Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_14140 and AO353_14145 overlap by 8 nucleotides AO353_14145 and AO353_14150 overlap by 8 nucleotides AO353_14150 and AO353_14155 are separated by 133 nucleotides
AO353_14140: AO353_14140 - isoleucine--tRNA ligase, at 3,140,727 to 3,143,558
_14140
AO353_14145: AO353_14145 - signal peptidase II, at 3,143,551 to 3,144,063
_14145
AO353_14150: AO353_14150 - peptidylprolyl isomerase, at 3,144,056 to 3,144,508
_14150
AO353_14155: AO353_14155 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 3,144,642 to 3,145,589
_14155
Position (kb)
3144
3145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3144.021 kb on + strand at 3144.021 kb on + strand at 3144.021 kb on + strand at 3144.021 kb on + strand at 3144.069 kb on + strand at 3144.154 kb on + strand, within AO353_14150 at 3144.253 kb on + strand, within AO353_14150 at 3144.253 kb on + strand, within AO353_14150 at 3144.480 kb on + strand at 3144.480 kb on + strand at 3144.506 kb on + strand at 3144.506 kb on + strand at 3144.506 kb on + strand at 3144.513 kb on + strand at 3144.610 kb on + strand at 3144.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,144,021 + -1.8 3,144,021 + +1.8 3,144,021 + -0.8 3,144,021 + -0.8 3,144,069 + -1.1 3,144,154 + AO353_14150 0.22 -1.3 3,144,253 + AO353_14150 0.43 -1.8 3,144,253 + AO353_14150 0.43 -2.1 3,144,480 + +1.8 3,144,480 + -0.2 3,144,506 + -1.8 3,144,506 + -1.8 3,144,506 + +1.1 3,144,513 + -1.8 3,144,610 + -2.8 3,144,610 + +0.2
Or see this region's nucleotide sequence