Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10595 and CA265_RS10600 are separated by 160 nucleotides CA265_RS10600 and CA265_RS10605 overlap by 14 nucleotides CA265_RS10605 and CA265_RS10610 are separated by 158 nucleotides
CA265_RS10595: CA265_RS10595 - dicarboxylate/amino acid:cation symporter, at 2,620,969 to 2,622,345
_RS10595
CA265_RS10600: CA265_RS10600 - RNA polymerase subunit sigma-24, at 2,622,506 to 2,623,003
_RS10600
CA265_RS10605: CA265_RS10605 - hypothetical protein, at 2,622,990 to 2,623,589
_RS10605
CA265_RS10610: CA265_RS10610 - hypothetical protein, at 2,623,748 to 2,624,275
_RS10610
Position (kb)
2622
2623
2624 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2621.530 kb on + strand, within CA265_RS10595 at 2621.532 kb on + strand, within CA265_RS10595 at 2621.533 kb on - strand, within CA265_RS10595 at 2621.538 kb on - strand, within CA265_RS10595 at 2621.546 kb on - strand, within CA265_RS10595 at 2621.549 kb on - strand, within CA265_RS10595 at 2621.555 kb on - strand, within CA265_RS10595 at 2621.621 kb on + strand, within CA265_RS10595 at 2621.622 kb on - strand, within CA265_RS10595 at 2621.684 kb on - strand, within CA265_RS10595 at 2621.855 kb on - strand, within CA265_RS10595 at 2621.979 kb on + strand, within CA265_RS10595 at 2622.049 kb on + strand, within CA265_RS10595 at 2622.163 kb on - strand, within CA265_RS10595 at 2622.239 kb on - strand at 2622.239 kb on - strand at 2622.241 kb on - strand at 2622.385 kb on - strand at 2622.390 kb on - strand at 2622.482 kb on + strand at 2622.493 kb on + strand at 2622.493 kb on + strand at 2622.495 kb on + strand at 2622.495 kb on + strand at 2622.525 kb on + strand at 2622.525 kb on + strand at 2622.570 kb on + strand, within CA265_RS10600 at 2622.581 kb on + strand, within CA265_RS10600 at 2622.583 kb on + strand, within CA265_RS10600 at 2622.715 kb on - strand, within CA265_RS10600 at 2622.719 kb on + strand, within CA265_RS10600 at 2622.719 kb on + strand, within CA265_RS10600 at 2622.756 kb on + strand, within CA265_RS10600 at 2622.763 kb on + strand, within CA265_RS10600 at 2622.783 kb on + strand, within CA265_RS10600 at 2622.826 kb on + strand, within CA265_RS10600 at 2622.869 kb on - strand, within CA265_RS10600 at 2622.893 kb on + strand, within CA265_RS10600 at 2622.959 kb on - strand at 2622.959 kb on - strand at 2622.978 kb on + strand at 2622.979 kb on - strand at 2623.594 kb on - strand at 2623.665 kb on + strand at 2623.874 kb on + strand, within CA265_RS10610 at 2623.875 kb on - strand, within CA265_RS10610 at 2623.875 kb on - strand, within CA265_RS10610 at 2623.876 kb on + strand, within CA265_RS10610 at 2623.877 kb on - strand, within CA265_RS10610 at 2623.877 kb on - strand, within CA265_RS10610 at 2623.877 kb on - strand at 2623.880 kb on + strand, within CA265_RS10610 at 2623.881 kb on - strand, within CA265_RS10610 at 2623.915 kb on + strand, within CA265_RS10610 at 2623.916 kb on - strand, within CA265_RS10610 at 2623.916 kb on - strand, within CA265_RS10610 at 2623.933 kb on - strand, within CA265_RS10610 at 2623.959 kb on + strand, within CA265_RS10610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 2,621,530 + CA265_RS10595 0.41 +1.8 2,621,532 + CA265_RS10595 0.41 +2.4 2,621,533 - CA265_RS10595 0.41 +1.6 2,621,538 - CA265_RS10595 0.41 +1.2 2,621,546 - CA265_RS10595 0.42 +0.7 2,621,549 - CA265_RS10595 0.42 -0.5 2,621,555 - CA265_RS10595 0.43 +1.1 2,621,621 + CA265_RS10595 0.47 +2.5 2,621,622 - CA265_RS10595 0.47 +0.7 2,621,684 - CA265_RS10595 0.52 +0.1 2,621,855 - CA265_RS10595 0.64 +0.6 2,621,979 + CA265_RS10595 0.73 +5.0 2,622,049 + CA265_RS10595 0.78 +1.8 2,622,163 - CA265_RS10595 0.87 -0.8 2,622,239 - +0.8 2,622,239 - +1.4 2,622,241 - -0.2 2,622,385 - -1.7 2,622,390 - -1.1 2,622,482 + +0.0 2,622,493 + -1.1 2,622,493 + -0.6 2,622,495 + -0.7 2,622,495 + -1.4 2,622,525 + -0.5 2,622,525 + -1.2 2,622,570 + CA265_RS10600 0.13 -1.5 2,622,581 + CA265_RS10600 0.15 -1.6 2,622,583 + CA265_RS10600 0.15 -0.3 2,622,715 - CA265_RS10600 0.42 -0.2 2,622,719 + CA265_RS10600 0.43 -1.1 2,622,719 + CA265_RS10600 0.43 -1.6 2,622,756 + CA265_RS10600 0.50 -0.7 2,622,763 + CA265_RS10600 0.52 -1.1 2,622,783 + CA265_RS10600 0.56 -0.1 2,622,826 + CA265_RS10600 0.64 -1.4 2,622,869 - CA265_RS10600 0.73 -1.4 2,622,893 + CA265_RS10600 0.78 -1.7 2,622,959 - -2.0 2,622,959 - -1.1 2,622,978 + -0.5 2,622,979 - -1.4 2,623,594 - -2.6 2,623,665 + -0.2 2,623,874 + CA265_RS10610 0.24 +0.6 2,623,875 - CA265_RS10610 0.24 -1.3 2,623,875 - CA265_RS10610 0.24 -1.2 2,623,876 + CA265_RS10610 0.24 +0.3 2,623,877 - CA265_RS10610 0.24 -1.0 2,623,877 - CA265_RS10610 0.24 +0.5 2,623,877 - -0.5 2,623,880 + CA265_RS10610 0.25 +0.6 2,623,881 - CA265_RS10610 0.25 -2.2 2,623,915 + CA265_RS10610 0.32 -0.2 2,623,916 - CA265_RS10610 0.32 -0.4 2,623,916 - CA265_RS10610 0.32 -0.0 2,623,933 - CA265_RS10610 0.35 -1.4 2,623,959 + CA265_RS10610 0.40 +1.1
Or see this region's nucleotide sequence