Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0207 and DvMF_0208 are separated by 274 nucleotides DvMF_0208 and DvMF_0209 are separated by 30 nucleotides
DvMF_0207: DvMF_0207 - thiamine biosynthesis protein ThiC (RefSeq), at 225,567 to 226,856
_0207
DvMF_0208: DvMF_0208 - EAL domain protein (RefSeq), at 227,131 to 229,419
_0208
DvMF_0209: DvMF_0209 - D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding (RefSeq), at 229,450 to 230,418
_0209
Position (kb) 227
228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 226.330 kb on + strand, within DvMF_0207 at 226.340 kb on + strand, within DvMF_0207 at 226.355 kb on + strand, within DvMF_0207 at 226.578 kb on - strand, within DvMF_0207 at 226.720 kb on + strand, within DvMF_0207 at 226.851 kb on + strand at 226.880 kb on - strand at 227.061 kb on + strand at 227.297 kb on - strand at 227.306 kb on - strand at 227.364 kb on - strand, within DvMF_0208 at 227.463 kb on - strand, within DvMF_0208 at 227.555 kb on - strand, within DvMF_0208 at 227.660 kb on + strand, within DvMF_0208 at 227.913 kb on + strand, within DvMF_0208 at 228.036 kb on + strand, within DvMF_0208 at 228.036 kb on + strand, within DvMF_0208 at 228.180 kb on + strand, within DvMF_0208 at 228.329 kb on - strand, within DvMF_0208 at 228.405 kb on + strand, within DvMF_0208 at 228.526 kb on - strand, within DvMF_0208 at 228.578 kb on - strand, within DvMF_0208 at 228.720 kb on - strand, within DvMF_0208 at 229.152 kb on - strand, within DvMF_0208 at 229.191 kb on + strand at 229.199 kb on - strand at 229.342 kb on + strand at 229.455 kb on - strand at 229.525 kb on + strand at 229.543 kb on + strand at 229.590 kb on - strand, within DvMF_0209 at 229.737 kb on + strand, within DvMF_0209 at 229.766 kb on + strand, within DvMF_0209 at 229.806 kb on - strand, within DvMF_0209 at 230.125 kb on + strand, within DvMF_0209 at 230.396 kb on - strand at 230.406 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 226,330 + DvMF_0207 0.59 -1.8 226,340 + DvMF_0207 0.60 -1.0 226,355 + DvMF_0207 0.61 -1.6 226,578 - DvMF_0207 0.78 -1.4 226,720 + DvMF_0207 0.89 -1.1 226,851 + -1.3 226,880 - -1.3 227,061 + -1.0 227,297 - -0.7 227,306 - -3.4 227,364 - DvMF_0208 0.10 -0.9 227,463 - DvMF_0208 0.15 -3.4 227,555 - DvMF_0208 0.19 -1.2 227,660 + DvMF_0208 0.23 -1.2 227,913 + DvMF_0208 0.34 -3.2 228,036 + DvMF_0208 0.40 -1.3 228,036 + DvMF_0208 0.40 -1.9 228,180 + DvMF_0208 0.46 +1.8 228,329 - DvMF_0208 0.52 -3.0 228,405 + DvMF_0208 0.56 -1.5 228,526 - DvMF_0208 0.61 -2.1 228,578 - DvMF_0208 0.63 -0.1 228,720 - DvMF_0208 0.69 -0.8 229,152 - DvMF_0208 0.88 -2.3 229,191 + -1.7 229,199 - +1.6 229,342 + +0.8 229,455 - -0.4 229,525 + +0.2 229,543 + -0.2 229,590 - DvMF_0209 0.14 +0.0 229,737 + DvMF_0209 0.30 -0.9 229,766 + DvMF_0209 0.33 -0.6 229,806 - DvMF_0209 0.37 -0.7 230,125 + DvMF_0209 0.70 -0.8 230,396 - -0.4 230,406 - -0.6
Or see this region's nucleotide sequence