Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15780 and CA265_RS15785 are separated by 605 nucleotides CA265_RS15785 and CA265_RS15790 are separated by 100 nucleotides CA265_RS15790 and CA265_RS15795 are separated by 246 nucleotides
CA265_RS15780: CA265_RS15780 - hypothetical protein, at 3,972,590 to 3,973,540
_RS15780
CA265_RS15785: CA265_RS15785 - branched-chain-amino-acid transaminase, at 3,974,146 to 3,975,036
_RS15785
CA265_RS15790: CA265_RS15790 - SAM-dependent methyltransferase, at 3,975,137 to 3,975,715
_RS15790
CA265_RS15795: CA265_RS15795 - dihydroxy-acid dehydratase, at 3,975,962 to 3,977,644
_RS15795
Position (kb)
3974
3975
3976 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3973.175 kb on - strand, within CA265_RS15780 at 3973.377 kb on + strand, within CA265_RS15780 at 3973.382 kb on - strand, within CA265_RS15780 at 3973.382 kb on - strand, within CA265_RS15780 at 3973.416 kb on - strand, within CA265_RS15780 at 3973.416 kb on - strand, within CA265_RS15780 at 3973.547 kb on - strand at 3973.620 kb on + strand at 3973.621 kb on - strand at 3973.797 kb on - strand at 3973.962 kb on + strand at 3973.962 kb on + strand at 3973.964 kb on + strand at 3973.991 kb on - strand at 3974.038 kb on + strand at 3974.087 kb on - strand at 3974.089 kb on - strand at 3974.155 kb on + strand at 3974.157 kb on + strand at 3974.172 kb on + strand at 3974.248 kb on + strand, within CA265_RS15785 at 3974.249 kb on - strand, within CA265_RS15785 at 3974.330 kb on + strand, within CA265_RS15785 at 3974.374 kb on - strand, within CA265_RS15785 at 3974.400 kb on + strand, within CA265_RS15785 at 3974.401 kb on - strand, within CA265_RS15785 at 3974.434 kb on + strand, within CA265_RS15785 at 3974.435 kb on - strand, within CA265_RS15785 at 3974.435 kb on - strand, within CA265_RS15785 at 3974.437 kb on - strand, within CA265_RS15785 at 3974.665 kb on + strand, within CA265_RS15785 at 3974.689 kb on - strand, within CA265_RS15785 at 3974.725 kb on - strand, within CA265_RS15785 at 3974.801 kb on + strand, within CA265_RS15785 at 3974.828 kb on + strand, within CA265_RS15785 at 3974.828 kb on + strand, within CA265_RS15785 at 3974.850 kb on - strand, within CA265_RS15785 at 3974.856 kb on - strand, within CA265_RS15785 at 3974.856 kb on - strand, within CA265_RS15785 at 3974.856 kb on - strand, within CA265_RS15785 at 3974.865 kb on + strand, within CA265_RS15785 at 3974.866 kb on - strand, within CA265_RS15785 at 3974.910 kb on - strand, within CA265_RS15785 at 3975.020 kb on - strand at 3975.052 kb on + strand at 3975.053 kb on - strand at 3975.084 kb on + strand at 3975.085 kb on - strand at 3975.185 kb on - strand at 3975.278 kb on - strand, within CA265_RS15790 at 3975.347 kb on - strand, within CA265_RS15790 at 3975.566 kb on - strand, within CA265_RS15790 at 3975.577 kb on + strand, within CA265_RS15790 at 3975.749 kb on + strand at 3975.756 kb on + strand at 3975.786 kb on + strand at 3975.787 kb on - strand at 3975.986 kb on - strand at 3975.995 kb on - strand at 3975.995 kb on - strand at 3976.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 3,973,175 - CA265_RS15780 0.62 +1.1 3,973,377 + CA265_RS15780 0.83 -0.9 3,973,382 - CA265_RS15780 0.83 +0.5 3,973,382 - CA265_RS15780 0.83 +1.2 3,973,416 - CA265_RS15780 0.87 +1.7 3,973,416 - CA265_RS15780 0.87 +1.8 3,973,547 - +0.5 3,973,620 + -0.7 3,973,621 - +1.9 3,973,797 - +0.6 3,973,962 + -0.1 3,973,962 + +0.4 3,973,964 + -1.6 3,973,991 - +1.0 3,974,038 + -0.6 3,974,087 - +1.1 3,974,089 - +0.8 3,974,155 + -2.8 3,974,157 + -1.4 3,974,172 + -2.0 3,974,248 + CA265_RS15785 0.11 -2.1 3,974,249 - CA265_RS15785 0.12 +2.5 3,974,330 + CA265_RS15785 0.21 -2.0 3,974,374 - CA265_RS15785 0.26 +1.8 3,974,400 + CA265_RS15785 0.29 -1.5 3,974,401 - CA265_RS15785 0.29 +1.0 3,974,434 + CA265_RS15785 0.32 -1.4 3,974,435 - CA265_RS15785 0.32 -0.2 3,974,435 - CA265_RS15785 0.32 +1.6 3,974,437 - CA265_RS15785 0.33 +1.5 3,974,665 + CA265_RS15785 0.58 -1.5 3,974,689 - CA265_RS15785 0.61 +1.3 3,974,725 - CA265_RS15785 0.65 +1.7 3,974,801 + CA265_RS15785 0.74 -2.3 3,974,828 + CA265_RS15785 0.77 +1.5 3,974,828 + CA265_RS15785 0.77 -2.3 3,974,850 - CA265_RS15785 0.79 +0.5 3,974,856 - CA265_RS15785 0.80 +0.8 3,974,856 - CA265_RS15785 0.80 +0.7 3,974,856 - CA265_RS15785 0.80 +0.9 3,974,865 + CA265_RS15785 0.81 -2.0 3,974,866 - CA265_RS15785 0.81 +1.5 3,974,910 - CA265_RS15785 0.86 +0.3 3,975,020 - +1.4 3,975,052 + +0.5 3,975,053 - +0.6 3,975,084 + -2.3 3,975,085 - -0.6 3,975,185 - +0.5 3,975,278 - CA265_RS15790 0.24 +1.0 3,975,347 - CA265_RS15790 0.36 -0.1 3,975,566 - CA265_RS15790 0.74 +0.4 3,975,577 + CA265_RS15790 0.76 -0.3 3,975,749 + -2.8 3,975,756 + -0.8 3,975,786 + -1.9 3,975,787 - +2.0 3,975,986 - +1.7 3,975,995 - +3.0 3,975,995 - +5.5 3,976,028 - +1.4
Or see this region's nucleotide sequence