Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2293 and DvMF_2294 are separated by 649 nucleotides DvMF_2294 and DvMF_2295 are separated by 6 nucleotides DvMF_2295 and DvMF_2296 are separated by 256 nucleotides DvMF_2296 and DvMF_2297 are separated by 39 nucleotides
DvMF_2293: DvMF_2293 - PP-loop domain protein (RefSeq), at 2,881,458 to 2,882,204
_2293
DvMF_2294: DvMF_2294 - DNA-directed RNA polymerase, omega subunit (RefSeq), at 2,882,854 to 2,883,081
_2294
DvMF_2295: DvMF_2295 - chaperone protein DnaJ (RefSeq), at 2,883,088 to 2,884,212
_2295
DvMF_2296: DvMF_2296 - hypothetical protein (RefSeq), at 2,884,469 to 2,885,122
_2296
DvMF_2297: DvMF_2297 - hypothetical protein (RefSeq), at 2,885,162 to 2,885,677
_2297
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2882.171 kb on + strand at 2882.179 kb on + strand at 2882.464 kb on - strand at 2882.768 kb on + strand at 2883.112 kb on - strand at 2883.647 kb on + strand, within DvMF_2295 at 2883.855 kb on + strand, within DvMF_2295 at 2884.034 kb on + strand, within DvMF_2295 at 2884.088 kb on + strand, within DvMF_2295 at 2884.554 kb on + strand, within DvMF_2296 at 2884.640 kb on - strand, within DvMF_2296 at 2884.668 kb on - strand, within DvMF_2296 at 2884.702 kb on + strand, within DvMF_2296 at 2884.735 kb on + strand, within DvMF_2296 at 2884.951 kb on - strand, within DvMF_2296 at 2885.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 2,882,171 + +0.4 2,882,179 + +1.7 2,882,464 - -0.7 2,882,768 + +0.6 2,883,112 - +1.7 2,883,647 + DvMF_2295 0.50 +2.4 2,883,855 + DvMF_2295 0.68 +2.1 2,884,034 + DvMF_2295 0.84 +2.1 2,884,088 + DvMF_2295 0.89 +1.5 2,884,554 + DvMF_2296 0.13 -1.4 2,884,640 - DvMF_2296 0.26 -3.1 2,884,668 - DvMF_2296 0.30 -0.1 2,884,702 + DvMF_2296 0.36 +0.8 2,884,735 + DvMF_2296 0.41 -0.2 2,884,951 - DvMF_2296 0.74 -1.0 2,885,067 + -0.6
Or see this region's nucleotide sequence