Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2018 and DvMF_2019 are separated by 271 nucleotides DvMF_2019 and DvMF_2020 overlap by 4 nucleotides DvMF_2020 and DvMF_2021 are separated by 128 nucleotides
DvMF_2018: DvMF_2018 - hypothetical protein (RefSeq), at 2,499,434 to 2,500,591
_2018
DvMF_2019: DvMF_2019 - dihydrouridine synthase DuS (RefSeq), at 2,500,863 to 2,501,918
_2019
DvMF_2020: DvMF_2020 - hydroxymethylbutenyl pyrophosphate reductase (RefSeq), at 2,501,915 to 2,502,760
_2020
DvMF_2021: DvMF_2021 - response regulator receiver modulated CheW protein (RefSeq), at 2,502,889 to 2,503,839
_2021
Position (kb)
2500
2501
2502 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2499.962 kb on + strand, within DvMF_2018 at 2500.050 kb on - strand, within DvMF_2018 at 2500.050 kb on - strand, within DvMF_2018 at 2500.050 kb on - strand, within DvMF_2018 at 2500.196 kb on + strand, within DvMF_2018 at 2500.366 kb on + strand, within DvMF_2018 at 2500.396 kb on - strand, within DvMF_2018 at 2500.424 kb on + strand, within DvMF_2018 at 2500.683 kb on - strand at 2500.769 kb on + strand at 2501.028 kb on - strand, within DvMF_2019 at 2501.105 kb on + strand, within DvMF_2019 at 2501.113 kb on - strand, within DvMF_2019 at 2501.113 kb on - strand, within DvMF_2019 at 2501.178 kb on - strand, within DvMF_2019 at 2501.206 kb on - strand, within DvMF_2019 at 2501.207 kb on + strand, within DvMF_2019 at 2501.456 kb on - strand, within DvMF_2019 at 2501.501 kb on + strand, within DvMF_2019 at 2501.544 kb on + strand, within DvMF_2019 at 2501.544 kb on + strand, within DvMF_2019 at 2501.685 kb on - strand, within DvMF_2019 at 2501.742 kb on - strand, within DvMF_2019
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 2,499,962 + DvMF_2018 0.46 -0.4 2,500,050 - DvMF_2018 0.53 -0.4 2,500,050 - DvMF_2018 0.53 -0.0 2,500,050 - DvMF_2018 0.53 -0.9 2,500,196 + DvMF_2018 0.66 -0.3 2,500,366 + DvMF_2018 0.80 -0.7 2,500,396 - DvMF_2018 0.83 -3.5 2,500,424 + DvMF_2018 0.85 -0.6 2,500,683 - -0.5 2,500,769 + -0.3 2,501,028 - DvMF_2019 0.16 +0.2 2,501,105 + DvMF_2019 0.23 +0.2 2,501,113 - DvMF_2019 0.24 +1.3 2,501,113 - DvMF_2019 0.24 +0.2 2,501,178 - DvMF_2019 0.30 +0.3 2,501,206 - DvMF_2019 0.32 +0.4 2,501,207 + DvMF_2019 0.33 +0.0 2,501,456 - DvMF_2019 0.56 +0.7 2,501,501 + DvMF_2019 0.60 -0.8 2,501,544 + DvMF_2019 0.64 -0.3 2,501,544 + DvMF_2019 0.64 -0.0 2,501,685 - DvMF_2019 0.78 -0.7 2,501,742 - DvMF_2019 0.83 +0.3
Or see this region's nucleotide sequence