Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0225 and DvMF_0226 are separated by 315 nucleotides DvMF_0226 and DvMF_0227 are separated by 242 nucleotides DvMF_0227 and DvMF_0228 are separated by 215 nucleotides DvMF_0228 and DvMF_0229 are separated by 45 nucleotides
DvMF_0225: DvMF_0225 - glycine cleavage system protein H (RefSeq), at 252,596 to 252,979
_0225
DvMF_0226: DvMF_0226 - response regulator receiver protein (RefSeq), at 253,295 to 253,708
_0226
DvMF_0227: DvMF_0227 - phosphoglucomutase (RefSeq), at 253,951 to 255,600
_0227
DvMF_0228: DvMF_0228 - hypothetical protein (RefSeq), at 255,816 to 256,010
_0228
DvMF_0229: DvMF_0229 - DNA methylase N-4/N-6 domain protein (RefSeq), at 256,056 to 257,255
_0229
Position (kb) 253
254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 252.995 kb on + strand at 253.061 kb on + strand at 253.082 kb on - strand at 253.112 kb on - strand at 253.152 kb on - strand at 253.252 kb on + strand at 253.666 kb on + strand, within DvMF_0226 at 253.833 kb on + strand at 253.908 kb on + strand at 253.995 kb on - strand at 254.152 kb on - strand, within DvMF_0227 at 254.268 kb on - strand, within DvMF_0227 at 254.305 kb on - strand, within DvMF_0227 at 254.388 kb on - strand, within DvMF_0227 at 254.476 kb on + strand, within DvMF_0227 at 254.484 kb on - strand, within DvMF_0227 at 255.144 kb on + strand, within DvMF_0227 at 255.157 kb on + strand, within DvMF_0227 at 255.262 kb on - strand, within DvMF_0227 at 255.272 kb on + strand, within DvMF_0227 at 255.518 kb on + strand at 255.609 kb on - strand at 255.620 kb on - strand at 255.723 kb on - strand at 255.880 kb on - strand, within DvMF_0228
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 252,995 + -0.2 253,061 + +0.0 253,082 - -0.5 253,112 - -2.0 253,152 - -0.2 253,252 + -1.0 253,666 + DvMF_0226 0.90 -2.1 253,833 + -1.0 253,908 + -0.9 253,995 - -0.7 254,152 - DvMF_0227 0.12 +0.1 254,268 - DvMF_0227 0.19 +0.4 254,305 - DvMF_0227 0.21 +0.1 254,388 - DvMF_0227 0.26 +0.6 254,476 + DvMF_0227 0.32 -3.0 254,484 - DvMF_0227 0.32 -1.1 255,144 + DvMF_0227 0.72 -2.6 255,157 + DvMF_0227 0.73 -0.6 255,262 - DvMF_0227 0.79 +0.6 255,272 + DvMF_0227 0.80 -0.2 255,518 + -1.1 255,609 - -0.6 255,620 - -0.4 255,723 - -0.5 255,880 - DvMF_0228 0.33 -1.7
Or see this region's nucleotide sequence