Experiment: MoYLS4 with Gentamicin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0048 and DvMF_0049 are separated by 264 nucleotides DvMF_0049 and DvMF_0050 are separated by 278 nucleotides DvMF_0050 and DvMF_0051 are separated by 226 nucleotides
DvMF_0048: DvMF_0048 - argininosuccinate lyase (RefSeq), at 63,998 to 65,380
_0048
DvMF_0049: DvMF_0049 - hypothetical protein (RefSeq), at 65,645 to 66,274
_0049
DvMF_0050: DvMF_0050 - RNP-1 like RNA-binding protein (RefSeq), at 66,553 to 66,819
_0050
DvMF_0051: DvMF_0051 - extracellular solute-binding protein family 3 (RefSeq), at 67,046 to 67,840
_0051
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1 at 64.646 kb on + strand, within DvMF_0048 at 64.852 kb on + strand, within DvMF_0048 at 64.972 kb on + strand, within DvMF_0048 at 65.213 kb on + strand, within DvMF_0048 at 65.331 kb on + strand at 65.507 kb on - strand at 65.667 kb on + strand at 65.700 kb on - strand at 65.818 kb on + strand, within DvMF_0049 at 65.904 kb on + strand, within DvMF_0049 at 66.116 kb on + strand, within DvMF_0049 at 66.136 kb on + strand, within DvMF_0049 at 66.136 kb on + strand, within DvMF_0049 at 66.271 kb on - strand at 66.439 kb on + strand at 66.500 kb on - strand at 67.057 kb on + strand at 67.072 kb on + strand at 67.211 kb on - strand, within DvMF_0051 at 67.211 kb on - strand, within DvMF_0051
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Gentamicin 0.0625 mg/ml remove 64,646 + DvMF_0048 0.47 -0.8 64,852 + DvMF_0048 0.62 -0.2 64,972 + DvMF_0048 0.70 -0.3 65,213 + DvMF_0048 0.88 +0.1 65,331 + -0.4 65,507 - -0.4 65,667 + -1.2 65,700 - -1.0 65,818 + DvMF_0049 0.27 +0.8 65,904 + DvMF_0049 0.41 +0.4 66,116 + DvMF_0049 0.75 -0.3 66,136 + DvMF_0049 0.78 -0.7 66,136 + DvMF_0049 0.78 -0.5 66,271 - -1.3 66,439 + +0.4 66,500 - -0.6 67,057 + -0.5 67,072 + -0.8 67,211 - DvMF_0051 0.21 -0.1 67,211 - DvMF_0051 0.21 -1.0
Or see this region's nucleotide sequence