Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08930 and CA265_RS08935 are separated by 107 nucleotides CA265_RS08935 and CA265_RS08940 are separated by 61 nucleotides CA265_RS08940 and CA265_RS08945 are separated by 3 nucleotides CA265_RS08945 and CA265_RS08950 are separated by 153 nucleotides
CA265_RS08930: CA265_RS08930 - 7-carboxy-7-deazaguanine synthase QueE, at 2,230,973 to 2,231,596
_RS08930
CA265_RS08935: CA265_RS08935 - (2Fe-2S)-binding protein, at 2,231,704 to 2,232,039
_RS08935
CA265_RS08940: CA265_RS08940 - twitching motility protein PilT, at 2,232,101 to 2,232,520
_RS08940
CA265_RS08945: CA265_RS08945 - hypothetical protein, at 2,232,524 to 2,232,784
_RS08945
CA265_RS08950: CA265_RS08950 - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase, at 2,232,938 to 2,233,858
_RS08950
Position (kb)
2232
2233 Strain fitness (log2 ratio)
-1
0
1
2 at 2231.534 kb on + strand at 2231.539 kb on + strand at 2231.565 kb on - strand at 2231.565 kb on - strand at 2231.583 kb on - strand at 2231.684 kb on - strand at 2231.692 kb on + strand at 2231.723 kb on + strand at 2231.728 kb on - strand at 2231.728 kb on - strand at 2231.809 kb on - strand, within CA265_RS08935 at 2231.913 kb on + strand, within CA265_RS08935 at 2231.913 kb on + strand, within CA265_RS08935 at 2231.913 kb on + strand, within CA265_RS08935 at 2231.953 kb on + strand, within CA265_RS08935 at 2231.953 kb on + strand, within CA265_RS08935 at 2231.954 kb on - strand, within CA265_RS08935 at 2231.995 kb on + strand, within CA265_RS08935 at 2231.996 kb on - strand, within CA265_RS08935 at 2231.996 kb on - strand, within CA265_RS08935 at 2232.173 kb on + strand, within CA265_RS08940 at 2232.174 kb on - strand, within CA265_RS08940 at 2232.175 kb on + strand, within CA265_RS08940 at 2232.196 kb on + strand, within CA265_RS08940 at 2232.196 kb on + strand, within CA265_RS08940 at 2232.196 kb on + strand, within CA265_RS08940 at 2232.196 kb on + strand, within CA265_RS08940 at 2232.197 kb on - strand, within CA265_RS08940 at 2232.197 kb on - strand, within CA265_RS08940 at 2232.197 kb on - strand, within CA265_RS08940 at 2232.197 kb on - strand, within CA265_RS08940 at 2232.199 kb on + strand, within CA265_RS08940 at 2232.199 kb on + strand, within CA265_RS08940 at 2232.220 kb on - strand, within CA265_RS08940 at 2232.222 kb on + strand, within CA265_RS08940 at 2232.304 kb on + strand, within CA265_RS08940 at 2232.305 kb on - strand, within CA265_RS08940 at 2232.405 kb on + strand, within CA265_RS08940 at 2232.405 kb on + strand, within CA265_RS08940 at 2232.407 kb on + strand at 2232.407 kb on + strand, within CA265_RS08940 at 2232.407 kb on + strand, within CA265_RS08940 at 2232.432 kb on - strand, within CA265_RS08940 at 2232.454 kb on - strand, within CA265_RS08940 at 2232.476 kb on - strand, within CA265_RS08940 at 2232.507 kb on - strand at 2232.562 kb on + strand, within CA265_RS08945 at 2232.594 kb on - strand, within CA265_RS08945 at 2232.614 kb on - strand, within CA265_RS08945 at 2232.617 kb on + strand, within CA265_RS08945 at 2232.654 kb on + strand, within CA265_RS08945 at 2232.677 kb on + strand, within CA265_RS08945 at 2232.689 kb on + strand, within CA265_RS08945 at 2232.701 kb on + strand, within CA265_RS08945 at 2232.707 kb on - strand, within CA265_RS08945 at 2232.707 kb on - strand, within CA265_RS08945 at 2232.765 kb on - strand at 2232.930 kb on + strand at 2232.945 kb on + strand at 2232.945 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 2,231,534 + -0.2 2,231,539 + +0.5 2,231,565 - -0.1 2,231,565 - -0.9 2,231,583 - -0.7 2,231,684 - -0.7 2,231,692 + -0.1 2,231,723 + -0.3 2,231,728 - +0.4 2,231,728 - -0.1 2,231,809 - CA265_RS08935 0.31 -0.4 2,231,913 + CA265_RS08935 0.62 +0.1 2,231,913 + CA265_RS08935 0.62 -0.4 2,231,913 + CA265_RS08935 0.62 -0.0 2,231,953 + CA265_RS08935 0.74 +0.6 2,231,953 + CA265_RS08935 0.74 +0.2 2,231,954 - CA265_RS08935 0.74 -0.2 2,231,995 + CA265_RS08935 0.87 +0.7 2,231,996 - CA265_RS08935 0.87 +0.7 2,231,996 - CA265_RS08935 0.87 -0.4 2,232,173 + CA265_RS08940 0.17 -0.4 2,232,174 - CA265_RS08940 0.17 -0.5 2,232,175 + CA265_RS08940 0.18 -0.6 2,232,196 + CA265_RS08940 0.23 +0.1 2,232,196 + CA265_RS08940 0.23 -0.2 2,232,196 + CA265_RS08940 0.23 -0.1 2,232,196 + CA265_RS08940 0.23 -0.7 2,232,197 - CA265_RS08940 0.23 +0.9 2,232,197 - CA265_RS08940 0.23 +0.1 2,232,197 - CA265_RS08940 0.23 +0.4 2,232,197 - CA265_RS08940 0.23 -0.1 2,232,199 + CA265_RS08940 0.23 -0.4 2,232,199 + CA265_RS08940 0.23 +0.6 2,232,220 - CA265_RS08940 0.28 -1.7 2,232,222 + CA265_RS08940 0.29 +1.0 2,232,304 + CA265_RS08940 0.48 +0.3 2,232,305 - CA265_RS08940 0.49 -0.3 2,232,405 + CA265_RS08940 0.72 +0.2 2,232,405 + CA265_RS08940 0.72 -0.8 2,232,407 + +1.9 2,232,407 + CA265_RS08940 0.73 -0.2 2,232,407 + CA265_RS08940 0.73 +0.0 2,232,432 - CA265_RS08940 0.79 -0.3 2,232,454 - CA265_RS08940 0.84 +0.1 2,232,476 - CA265_RS08940 0.89 +1.0 2,232,507 - +0.5 2,232,562 + CA265_RS08945 0.15 -1.2 2,232,594 - CA265_RS08945 0.27 -1.0 2,232,614 - CA265_RS08945 0.34 -0.3 2,232,617 + CA265_RS08945 0.36 -1.0 2,232,654 + CA265_RS08945 0.50 -0.3 2,232,677 + CA265_RS08945 0.59 -0.5 2,232,689 + CA265_RS08945 0.63 -0.9 2,232,701 + CA265_RS08945 0.68 -0.4 2,232,707 - CA265_RS08945 0.70 -0.3 2,232,707 - CA265_RS08945 0.70 -0.5 2,232,765 - -0.6 2,232,930 + +0.3 2,232,945 + -0.0 2,232,945 + +0.1
Or see this region's nucleotide sequence