Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12780 and CA265_RS12785 are separated by 11 nucleotides CA265_RS12785 and CA265_RS12790 are separated by 282 nucleotides CA265_RS12790 and CA265_RS12795 are separated by 53 nucleotides
CA265_RS12780: CA265_RS12780 - hypothetical protein, at 3,185,897 to 3,186,577
_RS12780
CA265_RS12785: CA265_RS12785 - hypothetical protein, at 3,186,589 to 3,187,086
_RS12785
CA265_RS12790: CA265_RS12790 - hypothetical protein, at 3,187,369 to 3,188,004
_RS12790
CA265_RS12795: CA265_RS12795 - MFS transporter, at 3,188,058 to 3,189,260
_RS12795
Position (kb)
3187
3188
3189 Strain fitness (log2 ratio)
-1
0
1
2 at 3186.538 kb on + strand at 3186.629 kb on + strand at 3186.629 kb on + strand at 3186.647 kb on + strand, within CA265_RS12785 at 3186.692 kb on + strand, within CA265_RS12785 at 3186.700 kb on + strand, within CA265_RS12785 at 3186.700 kb on + strand, within CA265_RS12785 at 3186.701 kb on - strand, within CA265_RS12785 at 3186.701 kb on - strand, within CA265_RS12785 at 3186.701 kb on - strand, within CA265_RS12785 at 3186.702 kb on + strand, within CA265_RS12785 at 3186.710 kb on + strand, within CA265_RS12785 at 3186.711 kb on - strand, within CA265_RS12785 at 3186.741 kb on + strand, within CA265_RS12785 at 3186.764 kb on - strand, within CA265_RS12785 at 3186.767 kb on - strand, within CA265_RS12785 at 3186.830 kb on - strand, within CA265_RS12785 at 3186.833 kb on - strand, within CA265_RS12785 at 3186.833 kb on - strand, within CA265_RS12785 at 3186.833 kb on - strand, within CA265_RS12785 at 3186.842 kb on + strand, within CA265_RS12785 at 3186.964 kb on - strand, within CA265_RS12785 at 3186.966 kb on + strand, within CA265_RS12785 at 3186.966 kb on + strand, within CA265_RS12785 at 3186.994 kb on - strand, within CA265_RS12785 at 3186.994 kb on - strand, within CA265_RS12785 at 3187.057 kb on + strand at 3187.058 kb on - strand at 3187.097 kb on - strand at 3187.097 kb on - strand at 3187.118 kb on + strand at 3187.165 kb on - strand at 3187.169 kb on - strand at 3187.190 kb on - strand at 3187.248 kb on - strand at 3187.272 kb on - strand at 3187.345 kb on + strand at 3187.382 kb on + strand at 3187.385 kb on + strand at 3187.386 kb on - strand at 3187.467 kb on + strand, within CA265_RS12790 at 3187.471 kb on - strand, within CA265_RS12790 at 3187.522 kb on + strand, within CA265_RS12790 at 3187.618 kb on - strand, within CA265_RS12790 at 3187.618 kb on - strand, within CA265_RS12790 at 3187.657 kb on + strand, within CA265_RS12790 at 3187.667 kb on - strand, within CA265_RS12790 at 3187.667 kb on - strand, within CA265_RS12790 at 3187.679 kb on + strand, within CA265_RS12790 at 3187.719 kb on + strand, within CA265_RS12790 at 3187.720 kb on - strand, within CA265_RS12790 at 3187.724 kb on - strand, within CA265_RS12790 at 3187.725 kb on + strand, within CA265_RS12790 at 3187.743 kb on - strand, within CA265_RS12790 at 3187.752 kb on + strand, within CA265_RS12790 at 3187.753 kb on - strand, within CA265_RS12790 at 3187.757 kb on - strand, within CA265_RS12790 at 3187.758 kb on + strand, within CA265_RS12790 at 3187.795 kb on - strand, within CA265_RS12790 at 3187.872 kb on - strand, within CA265_RS12790 at 3187.902 kb on + strand, within CA265_RS12790 at 3187.916 kb on - strand, within CA265_RS12790 at 3187.934 kb on - strand, within CA265_RS12790 at 3187.966 kb on - strand at 3187.990 kb on + strand at 3187.990 kb on + strand at 3187.990 kb on + strand at 3187.991 kb on - strand at 3187.997 kb on + strand at 3188.019 kb on - strand at 3188.100 kb on + strand at 3188.137 kb on - strand at 3188.137 kb on - strand at 3188.140 kb on + strand at 3188.173 kb on - strand at 3188.337 kb on + strand, within CA265_RS12795 at 3188.525 kb on - strand, within CA265_RS12795 at 3188.557 kb on + strand, within CA265_RS12795 at 3188.558 kb on - strand, within CA265_RS12795 at 3188.564 kb on - strand, within CA265_RS12795 at 3188.586 kb on + strand, within CA265_RS12795 at 3188.612 kb on + strand, within CA265_RS12795 at 3188.612 kb on + strand, within CA265_RS12795 at 3188.612 kb on + strand at 3188.694 kb on + strand, within CA265_RS12795 at 3188.695 kb on - strand, within CA265_RS12795 at 3188.698 kb on + strand, within CA265_RS12795 at 3188.698 kb on + strand, within CA265_RS12795 at 3188.699 kb on - strand, within CA265_RS12795 at 3188.699 kb on - strand, within CA265_RS12795 at 3188.729 kb on + strand, within CA265_RS12795 at 3188.788 kb on - strand, within CA265_RS12795 at 3188.937 kb on + strand, within CA265_RS12795 at 3188.937 kb on + strand, within CA265_RS12795
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 3,186,538 + -0.7 3,186,629 + -0.1 3,186,629 + +1.3 3,186,647 + CA265_RS12785 0.12 +0.1 3,186,692 + CA265_RS12785 0.21 +0.3 3,186,700 + CA265_RS12785 0.22 -0.5 3,186,700 + CA265_RS12785 0.22 -0.2 3,186,701 - CA265_RS12785 0.22 -0.1 3,186,701 - CA265_RS12785 0.22 +1.0 3,186,701 - CA265_RS12785 0.22 +0.2 3,186,702 + CA265_RS12785 0.23 +0.4 3,186,710 + CA265_RS12785 0.24 +1.8 3,186,711 - CA265_RS12785 0.24 +0.6 3,186,741 + CA265_RS12785 0.31 +1.0 3,186,764 - CA265_RS12785 0.35 -0.7 3,186,767 - CA265_RS12785 0.36 +0.1 3,186,830 - CA265_RS12785 0.48 -0.6 3,186,833 - CA265_RS12785 0.49 +0.4 3,186,833 - CA265_RS12785 0.49 +0.2 3,186,833 - CA265_RS12785 0.49 -0.0 3,186,842 + CA265_RS12785 0.51 +0.4 3,186,964 - CA265_RS12785 0.75 -1.0 3,186,966 + CA265_RS12785 0.76 +0.0 3,186,966 + CA265_RS12785 0.76 -0.5 3,186,994 - CA265_RS12785 0.81 -0.2 3,186,994 - CA265_RS12785 0.81 +0.3 3,187,057 + +0.2 3,187,058 - -0.1 3,187,097 - +0.1 3,187,097 - -0.5 3,187,118 + +1.5 3,187,165 - +0.6 3,187,169 - -0.3 3,187,190 - -1.0 3,187,248 - +0.6 3,187,272 - +0.7 3,187,345 + -0.5 3,187,382 + +1.0 3,187,385 + -0.0 3,187,386 - +0.8 3,187,467 + CA265_RS12790 0.15 +0.4 3,187,471 - CA265_RS12790 0.16 +0.3 3,187,522 + CA265_RS12790 0.24 -0.2 3,187,618 - CA265_RS12790 0.39 +0.8 3,187,618 - CA265_RS12790 0.39 -0.1 3,187,657 + CA265_RS12790 0.45 -0.8 3,187,667 - CA265_RS12790 0.47 -0.1 3,187,667 - CA265_RS12790 0.47 +0.3 3,187,679 + CA265_RS12790 0.49 +1.9 3,187,719 + CA265_RS12790 0.55 -0.3 3,187,720 - CA265_RS12790 0.55 +1.1 3,187,724 - CA265_RS12790 0.56 +0.2 3,187,725 + CA265_RS12790 0.56 -0.3 3,187,743 - CA265_RS12790 0.59 +1.1 3,187,752 + CA265_RS12790 0.60 -0.3 3,187,753 - CA265_RS12790 0.60 +1.5 3,187,757 - CA265_RS12790 0.61 +1.0 3,187,758 + CA265_RS12790 0.61 +1.7 3,187,795 - CA265_RS12790 0.67 -0.0 3,187,872 - CA265_RS12790 0.79 +0.7 3,187,902 + CA265_RS12790 0.84 +0.8 3,187,916 - CA265_RS12790 0.86 -0.2 3,187,934 - CA265_RS12790 0.89 -0.1 3,187,966 - -0.7 3,187,990 + -0.8 3,187,990 + +0.2 3,187,990 + +0.2 3,187,991 - +1.1 3,187,997 + +0.5 3,188,019 - -0.7 3,188,100 + -0.7 3,188,137 - +0.7 3,188,137 - +0.5 3,188,140 + -0.9 3,188,173 - +0.2 3,188,337 + CA265_RS12795 0.23 -0.2 3,188,525 - CA265_RS12795 0.39 +1.0 3,188,557 + CA265_RS12795 0.41 -0.1 3,188,558 - CA265_RS12795 0.42 +0.7 3,188,564 - CA265_RS12795 0.42 -0.1 3,188,586 + CA265_RS12795 0.44 -1.3 3,188,612 + CA265_RS12795 0.46 +0.4 3,188,612 + CA265_RS12795 0.46 -0.2 3,188,612 + +0.5 3,188,694 + CA265_RS12795 0.53 -0.0 3,188,695 - CA265_RS12795 0.53 +0.1 3,188,698 + CA265_RS12795 0.53 +0.2 3,188,698 + CA265_RS12795 0.53 -0.8 3,188,699 - CA265_RS12795 0.53 +0.5 3,188,699 - CA265_RS12795 0.53 +2.1 3,188,729 + CA265_RS12795 0.56 +0.1 3,188,788 - CA265_RS12795 0.61 +0.6 3,188,937 + CA265_RS12795 0.73 -0.1 3,188,937 + CA265_RS12795 0.73 +0.3
Or see this region's nucleotide sequence