Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18220 and CA265_RS18225 are separated by 159 nucleotides CA265_RS18225 and CA265_RS18230 are separated by 78 nucleotides
CA265_RS18220: CA265_RS18220 - metallophosphatase, at 4,543,002 to 4,544,258
_RS18220
CA265_RS18225: CA265_RS18225 - DNA-binding response regulator, at 4,544,418 to 4,545,107
_RS18225
CA265_RS18230: CA265_RS18230 - two-component sensor histidine kinase, at 4,545,186 to 4,546,859
_RS18230
Position (kb)
4544
4545
4546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4543.501 kb on + strand, within CA265_RS18220 at 4543.714 kb on + strand, within CA265_RS18220 at 4543.715 kb on - strand, within CA265_RS18220 at 4543.753 kb on - strand, within CA265_RS18220 at 4543.800 kb on - strand, within CA265_RS18220 at 4543.801 kb on - strand, within CA265_RS18220 at 4543.814 kb on + strand, within CA265_RS18220 at 4543.815 kb on - strand, within CA265_RS18220 at 4543.819 kb on + strand, within CA265_RS18220 at 4543.820 kb on - strand, within CA265_RS18220 at 4543.967 kb on - strand, within CA265_RS18220 at 4543.973 kb on + strand, within CA265_RS18220 at 4543.974 kb on - strand, within CA265_RS18220 at 4543.974 kb on - strand, within CA265_RS18220 at 4543.979 kb on - strand, within CA265_RS18220 at 4543.979 kb on - strand, within CA265_RS18220 at 4543.981 kb on + strand, within CA265_RS18220 at 4544.061 kb on - strand, within CA265_RS18220 at 4544.119 kb on - strand, within CA265_RS18220 at 4544.180 kb on - strand at 4544.195 kb on - strand at 4544.237 kb on - strand at 4544.257 kb on - strand at 4544.257 kb on - strand at 4544.257 kb on - strand at 4544.257 kb on - strand at 4544.257 kb on - strand at 4544.366 kb on + strand at 4544.371 kb on + strand at 4544.372 kb on - strand at 4544.379 kb on + strand at 4544.379 kb on + strand at 4544.380 kb on - strand at 4544.380 kb on - strand at 4544.411 kb on - strand at 4544.413 kb on + strand at 4544.413 kb on + strand at 4544.414 kb on - strand at 4544.415 kb on + strand at 4544.442 kb on + strand at 4544.443 kb on - strand at 4544.490 kb on + strand, within CA265_RS18225 at 4544.513 kb on + strand, within CA265_RS18225 at 4544.513 kb on + strand, within CA265_RS18225 at 4544.535 kb on - strand, within CA265_RS18225 at 4544.538 kb on + strand, within CA265_RS18225 at 4544.571 kb on + strand, within CA265_RS18225 at 4544.643 kb on + strand, within CA265_RS18225 at 4544.699 kb on + strand, within CA265_RS18225 at 4544.699 kb on + strand, within CA265_RS18225 at 4544.699 kb on + strand, within CA265_RS18225 at 4544.700 kb on - strand, within CA265_RS18225 at 4544.703 kb on - strand, within CA265_RS18225 at 4544.802 kb on - strand, within CA265_RS18225 at 4544.804 kb on - strand, within CA265_RS18225 at 4544.824 kb on + strand, within CA265_RS18225 at 4544.868 kb on - strand, within CA265_RS18225 at 4544.871 kb on - strand, within CA265_RS18225 at 4544.946 kb on + strand, within CA265_RS18225 at 4544.957 kb on + strand, within CA265_RS18225 at 4544.957 kb on + strand, within CA265_RS18225 at 4545.021 kb on - strand, within CA265_RS18225 at 4545.021 kb on - strand, within CA265_RS18225 at 4545.037 kb on - strand, within CA265_RS18225 at 4545.078 kb on + strand at 4545.112 kb on - strand at 4545.120 kb on + strand at 4545.173 kb on + strand at 4545.173 kb on + strand at 4545.194 kb on + strand at 4545.194 kb on + strand at 4545.196 kb on + strand at 4545.196 kb on + strand at 4545.196 kb on + strand at 4545.231 kb on + strand at 4545.323 kb on - strand at 4545.354 kb on + strand, within CA265_RS18230 at 4545.475 kb on + strand, within CA265_RS18230 at 4545.502 kb on - strand, within CA265_RS18230 at 4545.530 kb on + strand, within CA265_RS18230 at 4545.540 kb on - strand, within CA265_RS18230 at 4545.556 kb on + strand, within CA265_RS18230 at 4545.557 kb on - strand, within CA265_RS18230 at 4545.623 kb on + strand, within CA265_RS18230 at 4545.624 kb on - strand, within CA265_RS18230 at 4545.624 kb on - strand, within CA265_RS18230 at 4545.648 kb on - strand, within CA265_RS18230 at 4545.671 kb on + strand, within CA265_RS18230 at 4545.693 kb on + strand, within CA265_RS18230 at 4545.693 kb on + strand, within CA265_RS18230 at 4545.726 kb on + strand, within CA265_RS18230 at 4545.726 kb on + strand, within CA265_RS18230 at 4545.768 kb on - strand, within CA265_RS18230 at 4545.872 kb on + strand, within CA265_RS18230 at 4545.926 kb on - strand, within CA265_RS18230 at 4545.928 kb on - strand, within CA265_RS18230 at 4546.013 kb on + strand, within CA265_RS18230 at 4546.014 kb on - strand, within CA265_RS18230 at 4546.089 kb on + strand, within CA265_RS18230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 4,543,501 + CA265_RS18220 0.40 -0.8 4,543,714 + CA265_RS18220 0.57 -0.3 4,543,715 - CA265_RS18220 0.57 -1.0 4,543,753 - CA265_RS18220 0.60 -0.8 4,543,800 - CA265_RS18220 0.63 +0.8 4,543,801 - CA265_RS18220 0.64 +0.6 4,543,814 + CA265_RS18220 0.65 -1.0 4,543,815 - CA265_RS18220 0.65 -0.3 4,543,819 + CA265_RS18220 0.65 -1.2 4,543,820 - CA265_RS18220 0.65 +0.5 4,543,967 - CA265_RS18220 0.77 -0.9 4,543,973 + CA265_RS18220 0.77 +1.5 4,543,974 - CA265_RS18220 0.77 -0.6 4,543,974 - CA265_RS18220 0.77 -1.3 4,543,979 - CA265_RS18220 0.78 +0.3 4,543,979 - CA265_RS18220 0.78 +0.1 4,543,981 + CA265_RS18220 0.78 +0.2 4,544,061 - CA265_RS18220 0.84 +0.1 4,544,119 - CA265_RS18220 0.89 +0.1 4,544,180 - -0.5 4,544,195 - -0.4 4,544,237 - -0.4 4,544,257 - -0.5 4,544,257 - +1.1 4,544,257 - +0.4 4,544,257 - -0.0 4,544,257 - +0.8 4,544,366 + +0.8 4,544,371 + +0.8 4,544,372 - -0.5 4,544,379 + +0.2 4,544,379 + +0.0 4,544,380 - +1.0 4,544,380 - +0.0 4,544,411 - -1.3 4,544,413 + +1.0 4,544,413 + +1.1 4,544,414 - +0.7 4,544,415 + -0.8 4,544,442 + -2.4 4,544,443 - -2.3 4,544,490 + CA265_RS18225 0.10 -0.4 4,544,513 + CA265_RS18225 0.14 -1.1 4,544,513 + CA265_RS18225 0.14 -0.1 4,544,535 - CA265_RS18225 0.17 +1.1 4,544,538 + CA265_RS18225 0.17 -1.2 4,544,571 + CA265_RS18225 0.22 -1.8 4,544,643 + CA265_RS18225 0.33 -1.9 4,544,699 + CA265_RS18225 0.41 -2.5 4,544,699 + CA265_RS18225 0.41 -1.6 4,544,699 + CA265_RS18225 0.41 -2.9 4,544,700 - CA265_RS18225 0.41 +0.2 4,544,703 - CA265_RS18225 0.41 +0.0 4,544,802 - CA265_RS18225 0.56 +1.5 4,544,804 - CA265_RS18225 0.56 -0.3 4,544,824 + CA265_RS18225 0.59 -1.8 4,544,868 - CA265_RS18225 0.65 -2.0 4,544,871 - CA265_RS18225 0.66 -1.6 4,544,946 + CA265_RS18225 0.77 -0.3 4,544,957 + CA265_RS18225 0.78 -1.5 4,544,957 + CA265_RS18225 0.78 -1.9 4,545,021 - CA265_RS18225 0.87 -1.7 4,545,021 - CA265_RS18225 0.87 -0.5 4,545,037 - CA265_RS18225 0.90 -0.3 4,545,078 + -0.8 4,545,112 - +0.8 4,545,120 + -0.6 4,545,173 + -3.3 4,545,173 + -0.4 4,545,194 + -1.2 4,545,194 + -2.1 4,545,196 + -1.6 4,545,196 + -1.6 4,545,196 + -2.5 4,545,231 + -0.1 4,545,323 - +4.0 4,545,354 + CA265_RS18230 0.10 -1.5 4,545,475 + CA265_RS18230 0.17 -3.1 4,545,502 - CA265_RS18230 0.19 +4.9 4,545,530 + CA265_RS18230 0.21 -1.5 4,545,540 - CA265_RS18230 0.21 +4.7 4,545,556 + CA265_RS18230 0.22 -0.4 4,545,557 - CA265_RS18230 0.22 +4.3 4,545,623 + CA265_RS18230 0.26 -1.9 4,545,624 - CA265_RS18230 0.26 +4.6 4,545,624 - CA265_RS18230 0.26 +4.2 4,545,648 - CA265_RS18230 0.28 +4.0 4,545,671 + CA265_RS18230 0.29 -0.4 4,545,693 + CA265_RS18230 0.30 +2.8 4,545,693 + CA265_RS18230 0.30 -1.3 4,545,726 + CA265_RS18230 0.32 +0.4 4,545,726 + CA265_RS18230 0.32 -0.9 4,545,768 - CA265_RS18230 0.35 +2.9 4,545,872 + CA265_RS18230 0.41 -2.4 4,545,926 - CA265_RS18230 0.44 +4.7 4,545,928 - CA265_RS18230 0.44 +4.7 4,546,013 + CA265_RS18230 0.49 -0.2 4,546,014 - CA265_RS18230 0.49 +4.2 4,546,089 + CA265_RS18230 0.54 -0.2
Or see this region's nucleotide sequence