Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10405 and CA265_RS10410 are separated by 174 nucleotides CA265_RS10410 and CA265_RS10415 are separated by 117 nucleotides CA265_RS10415 and CA265_RS10420 are separated by 95 nucleotides
CA265_RS10405: CA265_RS10405 - hypothetical protein, at 2,574,961 to 2,576,172
_RS10405
CA265_RS10410: CA265_RS10410 - hypothetical protein, at 2,576,347 to 2,576,958
_RS10410
CA265_RS10415: CA265_RS10415 - type I methionyl aminopeptidase, at 2,577,076 to 2,577,876
_RS10415
CA265_RS10420: CA265_RS10420 - hydroxyacid dehydrogenase, at 2,577,972 to 2,578,976
_RS10420
Position (kb)
2577
2578 Strain fitness (log2 ratio)
-1
0
1
2 at 2576.163 kb on - strand at 2576.170 kb on + strand at 2576.171 kb on - strand at 2576.178 kb on + strand at 2576.281 kb on + strand at 2576.281 kb on + strand at 2576.281 kb on + strand at 2576.288 kb on - strand at 2576.288 kb on - strand at 2576.312 kb on + strand at 2576.346 kb on + strand at 2576.347 kb on - strand at 2576.347 kb on - strand at 2576.446 kb on - strand, within CA265_RS10410 at 2576.476 kb on - strand, within CA265_RS10410 at 2576.476 kb on - strand, within CA265_RS10410 at 2576.496 kb on + strand, within CA265_RS10410 at 2576.544 kb on - strand, within CA265_RS10410 at 2576.597 kb on + strand, within CA265_RS10410 at 2576.606 kb on + strand, within CA265_RS10410 at 2576.606 kb on + strand, within CA265_RS10410 at 2576.644 kb on + strand, within CA265_RS10410 at 2576.680 kb on - strand, within CA265_RS10410 at 2576.687 kb on - strand, within CA265_RS10410 at 2576.869 kb on + strand, within CA265_RS10410 at 2576.941 kb on + strand at 2576.997 kb on + strand at 2577.004 kb on + strand at 2577.129 kb on - strand at 2577.160 kb on - strand, within CA265_RS10415 at 2577.164 kb on + strand, within CA265_RS10415 at 2577.181 kb on - strand, within CA265_RS10415 at 2577.216 kb on + strand, within CA265_RS10415 at 2577.236 kb on + strand, within CA265_RS10415 at 2577.237 kb on - strand, within CA265_RS10415 at 2577.256 kb on - strand, within CA265_RS10415 at 2577.277 kb on - strand, within CA265_RS10415 at 2577.277 kb on - strand, within CA265_RS10415 at 2577.279 kb on + strand, within CA265_RS10415 at 2577.279 kb on - strand, within CA265_RS10415 at 2577.303 kb on - strand, within CA265_RS10415 at 2577.345 kb on + strand, within CA265_RS10415 at 2577.346 kb on - strand, within CA265_RS10415 at 2577.435 kb on - strand, within CA265_RS10415 at 2577.435 kb on - strand, within CA265_RS10415 at 2577.445 kb on - strand, within CA265_RS10415 at 2577.453 kb on + strand, within CA265_RS10415 at 2577.454 kb on - strand, within CA265_RS10415 at 2577.454 kb on - strand, within CA265_RS10415 at 2577.560 kb on + strand, within CA265_RS10415 at 2577.584 kb on + strand, within CA265_RS10415 at 2577.585 kb on - strand, within CA265_RS10415 at 2577.614 kb on + strand, within CA265_RS10415 at 2577.619 kb on - strand, within CA265_RS10415 at 2577.622 kb on + strand, within CA265_RS10415 at 2577.645 kb on - strand, within CA265_RS10415 at 2577.647 kb on + strand, within CA265_RS10415 at 2577.779 kb on + strand, within CA265_RS10415 at 2577.779 kb on + strand, within CA265_RS10415 at 2577.779 kb on + strand, within CA265_RS10415 at 2577.779 kb on + strand, within CA265_RS10415 at 2577.779 kb on + strand, within CA265_RS10415 at 2577.787 kb on - strand, within CA265_RS10415 at 2577.805 kb on - strand at 2577.885 kb on + strand at 2577.970 kb on + strand at 2577.982 kb on - strand at 2578.023 kb on + strand at 2578.023 kb on + strand at 2578.024 kb on - strand at 2578.024 kb on - strand at 2578.133 kb on + strand, within CA265_RS10420 at 2578.152 kb on - strand, within CA265_RS10420 at 2578.260 kb on - strand, within CA265_RS10420 at 2578.287 kb on + strand, within CA265_RS10420 at 2578.288 kb on - strand, within CA265_RS10420 at 2578.288 kb on - strand, within CA265_RS10420 at 2578.352 kb on + strand, within CA265_RS10420 at 2578.411 kb on + strand, within CA265_RS10420 at 2578.411 kb on + strand, within CA265_RS10420 at 2578.412 kb on - strand, within CA265_RS10420 at 2578.602 kb on - strand, within CA265_RS10420 at 2578.603 kb on + strand, within CA265_RS10420 at 2578.631 kb on + strand, within CA265_RS10420 at 2578.733 kb on + strand, within CA265_RS10420 at 2578.787 kb on + strand, within CA265_RS10420 at 2578.788 kb on - strand, within CA265_RS10420 at 2578.806 kb on - strand, within CA265_RS10420 at 2578.811 kb on + strand, within CA265_RS10420 at 2578.818 kb on + strand, within CA265_RS10420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 2,576,163 - +1.0 2,576,170 + +0.8 2,576,171 - +0.5 2,576,178 + +0.4 2,576,281 + -0.5 2,576,281 + +0.2 2,576,281 + +0.3 2,576,288 - +0.4 2,576,288 - +1.5 2,576,312 + -0.3 2,576,346 + +0.4 2,576,347 - +0.1 2,576,347 - +0.9 2,576,446 - CA265_RS10410 0.16 +0.2 2,576,476 - CA265_RS10410 0.21 -0.5 2,576,476 - CA265_RS10410 0.21 -0.3 2,576,496 + CA265_RS10410 0.24 +0.3 2,576,544 - CA265_RS10410 0.32 +0.0 2,576,597 + CA265_RS10410 0.41 +0.0 2,576,606 + CA265_RS10410 0.42 +0.2 2,576,606 + CA265_RS10410 0.42 +0.1 2,576,644 + CA265_RS10410 0.49 +0.3 2,576,680 - CA265_RS10410 0.54 -0.4 2,576,687 - CA265_RS10410 0.56 +0.5 2,576,869 + CA265_RS10410 0.85 +0.2 2,576,941 + -0.2 2,576,997 + +0.6 2,577,004 + +0.4 2,577,129 - +0.3 2,577,160 - CA265_RS10415 0.10 +1.1 2,577,164 + CA265_RS10415 0.11 +0.2 2,577,181 - CA265_RS10415 0.13 -0.2 2,577,216 + CA265_RS10415 0.17 +0.6 2,577,236 + CA265_RS10415 0.20 +1.2 2,577,237 - CA265_RS10415 0.20 +0.4 2,577,256 - CA265_RS10415 0.22 +0.1 2,577,277 - CA265_RS10415 0.25 +0.5 2,577,277 - CA265_RS10415 0.25 +1.2 2,577,279 + CA265_RS10415 0.25 +0.8 2,577,279 - CA265_RS10415 0.25 +0.2 2,577,303 - CA265_RS10415 0.28 +0.8 2,577,345 + CA265_RS10415 0.34 +1.5 2,577,346 - CA265_RS10415 0.34 +0.1 2,577,435 - CA265_RS10415 0.45 +1.1 2,577,435 - CA265_RS10415 0.45 +1.2 2,577,445 - CA265_RS10415 0.46 +2.7 2,577,453 + CA265_RS10415 0.47 +0.4 2,577,454 - CA265_RS10415 0.47 -0.1 2,577,454 - CA265_RS10415 0.47 +0.3 2,577,560 + CA265_RS10415 0.60 -0.2 2,577,584 + CA265_RS10415 0.63 +2.5 2,577,585 - CA265_RS10415 0.64 -0.2 2,577,614 + CA265_RS10415 0.67 +1.2 2,577,619 - CA265_RS10415 0.68 +0.7 2,577,622 + CA265_RS10415 0.68 +1.2 2,577,645 - CA265_RS10415 0.71 -0.2 2,577,647 + CA265_RS10415 0.71 +0.3 2,577,779 + CA265_RS10415 0.88 +0.4 2,577,779 + CA265_RS10415 0.88 +0.7 2,577,779 + CA265_RS10415 0.88 +2.7 2,577,779 + CA265_RS10415 0.88 +0.3 2,577,779 + CA265_RS10415 0.88 +0.7 2,577,787 - CA265_RS10415 0.89 +1.2 2,577,805 - +1.0 2,577,885 + +0.7 2,577,970 + -0.4 2,577,982 - +1.0 2,578,023 + -0.1 2,578,023 + -0.1 2,578,024 - -1.0 2,578,024 - +0.2 2,578,133 + CA265_RS10420 0.16 +0.2 2,578,152 - CA265_RS10420 0.18 +1.2 2,578,260 - CA265_RS10420 0.29 +0.3 2,578,287 + CA265_RS10420 0.31 -1.2 2,578,288 - CA265_RS10420 0.31 +0.3 2,578,288 - CA265_RS10420 0.31 -0.6 2,578,352 + CA265_RS10420 0.38 -1.1 2,578,411 + CA265_RS10420 0.44 -0.1 2,578,411 + CA265_RS10420 0.44 -0.1 2,578,412 - CA265_RS10420 0.44 +0.4 2,578,602 - CA265_RS10420 0.63 +0.4 2,578,603 + CA265_RS10420 0.63 -0.4 2,578,631 + CA265_RS10420 0.66 -0.4 2,578,733 + CA265_RS10420 0.76 +0.1 2,578,787 + CA265_RS10420 0.81 +0.2 2,578,788 - CA265_RS10420 0.81 +0.0 2,578,806 - CA265_RS10420 0.83 +0.9 2,578,811 + CA265_RS10420 0.83 -0.3 2,578,818 + CA265_RS10420 0.84 +0.8
Or see this region's nucleotide sequence