Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00775 and CA265_RS00780 are separated by 60 nucleotides CA265_RS00780 and CA265_RS00785 are separated by 295 nucleotides CA265_RS00785 and CA265_RS00790 are separated by 310 nucleotides
CA265_RS00775: CA265_RS00775 - carbonic anhydrase, at 186,125 to 186,751
_RS00775
CA265_RS00780: CA265_RS00780 - hypothetical protein, at 186,812 to 187,321
_RS00780
CA265_RS00785: CA265_RS00785 - hypothetical protein, at 187,617 to 188,228
_RS00785
CA265_RS00790: CA265_RS00790 - Crp/Fnr family transcriptional regulator, at 188,539 to 189,180
_RS00790
Position (kb) 187
188
189 Strain fitness (log2 ratio) -2
-1
0
1
2 at 186.623 kb on - strand, within CA265_RS00775 at 186.639 kb on + strand, within CA265_RS00775 at 186.691 kb on + strand at 186.743 kb on + strand at 186.743 kb on + strand at 186.783 kb on + strand at 186.821 kb on + strand at 186.823 kb on + strand at 186.891 kb on + strand, within CA265_RS00780 at 187.093 kb on - strand, within CA265_RS00780 at 187.117 kb on + strand, within CA265_RS00780 at 187.118 kb on - strand, within CA265_RS00780 at 187.126 kb on + strand, within CA265_RS00780 at 187.127 kb on - strand, within CA265_RS00780 at 187.131 kb on + strand, within CA265_RS00780 at 187.132 kb on - strand, within CA265_RS00780 at 187.308 kb on - strand at 187.340 kb on - strand at 187.340 kb on - strand at 187.360 kb on + strand at 187.360 kb on + strand at 187.360 kb on + strand at 187.424 kb on + strand at 187.425 kb on - strand at 187.425 kb on - strand at 187.430 kb on - strand at 187.453 kb on - strand at 187.576 kb on + strand at 187.577 kb on - strand at 187.673 kb on + strand at 187.673 kb on + strand at 187.676 kb on - strand at 187.717 kb on + strand, within CA265_RS00785 at 187.718 kb on - strand, within CA265_RS00785 at 187.718 kb on - strand, within CA265_RS00785 at 187.718 kb on - strand, within CA265_RS00785 at 187.759 kb on - strand, within CA265_RS00785 at 187.766 kb on + strand, within CA265_RS00785 at 187.855 kb on - strand, within CA265_RS00785 at 187.987 kb on + strand, within CA265_RS00785 at 188.129 kb on + strand, within CA265_RS00785 at 188.206 kb on + strand at 188.232 kb on + strand at 188.256 kb on + strand at 188.256 kb on + strand at 188.257 kb on - strand at 188.295 kb on - strand at 188.299 kb on - strand at 188.323 kb on - strand at 188.392 kb on - strand at 188.393 kb on + strand at 188.394 kb on - strand at 188.394 kb on - strand at 188.395 kb on + strand at 188.396 kb on - strand at 188.519 kb on + strand at 188.520 kb on - strand at 188.530 kb on + strand at 188.585 kb on - strand at 188.585 kb on - strand at 188.592 kb on + strand at 188.592 kb on + strand at 188.620 kb on - strand, within CA265_RS00790 at 188.750 kb on - strand, within CA265_RS00790 at 188.750 kb on - strand, within CA265_RS00790 at 188.754 kb on + strand, within CA265_RS00790 at 188.756 kb on + strand, within CA265_RS00790 at 188.756 kb on + strand, within CA265_RS00790 at 188.756 kb on + strand, within CA265_RS00790 at 188.757 kb on - strand, within CA265_RS00790 at 188.758 kb on + strand, within CA265_RS00790 at 188.759 kb on - strand, within CA265_RS00790 at 188.791 kb on + strand, within CA265_RS00790 at 188.845 kb on - strand, within CA265_RS00790 at 188.877 kb on + strand, within CA265_RS00790 at 188.899 kb on + strand, within CA265_RS00790 at 188.913 kb on + strand, within CA265_RS00790 at 188.914 kb on - strand, within CA265_RS00790 at 188.914 kb on - strand, within CA265_RS00790 at 188.936 kb on - strand, within CA265_RS00790 at 188.967 kb on + strand, within CA265_RS00790 at 188.968 kb on - strand, within CA265_RS00790 at 189.180 kb on + strand at 189.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 186,623 - CA265_RS00775 0.79 +1.2 186,639 + CA265_RS00775 0.82 +0.3 186,691 + -0.5 186,743 + -0.3 186,743 + +0.3 186,783 + +1.5 186,821 + -0.1 186,823 + +0.5 186,891 + CA265_RS00780 0.15 -0.2 187,093 - CA265_RS00780 0.55 +0.5 187,117 + CA265_RS00780 0.60 +0.0 187,118 - CA265_RS00780 0.60 +0.2 187,126 + CA265_RS00780 0.62 +0.3 187,127 - CA265_RS00780 0.62 +0.8 187,131 + CA265_RS00780 0.63 +0.9 187,132 - CA265_RS00780 0.63 +0.5 187,308 - +0.7 187,340 - +1.5 187,340 - -0.2 187,360 + +0.5 187,360 + -2.3 187,360 + +1.8 187,424 + +1.1 187,425 - -0.2 187,425 - +0.2 187,430 - -1.3 187,453 - +0.5 187,576 + +0.3 187,577 - -0.4 187,673 + +1.0 187,673 + +0.9 187,676 - +0.2 187,717 + CA265_RS00785 0.16 +2.5 187,718 - CA265_RS00785 0.17 -1.5 187,718 - CA265_RS00785 0.17 +0.7 187,718 - CA265_RS00785 0.17 +1.2 187,759 - CA265_RS00785 0.23 +0.0 187,766 + CA265_RS00785 0.24 +1.3 187,855 - CA265_RS00785 0.39 +1.4 187,987 + CA265_RS00785 0.60 +1.4 188,129 + CA265_RS00785 0.84 +1.3 188,206 + +1.5 188,232 + +0.9 188,256 + +1.0 188,256 + +2.6 188,257 - +0.3 188,295 - +0.8 188,299 - +0.8 188,323 - -0.5 188,392 - -1.0 188,393 + +1.5 188,394 - +0.2 188,394 - +0.5 188,395 + +1.0 188,396 - +0.5 188,519 + +0.1 188,520 - -0.6 188,530 + +0.2 188,585 - -0.1 188,585 - -0.5 188,592 + +0.7 188,592 + -0.8 188,620 - CA265_RS00790 0.13 -0.2 188,750 - CA265_RS00790 0.33 +1.9 188,750 - CA265_RS00790 0.33 +0.2 188,754 + CA265_RS00790 0.33 -0.2 188,756 + CA265_RS00790 0.34 -1.4 188,756 + CA265_RS00790 0.34 +1.1 188,756 + CA265_RS00790 0.34 -0.1 188,757 - CA265_RS00790 0.34 +0.2 188,758 + CA265_RS00790 0.34 +0.4 188,759 - CA265_RS00790 0.34 +0.7 188,791 + CA265_RS00790 0.39 -0.3 188,845 - CA265_RS00790 0.48 -0.4 188,877 + CA265_RS00790 0.53 -0.4 188,899 + CA265_RS00790 0.56 +0.9 188,913 + CA265_RS00790 0.58 -0.2 188,914 - CA265_RS00790 0.58 -1.2 188,914 - CA265_RS00790 0.58 -0.4 188,936 - CA265_RS00790 0.62 -0.4 188,967 + CA265_RS00790 0.67 -0.7 188,968 - CA265_RS00790 0.67 +0.0 189,180 + +1.0 189,181 - +0.1
Or see this region's nucleotide sequence