Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09385 and CA265_RS09390 overlap by 1 nucleotides CA265_RS09390 and CA265_RS09395 are separated by 57 nucleotides CA265_RS09395 and CA265_RS09400 are separated by 92 nucleotides CA265_RS09400 and CA265_RS09405 are separated by 3 nucleotides
CA265_RS09385: CA265_RS09385 - hypothetical protein, at 2,329,762 to 2,330,211
_RS09385
CA265_RS09390: CA265_RS09390 - hypothetical protein, at 2,330,211 to 2,330,741
_RS09390
CA265_RS09395: CA265_RS09395 - hypothetical protein, at 2,330,799 to 2,331,269
_RS09395
CA265_RS09400: CA265_RS09400 - hypothetical protein, at 2,331,362 to 2,331,841
_RS09400
CA265_RS09405: CA265_RS09405 - hypothetical protein, at 2,331,845 to 2,332,804
_RS09405
Position (kb)
2330
2331
2332 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2329.821 kb on + strand, within CA265_RS09385 at 2329.821 kb on + strand, within CA265_RS09385 at 2329.878 kb on - strand, within CA265_RS09385 at 2329.892 kb on - strand, within CA265_RS09385 at 2329.915 kb on - strand, within CA265_RS09385 at 2329.944 kb on + strand, within CA265_RS09385 at 2329.953 kb on + strand, within CA265_RS09385 at 2330.036 kb on - strand, within CA265_RS09385 at 2330.036 kb on - strand, within CA265_RS09385 at 2330.131 kb on + strand, within CA265_RS09385 at 2330.135 kb on + strand, within CA265_RS09385 at 2330.213 kb on - strand at 2330.225 kb on + strand at 2330.225 kb on + strand at 2330.226 kb on - strand at 2330.235 kb on - strand at 2330.245 kb on + strand at 2330.246 kb on - strand at 2330.251 kb on - strand at 2330.262 kb on - strand at 2330.285 kb on + strand, within CA265_RS09390 at 2330.347 kb on - strand, within CA265_RS09390 at 2330.351 kb on + strand, within CA265_RS09390 at 2330.381 kb on + strand, within CA265_RS09390 at 2330.423 kb on + strand, within CA265_RS09390 at 2330.425 kb on + strand, within CA265_RS09390 at 2330.425 kb on + strand, within CA265_RS09390 at 2330.425 kb on + strand, within CA265_RS09390 at 2330.426 kb on - strand, within CA265_RS09390 at 2330.426 kb on - strand, within CA265_RS09390 at 2330.426 kb on - strand, within CA265_RS09390 at 2330.467 kb on + strand, within CA265_RS09390 at 2330.468 kb on - strand, within CA265_RS09390 at 2330.514 kb on + strand, within CA265_RS09390 at 2330.565 kb on + strand, within CA265_RS09390 at 2330.566 kb on - strand, within CA265_RS09390 at 2330.644 kb on - strand, within CA265_RS09390 at 2330.658 kb on + strand, within CA265_RS09390 at 2330.659 kb on - strand, within CA265_RS09390 at 2330.735 kb on + strand at 2330.899 kb on + strand, within CA265_RS09395 at 2330.899 kb on + strand, within CA265_RS09395 at 2330.980 kb on - strand, within CA265_RS09395 at 2331.011 kb on + strand, within CA265_RS09395 at 2331.015 kb on - strand, within CA265_RS09395 at 2331.048 kb on - strand, within CA265_RS09395 at 2331.186 kb on - strand, within CA265_RS09395 at 2331.235 kb on + strand at 2331.407 kb on - strand at 2331.433 kb on + strand, within CA265_RS09400 at 2331.527 kb on - strand, within CA265_RS09400 at 2331.579 kb on + strand, within CA265_RS09400 at 2331.580 kb on - strand, within CA265_RS09400 at 2331.600 kb on + strand, within CA265_RS09400 at 2331.600 kb on + strand, within CA265_RS09400 at 2331.632 kb on - strand, within CA265_RS09400 at 2331.673 kb on + strand, within CA265_RS09400 at 2331.801 kb on - strand at 2331.920 kb on - strand at 2331.954 kb on + strand, within CA265_RS09405 at 2331.955 kb on - strand, within CA265_RS09405 at 2331.963 kb on + strand, within CA265_RS09405 at 2331.964 kb on - strand, within CA265_RS09405 at 2331.973 kb on + strand, within CA265_RS09405 at 2331.974 kb on - strand, within CA265_RS09405 at 2332.072 kb on + strand, within CA265_RS09405 at 2332.078 kb on + strand, within CA265_RS09405 at 2332.082 kb on - strand, within CA265_RS09405 at 2332.099 kb on - strand, within CA265_RS09405 at 2332.116 kb on + strand, within CA265_RS09405 at 2332.145 kb on + strand, within CA265_RS09405 at 2332.162 kb on + strand, within CA265_RS09405 at 2332.258 kb on + strand, within CA265_RS09405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 2,329,821 + CA265_RS09385 0.13 +0.4 2,329,821 + CA265_RS09385 0.13 -1.1 2,329,878 - CA265_RS09385 0.26 -2.0 2,329,892 - CA265_RS09385 0.29 -1.6 2,329,915 - CA265_RS09385 0.34 -1.2 2,329,944 + CA265_RS09385 0.40 +0.4 2,329,953 + CA265_RS09385 0.42 +0.2 2,330,036 - CA265_RS09385 0.61 -1.7 2,330,036 - CA265_RS09385 0.61 -0.5 2,330,131 + CA265_RS09385 0.82 -0.3 2,330,135 + CA265_RS09385 0.83 +0.3 2,330,213 - -0.5 2,330,225 + -0.2 2,330,225 + +1.6 2,330,226 - -1.5 2,330,235 - -2.0 2,330,245 + -0.2 2,330,246 - -2.0 2,330,251 - -1.3 2,330,262 - -1.9 2,330,285 + CA265_RS09390 0.14 -0.9 2,330,347 - CA265_RS09390 0.26 -3.2 2,330,351 + CA265_RS09390 0.26 +0.4 2,330,381 + CA265_RS09390 0.32 +0.3 2,330,423 + CA265_RS09390 0.40 +0.6 2,330,425 + CA265_RS09390 0.40 -0.3 2,330,425 + CA265_RS09390 0.40 +0.4 2,330,425 + CA265_RS09390 0.40 -0.6 2,330,426 - CA265_RS09390 0.40 -0.8 2,330,426 - CA265_RS09390 0.40 -1.4 2,330,426 - CA265_RS09390 0.40 -2.4 2,330,467 + CA265_RS09390 0.48 +0.8 2,330,468 - CA265_RS09390 0.48 -2.9 2,330,514 + CA265_RS09390 0.57 +0.2 2,330,565 + CA265_RS09390 0.67 +0.4 2,330,566 - CA265_RS09390 0.67 -1.1 2,330,644 - CA265_RS09390 0.82 -1.3 2,330,658 + CA265_RS09390 0.84 +1.1 2,330,659 - CA265_RS09390 0.84 -0.5 2,330,735 + +0.6 2,330,899 + CA265_RS09395 0.21 +0.3 2,330,899 + CA265_RS09395 0.21 -1.3 2,330,980 - CA265_RS09395 0.38 -1.7 2,331,011 + CA265_RS09395 0.45 +0.2 2,331,015 - CA265_RS09395 0.46 -0.6 2,331,048 - CA265_RS09395 0.53 -0.2 2,331,186 - CA265_RS09395 0.82 +0.2 2,331,235 + -0.1 2,331,407 - +2.3 2,331,433 + CA265_RS09400 0.15 -0.9 2,331,527 - CA265_RS09400 0.34 +4.3 2,331,579 + CA265_RS09400 0.45 -0.0 2,331,580 - CA265_RS09400 0.45 +3.1 2,331,600 + CA265_RS09400 0.50 +0.2 2,331,600 + CA265_RS09400 0.50 +0.3 2,331,632 - CA265_RS09400 0.56 +3.6 2,331,673 + CA265_RS09400 0.65 -0.5 2,331,801 - +2.7 2,331,920 - +4.3 2,331,954 + CA265_RS09405 0.11 -0.8 2,331,955 - CA265_RS09405 0.11 +2.6 2,331,963 + CA265_RS09405 0.12 +0.1 2,331,964 - CA265_RS09405 0.12 +3.4 2,331,973 + CA265_RS09405 0.13 +0.1 2,331,974 - CA265_RS09405 0.13 +4.1 2,332,072 + CA265_RS09405 0.24 -0.8 2,332,078 + CA265_RS09405 0.24 +0.1 2,332,082 - CA265_RS09405 0.25 +2.0 2,332,099 - CA265_RS09405 0.26 +0.5 2,332,116 + CA265_RS09405 0.28 +0.2 2,332,145 + CA265_RS09405 0.31 +0.5 2,332,162 + CA265_RS09405 0.33 -0.7 2,332,258 + CA265_RS09405 0.43 +0.1
Or see this region's nucleotide sequence