CA265_RS25165 0.73 -0.1 6,127,548 + CA265_RS25165 0.75 +0.2 6,127,549 - CA265_RS25165 0.75 +0.8 6,127,549 - CA265_RS25165 0.75 +0.7 6,127,636 + CA265_RS25165 0.80 +0.1 6,127,637 - CA265_RS25165 0.80 +0.1 6,127,638 + CA265_RS25165 0.80 +0.2 6,127,638 + CA265_RS25165 0.80 +0.5 6,127,639 - CA265_RS25165 0.80 +0.6 6,127,639 - CA265_RS25165 0.80 +0.3 6,127,639 - CA265_RS25165 0.80 -0.6 6,127,659 + CA265_RS25165 0.82 -0.3 6,127,659 + CA265_RS25165 0.82 +0.9 6,127,800 + +0.4 6,127,801 - +0.4 6,127,804 + +0.7 6,127,804 + +0.3 6,127,875 - +0.2 6,127,934 + +1.0 6,127,972 + +1.5 6,127,972 + +1.1 6,128,030 - -1.9 6,128,755 + +0.1 6,128,816 - +0.4 6,128,821 - +0.9 6,128,880 + -0.3 6,128,881 - +0.3 6,128,882 + +0.9 6,128,887 + +0.0 6,128,939 + +0.4 6,128,949 - +0.7 6,128,972 + -1.0 6,128,973 - +0.1 6,129,131 - CA265_RS25175 0.27 -0.4 6,129,131 - CA265_RS25175 0.27 -0.2 6,129,147 + CA265_RS25175 0.29 +0.1 6,129,229 - CA265_RS25175 0.38 +0.8 6,129,260 - +1.0 6,129,364 + CA265_RS25175 0.53 -1.0 6,129,423 + CA265_RS25175 0.60 -0.8 6,129,423 + CA265_RS25175 0.60 +0.7 6,129,429 - CA265_RS25175 0.60 +0.0 6,129,517 + CA265_RS25175 0.70 +0.3 6,129,599 - CA265_RS25175 0.79 +0.5 6,129,631 + CA265_RS25175 0.83 +0.3 6,129,632 - CA265_RS25175 0.83 +0.0 6,129,677 - CA265_RS25175 0.88 -0.9
Or see this region's nucleotide sequence