Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10655 and CA265_RS10660 overlap by 29 nucleotides CA265_RS10660 and CA265_RS10665 are separated by 73 nucleotides CA265_RS10665 and CA265_RS10670 are separated by 384 nucleotides
CA265_RS10655: CA265_RS10655 - hypothetical protein, at 2,633,692 to 2,634,321
_RS10655
CA265_RS10660: CA265_RS10660 - acyl dehydratase, at 2,634,293 to 2,634,754
_RS10660
CA265_RS10665: CA265_RS10665 - Rossman fold protein, TIGR00730 family, at 2,634,828 to 2,635,421
_RS10665
CA265_RS10670: CA265_RS10670 - TonB-dependent receptor, at 2,635,806 to 2,638,385
_RS10670
Position (kb)
2634
2635
2636 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2633.832 kb on + strand, within CA265_RS10655 at 2633.917 kb on - strand, within CA265_RS10655 at 2633.932 kb on - strand, within CA265_RS10655 at 2633.933 kb on + strand, within CA265_RS10655 at 2634.054 kb on + strand, within CA265_RS10655 at 2634.086 kb on + strand, within CA265_RS10655 at 2634.109 kb on + strand, within CA265_RS10655 at 2634.109 kb on + strand, within CA265_RS10655 at 2634.110 kb on - strand, within CA265_RS10655 at 2634.110 kb on - strand, within CA265_RS10655 at 2634.110 kb on - strand, within CA265_RS10655 at 2634.110 kb on - strand, within CA265_RS10655 at 2634.197 kb on - strand, within CA265_RS10655 at 2634.294 kb on + strand at 2634.295 kb on - strand at 2634.331 kb on + strand at 2634.386 kb on - strand, within CA265_RS10660 at 2634.389 kb on + strand, within CA265_RS10660 at 2634.624 kb on - strand, within CA265_RS10660 at 2634.638 kb on + strand, within CA265_RS10660 at 2634.682 kb on + strand, within CA265_RS10660 at 2634.717 kb on + strand at 2634.743 kb on - strand at 2634.764 kb on - strand at 2634.812 kb on + strand at 2634.812 kb on + strand at 2634.818 kb on + strand at 2634.819 kb on - strand at 2635.008 kb on + strand, within CA265_RS10665 at 2635.015 kb on - strand, within CA265_RS10665 at 2635.015 kb on - strand, within CA265_RS10665 at 2635.017 kb on + strand, within CA265_RS10665 at 2635.017 kb on + strand, within CA265_RS10665 at 2635.032 kb on - strand, within CA265_RS10665 at 2635.101 kb on + strand, within CA265_RS10665 at 2635.101 kb on + strand, within CA265_RS10665 at 2635.102 kb on - strand, within CA265_RS10665 at 2635.224 kb on + strand, within CA265_RS10665 at 2635.225 kb on - strand, within CA265_RS10665 at 2635.255 kb on - strand, within CA265_RS10665 at 2635.323 kb on + strand, within CA265_RS10665 at 2635.348 kb on - strand, within CA265_RS10665 at 2635.415 kb on + strand at 2635.487 kb on - strand at 2635.493 kb on - strand at 2635.537 kb on - strand at 2635.557 kb on - strand at 2635.563 kb on + strand at 2635.602 kb on + strand at 2635.682 kb on + strand at 2635.746 kb on + strand at 2635.777 kb on - strand at 2635.807 kb on + strand at 2635.808 kb on - strand at 2635.834 kb on + strand at 2635.864 kb on + strand at 2635.864 kb on + strand at 2635.935 kb on + strand at 2635.935 kb on + strand at 2635.983 kb on + strand at 2635.984 kb on - strand at 2636.005 kb on - strand at 2636.008 kb on - strand at 2636.022 kb on - strand at 2636.071 kb on + strand, within CA265_RS10670 at 2636.096 kb on + strand, within CA265_RS10670 at 2636.096 kb on + strand, within CA265_RS10670 at 2636.142 kb on + strand, within CA265_RS10670 at 2636.157 kb on - strand, within CA265_RS10670 at 2636.174 kb on + strand, within CA265_RS10670 at 2636.264 kb on + strand, within CA265_RS10670 at 2636.264 kb on + strand, within CA265_RS10670 at 2636.264 kb on + strand, within CA265_RS10670 at 2636.272 kb on - strand, within CA265_RS10670 at 2636.293 kb on - strand, within CA265_RS10670
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 2,633,832 + CA265_RS10655 0.22 -0.1 2,633,917 - CA265_RS10655 0.36 +0.5 2,633,932 - CA265_RS10655 0.38 +0.1 2,633,933 + CA265_RS10655 0.38 +0.2 2,634,054 + CA265_RS10655 0.57 +0.1 2,634,086 + CA265_RS10655 0.63 +1.0 2,634,109 + CA265_RS10655 0.66 +0.6 2,634,109 + CA265_RS10655 0.66 -0.4 2,634,110 - CA265_RS10655 0.66 -0.8 2,634,110 - CA265_RS10655 0.66 -0.3 2,634,110 - CA265_RS10655 0.66 +0.3 2,634,110 - CA265_RS10655 0.66 -0.6 2,634,197 - CA265_RS10655 0.80 +0.0 2,634,294 + +0.3 2,634,295 - -0.4 2,634,331 + +0.9 2,634,386 - CA265_RS10660 0.20 +2.8 2,634,389 + CA265_RS10660 0.21 +1.5 2,634,624 - CA265_RS10660 0.72 +1.2 2,634,638 + CA265_RS10660 0.75 +1.3 2,634,682 + CA265_RS10660 0.84 +2.5 2,634,717 + +1.5 2,634,743 - +2.4 2,634,764 - -0.4 2,634,812 + +0.9 2,634,812 + +1.3 2,634,818 + -0.5 2,634,819 - +0.5 2,635,008 + CA265_RS10665 0.30 +0.2 2,635,015 - CA265_RS10665 0.31 +1.2 2,635,015 - CA265_RS10665 0.31 -0.3 2,635,017 + CA265_RS10665 0.32 +0.8 2,635,017 + CA265_RS10665 0.32 +0.7 2,635,032 - CA265_RS10665 0.34 +0.1 2,635,101 + CA265_RS10665 0.46 -0.9 2,635,101 + CA265_RS10665 0.46 +0.1 2,635,102 - CA265_RS10665 0.46 -0.4 2,635,224 + CA265_RS10665 0.67 +0.0 2,635,225 - CA265_RS10665 0.67 +0.4 2,635,255 - CA265_RS10665 0.72 +0.3 2,635,323 + CA265_RS10665 0.83 -0.1 2,635,348 - CA265_RS10665 0.88 +1.2 2,635,415 + +2.1 2,635,487 - +0.1 2,635,493 - +0.7 2,635,537 - +0.1 2,635,557 - +1.5 2,635,563 + +0.2 2,635,602 + -0.1 2,635,682 + +0.2 2,635,746 + -0.2 2,635,777 - +0.3 2,635,807 + -0.7 2,635,808 - -0.0 2,635,834 + +0.1 2,635,864 + +0.1 2,635,864 + +0.1 2,635,935 + -0.1 2,635,935 + +0.1 2,635,983 + +0.5 2,635,984 - +1.4 2,636,005 - +1.0 2,636,008 - +1.0 2,636,022 - +1.4 2,636,071 + CA265_RS10670 0.10 -0.1 2,636,096 + CA265_RS10670 0.11 +1.3 2,636,096 + CA265_RS10670 0.11 +0.4 2,636,142 + CA265_RS10670 0.13 +0.5 2,636,157 - CA265_RS10670 0.14 +2.1 2,636,174 + CA265_RS10670 0.14 +0.0 2,636,264 + CA265_RS10670 0.18 +0.4 2,636,264 + CA265_RS10670 0.18 -0.0 2,636,264 + CA265_RS10670 0.18 -0.6 2,636,272 - CA265_RS10670 0.18 +0.0 2,636,293 - CA265_RS10670 0.19 +1.5
Or see this region's nucleotide sequence