Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1468 and DvMF_1469 are separated by 110 nucleotides DvMF_1469 and DvMF_1470 are separated by 285 nucleotides DvMF_1470 and DvMF_1471 overlap by 4 nucleotides
DvMF_1468: DvMF_1468 - adenylosuccinate lyase (RefSeq), at 1,798,933 to 1,800,231
_1468
DvMF_1469: DvMF_1469 - orotate phosphoribosyltransferase (RefSeq), at 1,800,342 to 1,800,902
_1469
DvMF_1470: DvMF_1470 - ErfK/YbiS/YcfS/YnhG family protein (RefSeq), at 1,801,188 to 1,802,486
_1470
DvMF_1471: DvMF_1471 - hypothetical protein (RefSeq), at 1,802,483 to 1,803,349
_1471
Position (kb)
1801
1802
1803 Strain fitness (log2 ratio)
-1
0
1 at 1800.285 kb on - strand at 1800.788 kb on + strand, within DvMF_1469 at 1800.881 kb on + strand at 1800.923 kb on + strand at 1800.982 kb on + strand at 1801.055 kb on - strand at 1801.152 kb on + strand at 1801.792 kb on - strand, within DvMF_1470 at 1801.803 kb on - strand, within DvMF_1470 at 1801.810 kb on - strand, within DvMF_1470 at 1801.810 kb on - strand, within DvMF_1470 at 1802.243 kb on + strand, within DvMF_1470 at 1802.409 kb on + strand at 1802.610 kb on + strand, within DvMF_1471 at 1802.697 kb on - strand, within DvMF_1471 at 1803.228 kb on + strand, within DvMF_1471
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 1,800,285 - +0.8 1,800,788 + DvMF_1469 0.80 +0.3 1,800,881 + +0.8 1,800,923 + +0.6 1,800,982 + +0.2 1,801,055 - -0.6 1,801,152 + +0.6 1,801,792 - DvMF_1470 0.46 -0.4 1,801,803 - DvMF_1470 0.47 -0.4 1,801,810 - DvMF_1470 0.48 -0.1 1,801,810 - DvMF_1470 0.48 +0.3 1,802,243 + DvMF_1470 0.81 +0.6 1,802,409 + +0.5 1,802,610 + DvMF_1471 0.15 +0.1 1,802,697 - DvMF_1471 0.25 -1.4 1,803,228 + DvMF_1471 0.86 +0.6
Or see this region's nucleotide sequence