Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07325 and CA265_RS07330 are separated by 305 nucleotides CA265_RS07330 and CA265_RS07335 are separated by 256 nucleotides CA265_RS07335 and CA265_RS07340 are separated by 76 nucleotides
CA265_RS07325: CA265_RS07325 - phytanoyl-CoA dioxygenase family protein, at 1,847,402 to 1,848,298
_RS07325
CA265_RS07330: CA265_RS07330 - hypothetical protein, at 1,848,604 to 1,848,966
_RS07330
CA265_RS07335: CA265_RS07335 - hypothetical protein, at 1,849,223 to 1,849,615
_RS07335
CA265_RS07340: CA265_RS07340 - hypothetical protein, at 1,849,692 to 1,850,852
_RS07340
Position (kb)
1848
1849 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1847.608 kb on + strand, within CA265_RS07325 at 1847.613 kb on + strand, within CA265_RS07325 at 1847.674 kb on - strand, within CA265_RS07325 at 1847.702 kb on + strand, within CA265_RS07325 at 1847.714 kb on - strand, within CA265_RS07325 at 1847.748 kb on + strand, within CA265_RS07325 at 1847.749 kb on - strand, within CA265_RS07325 at 1847.751 kb on + strand, within CA265_RS07325 at 1847.839 kb on - strand, within CA265_RS07325 at 1847.839 kb on - strand, within CA265_RS07325 at 1847.917 kb on + strand, within CA265_RS07325 at 1847.918 kb on - strand, within CA265_RS07325 at 1847.967 kb on - strand, within CA265_RS07325 at 1848.072 kb on - strand, within CA265_RS07325 at 1848.072 kb on - strand, within CA265_RS07325 at 1848.140 kb on + strand, within CA265_RS07325 at 1848.196 kb on + strand, within CA265_RS07325 at 1848.204 kb on + strand, within CA265_RS07325 at 1848.205 kb on - strand, within CA265_RS07325 at 1848.315 kb on - strand at 1848.365 kb on + strand at 1848.431 kb on - strand at 1848.447 kb on + strand at 1848.448 kb on - strand at 1848.453 kb on - strand at 1848.486 kb on - strand at 1848.531 kb on + strand at 1848.543 kb on - strand at 1848.546 kb on + strand at 1848.549 kb on - strand at 1848.555 kb on - strand at 1848.600 kb on + strand at 1848.721 kb on + strand, within CA265_RS07330 at 1848.722 kb on - strand, within CA265_RS07330 at 1848.722 kb on - strand, within CA265_RS07330 at 1848.868 kb on - strand, within CA265_RS07330 at 1848.875 kb on - strand, within CA265_RS07330 at 1848.934 kb on + strand at 1848.973 kb on - strand at 1848.990 kb on + strand at 1848.990 kb on + strand at 1848.991 kb on - strand at 1849.039 kb on - strand at 1849.071 kb on + strand at 1849.071 kb on + strand at 1849.078 kb on - strand at 1849.134 kb on - strand at 1849.145 kb on - strand at 1849.150 kb on + strand at 1849.171 kb on + strand at 1849.185 kb on + strand at 1849.220 kb on - strand at 1849.324 kb on + strand, within CA265_RS07335 at 1849.324 kb on + strand, within CA265_RS07335 at 1849.371 kb on - strand, within CA265_RS07335 at 1849.393 kb on + strand, within CA265_RS07335 at 1849.519 kb on + strand, within CA265_RS07335 at 1849.547 kb on - strand, within CA265_RS07335 at 1849.597 kb on + strand at 1849.613 kb on + strand at 1849.634 kb on - strand at 1849.637 kb on + strand at 1849.641 kb on + strand at 1849.757 kb on + strand at 1849.757 kb on + strand at 1849.758 kb on - strand at 1849.758 kb on - strand at 1849.770 kb on + strand at 1849.778 kb on + strand at 1849.792 kb on - strand at 1849.795 kb on + strand at 1849.796 kb on - strand at 1849.797 kb on + strand at 1849.821 kb on - strand, within CA265_RS07340 at 1849.889 kb on + strand, within CA265_RS07340 at 1849.890 kb on - strand, within CA265_RS07340 at 1849.920 kb on - strand, within CA265_RS07340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 1,847,608 + CA265_RS07325 0.23 -1.0 1,847,613 + CA265_RS07325 0.24 -0.2 1,847,674 - CA265_RS07325 0.30 +0.8 1,847,702 + CA265_RS07325 0.33 +0.2 1,847,714 - CA265_RS07325 0.35 +0.5 1,847,748 + CA265_RS07325 0.39 -0.1 1,847,749 - CA265_RS07325 0.39 -0.3 1,847,751 + CA265_RS07325 0.39 +0.2 1,847,839 - CA265_RS07325 0.49 -0.4 1,847,839 - CA265_RS07325 0.49 +0.7 1,847,917 + CA265_RS07325 0.57 +0.3 1,847,918 - CA265_RS07325 0.58 +0.5 1,847,967 - CA265_RS07325 0.63 +1.5 1,848,072 - CA265_RS07325 0.75 +2.1 1,848,072 - CA265_RS07325 0.75 +0.6 1,848,140 + CA265_RS07325 0.82 -0.1 1,848,196 + CA265_RS07325 0.89 -0.4 1,848,204 + CA265_RS07325 0.89 -0.5 1,848,205 - CA265_RS07325 0.90 +0.1 1,848,315 - -0.8 1,848,365 + -0.3 1,848,431 - +0.3 1,848,447 + +0.1 1,848,448 - +0.1 1,848,453 - -0.9 1,848,486 - +0.3 1,848,531 + -0.2 1,848,543 - +0.9 1,848,546 + +0.7 1,848,549 - -0.0 1,848,555 - +0.8 1,848,600 + -0.2 1,848,721 + CA265_RS07330 0.32 -0.3 1,848,722 - CA265_RS07330 0.33 -0.5 1,848,722 - CA265_RS07330 0.33 +0.4 1,848,868 - CA265_RS07330 0.73 +1.0 1,848,875 - CA265_RS07330 0.75 +1.0 1,848,934 + -0.1 1,848,973 - -0.8 1,848,990 + +0.5 1,848,990 + -0.7 1,848,991 - -0.6 1,849,039 - -1.8 1,849,071 + -0.1 1,849,071 + +0.4 1,849,078 - -0.1 1,849,134 - +0.8 1,849,145 - +0.1 1,849,150 + -0.3 1,849,171 + +0.9 1,849,185 + +1.0 1,849,220 - -1.3 1,849,324 + CA265_RS07335 0.26 -0.0 1,849,324 + CA265_RS07335 0.26 -0.4 1,849,371 - CA265_RS07335 0.38 +0.6 1,849,393 + CA265_RS07335 0.43 -0.1 1,849,519 + CA265_RS07335 0.75 +1.4 1,849,547 - CA265_RS07335 0.82 +0.2 1,849,597 + -0.4 1,849,613 + +0.2 1,849,634 - +0.6 1,849,637 + -0.2 1,849,641 + +0.4 1,849,757 + -0.1 1,849,757 + +0.8 1,849,758 - -1.5 1,849,758 - +0.0 1,849,770 + +0.3 1,849,778 + -0.1 1,849,792 - +0.5 1,849,795 + -0.3 1,849,796 - -0.0 1,849,797 + -1.0 1,849,821 - CA265_RS07340 0.11 -0.3 1,849,889 + CA265_RS07340 0.17 +0.1 1,849,890 - CA265_RS07340 0.17 -0.0 1,849,920 - CA265_RS07340 0.20 +0.2
Or see this region's nucleotide sequence