Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2143 and DvMF_2144 are separated by 94 nucleotides DvMF_2144 and DvMF_2145 overlap by 4 nucleotides DvMF_2145 and DvMF_2146 are separated by 325 nucleotides
DvMF_2143: DvMF_2143 - dihydroorotate dehydrogenase family protein (RefSeq), at 2,669,027 to 2,669,953
_2143
DvMF_2144: DvMF_2144 - GCN5-related N-acetyltransferase (RefSeq), at 2,670,048 to 2,670,587
_2144
DvMF_2145: DvMF_2145 - Protein of unknown function DUF1778 (RefSeq), at 2,670,584 to 2,670,856
_2145
DvMF_2146: DvMF_2146 - delta-1-pyrroline-5-carboxylate dehydrogenase (RefSeq), at 2,671,182 to 2,674,223
_2146
Position (kb)
2670
2671 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2669.167 kb on + strand, within DvMF_2143 at 2669.197 kb on + strand, within DvMF_2143 at 2669.197 kb on + strand, within DvMF_2143 at 2669.485 kb on + strand, within DvMF_2143 at 2669.657 kb on - strand, within DvMF_2143 at 2669.665 kb on + strand, within DvMF_2143 at 2669.929 kb on + strand at 2670.045 kb on + strand at 2670.045 kb on + strand at 2670.053 kb on - strand at 2670.053 kb on - strand at 2670.281 kb on + strand, within DvMF_2144 at 2670.416 kb on - strand, within DvMF_2144 at 2670.755 kb on + strand, within DvMF_2145 at 2671.187 kb on - strand at 2671.370 kb on + strand at 2671.456 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 2,669,167 + DvMF_2143 0.15 +0.8 2,669,197 + DvMF_2143 0.18 +0.9 2,669,197 + DvMF_2143 0.18 -0.0 2,669,485 + DvMF_2143 0.49 +0.2 2,669,657 - DvMF_2143 0.68 -1.1 2,669,665 + DvMF_2143 0.69 +1.8 2,669,929 + -0.1 2,670,045 + +1.6 2,670,045 + -0.4 2,670,053 - -0.3 2,670,053 - -0.8 2,670,281 + DvMF_2144 0.43 -0.3 2,670,416 - DvMF_2144 0.68 +1.7 2,670,755 + DvMF_2145 0.63 -4.0 2,671,187 - -0.5 2,671,370 + -3.2 2,671,456 - -1.2
Or see this region's nucleotide sequence