Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2653 and DvMF_2654 are separated by 171 nucleotides DvMF_2654 and DvMF_2655 are separated by 213 nucleotides DvMF_2655 and DvMF_2656 are separated by 274 nucleotides DvMF_2656 and DvMF_2657 are separated by 131 nucleotides
DvMF_2653: DvMF_2653 - Carboxymuconolactone decarboxylase (RefSeq), at 3,353,841 to 3,354,257
_2653
DvMF_2654: DvMF_2654 - acetyltransferase (RefSeq), at 3,354,429 to 3,354,938
_2654
DvMF_2655: DvMF_2655 - hypothetical protein (RefSeq), at 3,355,152 to 3,355,688
_2655
DvMF_2656: DvMF_2656 - Glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 3,355,963 to 3,356,385
_2656
DvMF_2657: DvMF_2657 - Serine O-acetyltransferase (RefSeq), at 3,356,517 to 3,357,470
_2657
Position (kb)
3355
3356 Strain fitness (log2 ratio)
-1
0
1 at 3354.183 kb on - strand, within DvMF_2653 at 3354.384 kb on - strand at 3355.080 kb on + strand at 3355.296 kb on - strand, within DvMF_2655 at 3355.396 kb on + strand, within DvMF_2655 at 3355.445 kb on + strand, within DvMF_2655 at 3355.453 kb on - strand, within DvMF_2655 at 3355.463 kb on - strand, within DvMF_2655 at 3355.464 kb on - strand, within DvMF_2655 at 3355.484 kb on + strand, within DvMF_2655 at 3355.692 kb on + strand at 3356.140 kb on + strand, within DvMF_2656 at 3356.166 kb on + strand, within DvMF_2656 at 3356.357 kb on - strand at 3356.434 kb on + strand at 3356.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 3,354,183 - DvMF_2653 0.82 -1.0 3,354,384 - -1.4 3,355,080 + -1.5 3,355,296 - DvMF_2655 0.27 -0.6 3,355,396 + DvMF_2655 0.45 +0.4 3,355,445 + DvMF_2655 0.55 -0.7 3,355,453 - DvMF_2655 0.56 +0.4 3,355,463 - DvMF_2655 0.58 -1.2 3,355,464 - DvMF_2655 0.58 +0.1 3,355,484 + DvMF_2655 0.62 -0.8 3,355,692 + -0.5 3,356,140 + DvMF_2656 0.42 -0.2 3,356,166 + DvMF_2656 0.48 -1.2 3,356,357 - -0.6 3,356,434 + -0.4 3,356,553 - -0.8
Or see this region's nucleotide sequence