Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03680 and CA265_RS03685 are separated by 2 nucleotides CA265_RS03685 and CA265_RS03690 are separated by 24 nucleotides CA265_RS03690 and CA265_RS03695 are separated by 58 nucleotides CA265_RS03695 and CA265_RS03700 are separated by 40 nucleotides
CA265_RS03680: CA265_RS03680 - RNA 2'-phosphotransferase, at 876,685 to 877,227
_RS03680
CA265_RS03685: CA265_RS03685 - serine/threonine protein phosphatase, at 877,230 to 877,961
_RS03685
CA265_RS03690: CA265_RS03690 - nicotinate phosphoribosyltransferase, at 877,986 to 879,443
_RS03690
CA265_RS03695: CA265_RS03695 - hypothetical protein, at 879,502 to 879,906
_RS03695
CA265_RS03700: CA265_RS03700 - hypothetical protein, at 879,947 to 880,477
_RS03700
Position (kb) 877
878
879
880 Strain fitness (log2 ratio) -2
-1
0
1
2 at 877.015 kb on - strand, within CA265_RS03680 at 877.086 kb on + strand, within CA265_RS03680 at 877.099 kb on - strand, within CA265_RS03680 at 877.116 kb on + strand, within CA265_RS03680 at 877.118 kb on + strand, within CA265_RS03680 at 877.216 kb on - strand at 877.218 kb on - strand at 877.243 kb on + strand at 877.247 kb on - strand at 877.250 kb on - strand at 877.256 kb on + strand at 877.257 kb on - strand at 877.257 kb on - strand at 877.306 kb on + strand, within CA265_RS03685 at 877.341 kb on + strand, within CA265_RS03685 at 877.373 kb on - strand, within CA265_RS03685 at 877.467 kb on - strand, within CA265_RS03685 at 877.471 kb on + strand, within CA265_RS03685 at 877.472 kb on - strand, within CA265_RS03685 at 877.472 kb on - strand, within CA265_RS03685 at 877.472 kb on - strand, within CA265_RS03685 at 877.508 kb on + strand, within CA265_RS03685 at 877.518 kb on + strand, within CA265_RS03685 at 877.656 kb on + strand, within CA265_RS03685 at 877.657 kb on - strand, within CA265_RS03685 at 877.669 kb on - strand, within CA265_RS03685 at 877.736 kb on - strand, within CA265_RS03685 at 877.801 kb on - strand, within CA265_RS03685 at 877.801 kb on - strand, within CA265_RS03685 at 877.803 kb on - strand, within CA265_RS03685 at 877.803 kb on - strand, within CA265_RS03685 at 877.803 kb on - strand, within CA265_RS03685 at 877.829 kb on + strand, within CA265_RS03685 at 877.830 kb on - strand, within CA265_RS03685 at 877.873 kb on + strand, within CA265_RS03685 at 877.922 kb on - strand at 877.974 kb on + strand at 877.980 kb on - strand at 877.999 kb on + strand at 877.999 kb on + strand at 878.020 kb on + strand at 878.021 kb on - strand at 878.048 kb on + strand at 878.101 kb on + strand at 878.150 kb on + strand, within CA265_RS03690 at 878.151 kb on - strand, within CA265_RS03690 at 878.162 kb on + strand, within CA265_RS03690 at 878.294 kb on + strand, within CA265_RS03690 at 878.295 kb on - strand, within CA265_RS03690 at 878.304 kb on - strand, within CA265_RS03690 at 878.351 kb on + strand, within CA265_RS03690 at 878.358 kb on + strand, within CA265_RS03690 at 878.358 kb on + strand, within CA265_RS03690 at 878.358 kb on + strand, within CA265_RS03690 at 878.359 kb on - strand, within CA265_RS03690 at 878.359 kb on - strand, within CA265_RS03690 at 878.391 kb on - strand, within CA265_RS03690 at 878.407 kb on + strand, within CA265_RS03690 at 878.408 kb on - strand, within CA265_RS03690 at 878.481 kb on + strand, within CA265_RS03690 at 878.481 kb on + strand, within CA265_RS03690 at 878.482 kb on - strand, within CA265_RS03690 at 878.482 kb on - strand, within CA265_RS03690 at 878.541 kb on - strand, within CA265_RS03690 at 878.646 kb on + strand, within CA265_RS03690 at 878.647 kb on - strand, within CA265_RS03690 at 878.650 kb on - strand, within CA265_RS03690 at 878.789 kb on - strand, within CA265_RS03690 at 878.824 kb on + strand, within CA265_RS03690 at 878.825 kb on - strand, within CA265_RS03690 at 878.858 kb on + strand, within CA265_RS03690 at 878.859 kb on - strand, within CA265_RS03690 at 878.859 kb on - strand, within CA265_RS03690 at 878.966 kb on - strand, within CA265_RS03690 at 878.966 kb on - strand, within CA265_RS03690 at 878.987 kb on - strand, within CA265_RS03690 at 879.002 kb on + strand, within CA265_RS03690 at 879.003 kb on - strand, within CA265_RS03690 at 879.056 kb on + strand, within CA265_RS03690 at 879.057 kb on - strand, within CA265_RS03690 at 879.060 kb on - strand, within CA265_RS03690 at 879.060 kb on - strand, within CA265_RS03690 at 879.119 kb on + strand, within CA265_RS03690 at 879.120 kb on - strand, within CA265_RS03690 at 879.150 kb on - strand, within CA265_RS03690 at 879.164 kb on + strand, within CA265_RS03690 at 879.201 kb on + strand, within CA265_RS03690 at 879.201 kb on + strand, within CA265_RS03690 at 879.202 kb on - strand, within CA265_RS03690 at 879.202 kb on - strand, within CA265_RS03690 at 879.202 kb on - strand, within CA265_RS03690 at 879.211 kb on + strand, within CA265_RS03690 at 879.315 kb on + strand at 879.391 kb on + strand at 879.391 kb on + strand at 879.403 kb on - strand at 879.485 kb on + strand at 879.498 kb on + strand at 879.543 kb on - strand, within CA265_RS03695 at 879.562 kb on - strand, within CA265_RS03695 at 879.563 kb on + strand, within CA265_RS03695 at 879.585 kb on + strand, within CA265_RS03695 at 879.586 kb on - strand, within CA265_RS03695 at 879.586 kb on - strand, within CA265_RS03695 at 879.618 kb on + strand, within CA265_RS03695 at 879.697 kb on + strand, within CA265_RS03695 at 879.697 kb on + strand, within CA265_RS03695 at 879.697 kb on + strand, within CA265_RS03695 at 879.698 kb on - strand, within CA265_RS03695 at 879.720 kb on + strand, within CA265_RS03695 at 879.835 kb on - strand, within CA265_RS03695 at 879.855 kb on + strand, within CA265_RS03695 at 879.855 kb on + strand, within CA265_RS03695 at 879.856 kb on - strand, within CA265_RS03695 at 879.978 kb on + strand at 879.994 kb on + strand at 880.034 kb on + strand, within CA265_RS03700 at 880.035 kb on - strand, within CA265_RS03700 at 880.036 kb on + strand, within CA265_RS03700 at 880.036 kb on + strand, within CA265_RS03700 at 880.038 kb on + strand, within CA265_RS03700 at 880.039 kb on - strand, within CA265_RS03700 at 880.039 kb on - strand, within CA265_RS03700 at 880.039 kb on - strand, within CA265_RS03700 at 880.093 kb on + strand, within CA265_RS03700 at 880.127 kb on + strand, within CA265_RS03700 at 880.142 kb on - strand, within CA265_RS03700 at 880.183 kb on + strand, within CA265_RS03700 at 880.188 kb on + strand, within CA265_RS03700 at 880.188 kb on + strand, within CA265_RS03700 at 880.195 kb on + strand, within CA265_RS03700 at 880.196 kb on - strand, within CA265_RS03700 at 880.196 kb on - strand, within CA265_RS03700 at 880.196 kb on - strand, within CA265_RS03700 at 880.206 kb on - strand, within CA265_RS03700 at 880.208 kb on - strand, within CA265_RS03700 at 880.227 kb on + strand, within CA265_RS03700 at 880.228 kb on - strand, within CA265_RS03700 at 880.298 kb on + strand, within CA265_RS03700 at 880.418 kb on + strand, within CA265_RS03700 at 880.419 kb on - strand, within CA265_RS03700 at 880.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 877,015 - CA265_RS03680 0.61 -0.5 877,086 + CA265_RS03680 0.74 -0.2 877,099 - CA265_RS03680 0.76 -0.3 877,116 + CA265_RS03680 0.79 +1.2 877,118 + CA265_RS03680 0.80 +0.8 877,216 - -0.4 877,218 - -0.7 877,243 + +0.3 877,247 - -2.2 877,250 - -0.7 877,256 + +0.2 877,257 - +1.3 877,257 - +0.2 877,306 + CA265_RS03685 0.10 +0.6 877,341 + CA265_RS03685 0.15 -0.5 877,373 - CA265_RS03685 0.20 +0.2 877,467 - CA265_RS03685 0.32 +1.9 877,471 + CA265_RS03685 0.33 -0.7 877,472 - CA265_RS03685 0.33 -0.4 877,472 - CA265_RS03685 0.33 -1.1 877,472 - CA265_RS03685 0.33 -1.1 877,508 + CA265_RS03685 0.38 +0.5 877,518 + CA265_RS03685 0.39 -0.1 877,656 + CA265_RS03685 0.58 +0.7 877,657 - CA265_RS03685 0.58 -0.4 877,669 - CA265_RS03685 0.60 -0.0 877,736 - CA265_RS03685 0.69 +0.4 877,801 - CA265_RS03685 0.78 -0.9 877,801 - CA265_RS03685 0.78 +0.3 877,803 - CA265_RS03685 0.78 -0.8 877,803 - CA265_RS03685 0.78 +0.2 877,803 - CA265_RS03685 0.78 +0.2 877,829 + CA265_RS03685 0.82 -0.3 877,830 - CA265_RS03685 0.82 -0.2 877,873 + CA265_RS03685 0.88 -0.2 877,922 - -0.8 877,974 + +0.9 877,980 - +0.1 877,999 + +1.1 877,999 + +0.4 878,020 + -0.8 878,021 - -2.1 878,048 + +0.7 878,101 + -0.1 878,150 + CA265_RS03690 0.11 -1.2 878,151 - CA265_RS03690 0.11 -0.9 878,162 + CA265_RS03690 0.12 +0.2 878,294 + CA265_RS03690 0.21 -0.1 878,295 - CA265_RS03690 0.21 -0.3 878,304 - CA265_RS03690 0.22 -0.5 878,351 + CA265_RS03690 0.25 -1.3 878,358 + CA265_RS03690 0.26 -0.4 878,358 + CA265_RS03690 0.26 -0.7 878,358 + CA265_RS03690 0.26 -1.0 878,359 - CA265_RS03690 0.26 -1.0 878,359 - CA265_RS03690 0.26 -0.0 878,391 - CA265_RS03690 0.28 -0.4 878,407 + CA265_RS03690 0.29 -0.9 878,408 - CA265_RS03690 0.29 -0.3 878,481 + CA265_RS03690 0.34 +0.6 878,481 + CA265_RS03690 0.34 +0.6 878,482 - CA265_RS03690 0.34 -0.6 878,482 - CA265_RS03690 0.34 +0.5 878,541 - CA265_RS03690 0.38 +0.8 878,646 + CA265_RS03690 0.45 +0.2 878,647 - CA265_RS03690 0.45 +0.3 878,650 - CA265_RS03690 0.46 +2.0 878,789 - CA265_RS03690 0.55 +0.5 878,824 + CA265_RS03690 0.57 +0.0 878,825 - CA265_RS03690 0.58 -0.0 878,858 + CA265_RS03690 0.60 +0.7 878,859 - CA265_RS03690 0.60 -0.1 878,859 - CA265_RS03690 0.60 -0.4 878,966 - CA265_RS03690 0.67 +0.5 878,966 - CA265_RS03690 0.67 -0.1 878,987 - CA265_RS03690 0.69 -0.5 879,002 + CA265_RS03690 0.70 +1.5 879,003 - CA265_RS03690 0.70 -0.1 879,056 + CA265_RS03690 0.73 -1.0 879,057 - CA265_RS03690 0.73 +0.5 879,060 - CA265_RS03690 0.74 +0.1 879,060 - CA265_RS03690 0.74 +0.1 879,119 + CA265_RS03690 0.78 -1.3 879,120 - CA265_RS03690 0.78 -0.1 879,150 - CA265_RS03690 0.80 -0.1 879,164 + CA265_RS03690 0.81 +0.3 879,201 + CA265_RS03690 0.83 -1.1 879,201 + CA265_RS03690 0.83 +0.2 879,202 - CA265_RS03690 0.83 -0.9 879,202 - CA265_RS03690 0.83 -0.1 879,202 - CA265_RS03690 0.83 -0.4 879,211 + CA265_RS03690 0.84 +0.7 879,315 + +2.0 879,391 + -1.0 879,391 + +1.2 879,403 - -0.3 879,485 + -0.9 879,498 + -0.7 879,543 - CA265_RS03695 0.10 +0.6 879,562 - CA265_RS03695 0.15 +0.5 879,563 + CA265_RS03695 0.15 -0.3 879,585 + CA265_RS03695 0.20 -0.8 879,586 - CA265_RS03695 0.21 -1.1 879,586 - CA265_RS03695 0.21 -0.3 879,618 + CA265_RS03695 0.29 +2.5 879,697 + CA265_RS03695 0.48 +1.0 879,697 + CA265_RS03695 0.48 -0.1 879,697 + CA265_RS03695 0.48 +0.2 879,698 - CA265_RS03695 0.48 -0.0 879,720 + CA265_RS03695 0.54 +1.9 879,835 - CA265_RS03695 0.82 +0.1 879,855 + CA265_RS03695 0.87 +1.2 879,855 + CA265_RS03695 0.87 -0.0 879,856 - CA265_RS03695 0.87 +0.2 879,978 + -0.4 879,994 + -1.6 880,034 + CA265_RS03700 0.16 -1.1 880,035 - CA265_RS03700 0.17 -2.5 880,036 + CA265_RS03700 0.17 -0.1 880,036 + CA265_RS03700 0.17 -0.7 880,038 + CA265_RS03700 0.17 -0.2 880,039 - CA265_RS03700 0.17 -0.2 880,039 - CA265_RS03700 0.17 -0.8 880,039 - CA265_RS03700 0.17 -0.6 880,093 + CA265_RS03700 0.27 -1.1 880,127 + CA265_RS03700 0.34 -0.2 880,142 - CA265_RS03700 0.37 +0.1 880,183 + CA265_RS03700 0.44 -0.7 880,188 + CA265_RS03700 0.45 -1.1 880,188 + CA265_RS03700 0.45 -0.1 880,195 + CA265_RS03700 0.47 +0.0 880,196 - CA265_RS03700 0.47 +0.0 880,196 - CA265_RS03700 0.47 +1.1 880,196 - CA265_RS03700 0.47 -0.9 880,206 - CA265_RS03700 0.49 -0.4 880,208 - CA265_RS03700 0.49 -0.1 880,227 + CA265_RS03700 0.53 +0.0 880,228 - CA265_RS03700 0.53 -0.2 880,298 + CA265_RS03700 0.66 -0.0 880,418 + CA265_RS03700 0.89 -0.5 880,419 - CA265_RS03700 0.89 -0.6 880,441 - -0.3
Or see this region's nucleotide sequence