Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2312 and DvMF_2313 are separated by 366 nucleotides DvMF_2313 and DvMF_2314 are separated by 50 nucleotides DvMF_2314 and DvMF_2315 overlap by 4 nucleotides
DvMF_2312: DvMF_2312 - metallophosphoesterase (RefSeq), at 2,909,594 to 2,911,135
_2312
DvMF_2313: DvMF_2313 - DNA-directed DNA polymerase (RefSeq), at 2,911,502 to 2,912,947
_2313
DvMF_2314: DvMF_2314 - aminotransferase class IV (RefSeq), at 2,912,998 to 2,913,771
_2314
DvMF_2315: DvMF_2315 - Chorismate binding-like protein (RefSeq), at 2,913,768 to 2,915,411
_2315
Position (kb)
2911
2912
2913 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2910.508 kb on - strand, within DvMF_2312 at 2910.575 kb on + strand, within DvMF_2312 at 2910.630 kb on + strand, within DvMF_2312 at 2910.638 kb on - strand, within DvMF_2312 at 2910.910 kb on + strand, within DvMF_2312 at 2910.924 kb on - strand, within DvMF_2312 at 2910.945 kb on - strand, within DvMF_2312 at 2911.344 kb on + strand at 2911.523 kb on - strand at 2911.680 kb on + strand, within DvMF_2313 at 2911.859 kb on - strand, within DvMF_2313 at 2911.869 kb on - strand, within DvMF_2313 at 2911.996 kb on + strand, within DvMF_2313 at 2912.046 kb on + strand, within DvMF_2313 at 2912.111 kb on + strand, within DvMF_2313 at 2912.179 kb on + strand, within DvMF_2313 at 2912.240 kb on + strand, within DvMF_2313 at 2912.394 kb on + strand, within DvMF_2313 at 2912.400 kb on + strand, within DvMF_2313 at 2912.510 kb on - strand, within DvMF_2313 at 2912.980 kb on + strand at 2913.192 kb on - strand, within DvMF_2314 at 2913.194 kb on + strand, within DvMF_2314 at 2913.796 kb on + strand at 2913.796 kb on + strand at 2913.845 kb on - strand at 2913.899 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 2,910,508 - DvMF_2312 0.59 -1.2 2,910,575 + DvMF_2312 0.64 -0.9 2,910,630 + DvMF_2312 0.67 -0.7 2,910,638 - DvMF_2312 0.68 -1.2 2,910,910 + DvMF_2312 0.85 -0.8 2,910,924 - DvMF_2312 0.86 -1.1 2,910,945 - DvMF_2312 0.88 -1.2 2,911,344 + -0.4 2,911,523 - -0.7 2,911,680 + DvMF_2313 0.12 -0.7 2,911,859 - DvMF_2313 0.25 -1.0 2,911,869 - DvMF_2313 0.25 -1.1 2,911,996 + DvMF_2313 0.34 -3.2 2,912,046 + DvMF_2313 0.38 -1.9 2,912,111 + DvMF_2313 0.42 -0.5 2,912,179 + DvMF_2313 0.47 -0.7 2,912,240 + DvMF_2313 0.51 -1.6 2,912,394 + DvMF_2313 0.62 -1.7 2,912,400 + DvMF_2313 0.62 -1.2 2,912,510 - DvMF_2313 0.70 -3.4 2,912,980 + -2.0 2,913,192 - DvMF_2314 0.25 +0.1 2,913,194 + DvMF_2314 0.25 -0.8 2,913,796 + -0.6 2,913,796 + -0.1 2,913,845 - -0.9 2,913,899 - +0.1
Or see this region's nucleotide sequence