Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and DvMF_2216 are separated by 13 nucleotides DvMF_2216 and DvMF_2217 are separated by 179 nucleotides DvMF_2217 and DvMF_2218 are separated by 655 nucleotides
DvMF_2215: fliP - flagellar biosynthesis protein FliP (RefSeq), at 2,767,902 to 2,768,783
fliP
DvMF_2216: DvMF_2216 - flagellar biosynthetic protein FliQ (RefSeq), at 2,768,797 to 2,769,066
_2216
DvMF_2217: DvMF_2217 - tRNA/rRNA methyltransferase (SpoU) (RefSeq), at 2,769,246 to 2,770,280
_2217
DvMF_2218: DvMF_2218 - Lytic transglycosylase catalytic (RefSeq), at 2,770,936 to 2,772,792
_2218
Position (kb)
2769
2770
2771 Strain fitness (log2 ratio)
-1
0
1 at 2768.299 kb on - strand, within fliP at 2768.315 kb on - strand, within fliP at 2768.466 kb on - strand, within fliP at 2768.575 kb on + strand, within fliP at 2768.892 kb on - strand, within DvMF_2216 at 2769.297 kb on - strand at 2769.309 kb on - strand at 2769.353 kb on - strand, within DvMF_2217 at 2769.617 kb on - strand, within DvMF_2217 at 2770.026 kb on - strand, within DvMF_2217 at 2770.026 kb on - strand, within DvMF_2217 at 2770.223 kb on + strand at 2770.375 kb on - strand at 2770.797 kb on - strand at 2770.958 kb on + strand at 2770.958 kb on + strand at 2771.081 kb on - strand at 2771.116 kb on - strand at 2771.144 kb on - strand, within DvMF_2218 at 2771.178 kb on + strand, within DvMF_2218
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 2,768,299 - fliP DvMF_2215 0.45 +0.3 2,768,315 - fliP DvMF_2215 0.47 -0.0 2,768,466 - fliP DvMF_2215 0.64 +0.2 2,768,575 + fliP DvMF_2215 0.76 +0.9 2,768,892 - DvMF_2216 0.35 -0.5 2,769,297 - -0.5 2,769,309 - -0.5 2,769,353 - DvMF_2217 0.10 +0.1 2,769,617 - DvMF_2217 0.36 +0.2 2,770,026 - DvMF_2217 0.75 -0.5 2,770,026 - DvMF_2217 0.75 -0.3 2,770,223 + -0.2 2,770,375 - +0.1 2,770,797 - +0.2 2,770,958 + +0.6 2,770,958 + +0.4 2,771,081 - +0.7 2,771,116 - -0.4 2,771,144 - DvMF_2218 0.11 -1.1 2,771,178 + DvMF_2218 0.13 +0.3
Or see this region's nucleotide sequence