Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2019 and DvMF_2020 overlap by 4 nucleotides DvMF_2020 and DvMF_2021 are separated by 128 nucleotides DvMF_2021 and DvMF_2022 are separated by 355 nucleotides
DvMF_2019: DvMF_2019 - dihydrouridine synthase DuS (RefSeq), at 2,500,863 to 2,501,918
_2019
DvMF_2020: DvMF_2020 - hydroxymethylbutenyl pyrophosphate reductase (RefSeq), at 2,501,915 to 2,502,760
_2020
DvMF_2021: DvMF_2021 - response regulator receiver modulated CheW protein (RefSeq), at 2,502,889 to 2,503,839
_2021
DvMF_2022: DvMF_2022 - transcriptional regulator, GntR family with aminotransferase domain (RefSeq), at 2,504,195 to 2,505,976
_2022
Position (kb)
2502
2503
2504 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2502.996 kb on + strand, within DvMF_2021 at 2503.006 kb on - strand, within DvMF_2021 at 2503.362 kb on + strand, within DvMF_2021 at 2503.540 kb on + strand, within DvMF_2021 at 2503.649 kb on + strand, within DvMF_2021 at 2503.657 kb on - strand, within DvMF_2021 at 2503.668 kb on + strand, within DvMF_2021 at 2503.668 kb on + strand, within DvMF_2021 at 2503.668 kb on + strand, within DvMF_2021 at 2504.084 kb on - strand at 2504.114 kb on - strand at 2504.134 kb on - strand at 2504.335 kb on - strand at 2504.351 kb on - strand at 2504.359 kb on - strand at 2504.830 kb on + strand, within DvMF_2022
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 2,502,996 + DvMF_2021 0.11 +0.2 2,503,006 - DvMF_2021 0.12 +0.0 2,503,362 + DvMF_2021 0.50 +0.5 2,503,540 + DvMF_2021 0.68 +0.6 2,503,649 + DvMF_2021 0.80 -0.2 2,503,657 - DvMF_2021 0.81 -0.7 2,503,668 + DvMF_2021 0.82 +0.8 2,503,668 + DvMF_2021 0.82 +0.2 2,503,668 + DvMF_2021 0.82 +2.0 2,504,084 - -1.1 2,504,114 - -2.6 2,504,134 - -4.3 2,504,335 - -0.9 2,504,351 - -0.5 2,504,359 - +1.2 2,504,830 + DvMF_2022 0.36 -1.0
Or see this region's nucleotide sequence