Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0061 and DvMF_0062 are separated by 33 nucleotides DvMF_0062 and DvMF_0063 are separated by 158 nucleotides DvMF_0063 and DvMF_0064 overlap by 4 nucleotides DvMF_0064 and DvMF_0065 are separated by 501 nucleotides
DvMF_0061: DvMF_0061 - primosomal protein N' (RefSeq), at 79,119 to 81,539
_0061
DvMF_0062: DvMF_0062 - hypothetical protein (RefSeq), at 81,573 to 81,740
_0062
DvMF_0063: DvMF_0063 - response regulator receiver protein (RefSeq), at 81,899 to 82,291
_0063
DvMF_0064: DvMF_0064 - histidine kinase (RefSeq), at 82,288 to 84,084
_0064
DvMF_0065: DvMF_0065 - membrane protein involved in aromatic hydrocarbon degradation (RefSeq), at 84,586 to 85,872
_0065
Position (kb)
82
83
84
85 Strain fitness (log2 ratio)
-1
0
1
2 at 81.610 kb on + strand, within DvMF_0062 at 81.671 kb on - strand, within DvMF_0062 at 81.896 kb on + strand at 82.023 kb on - strand, within DvMF_0063 at 82.325 kb on + strand at 82.484 kb on + strand, within DvMF_0064 at 82.538 kb on + strand, within DvMF_0064 at 82.692 kb on - strand, within DvMF_0064 at 82.741 kb on - strand, within DvMF_0064 at 82.790 kb on + strand, within DvMF_0064 at 82.837 kb on + strand, within DvMF_0064 at 83.051 kb on + strand, within DvMF_0064 at 83.137 kb on + strand, within DvMF_0064 at 83.191 kb on + strand, within DvMF_0064 at 83.565 kb on - strand, within DvMF_0064 at 83.875 kb on + strand, within DvMF_0064 at 84.197 kb on + strand at 84.500 kb on + strand at 84.662 kb on - strand at 84.702 kb on + strand at 84.786 kb on + strand, within DvMF_0065 at 84.786 kb on + strand, within DvMF_0065 at 84.984 kb on + strand, within DvMF_0065 at 84.992 kb on - strand, within DvMF_0065
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 81,610 + DvMF_0062 0.22 -0.2 81,671 - DvMF_0062 0.58 -1.0 81,896 + +0.1 82,023 - DvMF_0063 0.32 +0.7 82,325 + +0.3 82,484 + DvMF_0064 0.11 +0.3 82,538 + DvMF_0064 0.14 +0.6 82,692 - DvMF_0064 0.22 -0.2 82,741 - DvMF_0064 0.25 +0.4 82,790 + DvMF_0064 0.28 -0.5 82,837 + DvMF_0064 0.31 +0.1 83,051 + DvMF_0064 0.42 -0.2 83,137 + DvMF_0064 0.47 +0.1 83,191 + DvMF_0064 0.50 +0.1 83,565 - DvMF_0064 0.71 -0.7 83,875 + DvMF_0064 0.88 -0.3 84,197 + +0.9 84,500 + +0.6 84,662 - +1.0 84,702 + +2.3 84,786 + DvMF_0065 0.16 +1.8 84,786 + DvMF_0065 0.16 +1.9 84,984 + DvMF_0065 0.31 +2.1 84,992 - DvMF_0065 0.32 +1.3
Or see this region's nucleotide sequence