Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt upp and Shewana3_2545 are separated by 327 nucleotides Shewana3_2545 and purN overlap by 1 nucleotides purN and Shewana3_2547 are separated by 138 nucleotides
Shewana3_2544: upp - uracil phosphoribosyltransferase (RefSeq), at 3,041,754 to 3,042,380
upp
Shewana3_2545: Shewana3_2545 - phosphoribosylaminoimidazole synthetase (RefSeq), at 3,042,708 to 3,043,745
_2545
Shewana3_2546: purN - phosphoribosylglycinamide formyltransferase (RefSeq), at 3,043,745 to 3,044,389
purN
Shewana3_2547: Shewana3_2547 - UMP phosphatase (RefSeq), at 3,044,528 to 3,045,274
_2547
Position (kb)
3042
3043
3044 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3041.795 kb on - strand at 3041.795 kb on - strand at 3041.795 kb on - strand at 3041.795 kb on - strand at 3041.809 kb on - strand at 3041.852 kb on - strand, within upp at 3041.852 kb on - strand, within upp at 3041.878 kb on + strand, within upp at 3041.924 kb on + strand, within upp at 3042.060 kb on - strand, within upp at 3042.103 kb on + strand, within upp at 3042.357 kb on - strand at 3042.568 kb on - strand at 3042.658 kb on + strand at 3042.715 kb on + strand at 3042.715 kb on + strand at 3042.723 kb on - strand at 3042.819 kb on - strand, within Shewana3_2545 at 3043.003 kb on + strand, within Shewana3_2545 at 3043.696 kb on + strand at 3043.725 kb on + strand at 3043.756 kb on - strand at 3043.758 kb on + strand at 3043.826 kb on + strand, within purN at 3044.089 kb on + strand, within purN at 3044.172 kb on + strand, within purN at 3044.278 kb on + strand, within purN at 3044.307 kb on + strand, within purN at 3044.315 kb on - strand, within purN at 3044.675 kb on - strand, within Shewana3_2547
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 3,041,795 - +0.3 3,041,795 - -0.1 3,041,795 - -0.2 3,041,795 - +0.5 3,041,809 - +0.8 3,041,852 - upp Shewana3_2544 0.16 -0.1 3,041,852 - upp Shewana3_2544 0.16 +0.2 3,041,878 + upp Shewana3_2544 0.20 +1.0 3,041,924 + upp Shewana3_2544 0.27 +1.1 3,042,060 - upp Shewana3_2544 0.49 -0.5 3,042,103 + upp Shewana3_2544 0.56 -0.8 3,042,357 - -0.1 3,042,568 - -0.4 3,042,658 + -3.0 3,042,715 + -1.9 3,042,715 + -1.1 3,042,723 - -1.1 3,042,819 - Shewana3_2545 0.11 -1.1 3,043,003 + Shewana3_2545 0.28 -5.1 3,043,696 + -1.4 3,043,725 + -4.0 3,043,756 - -5.4 3,043,758 + -3.1 3,043,826 + purN Shewana3_2546 0.13 -4.4 3,044,089 + purN Shewana3_2546 0.53 -3.2 3,044,172 + purN Shewana3_2546 0.66 -5.0 3,044,278 + purN Shewana3_2546 0.83 -2.6 3,044,307 + purN Shewana3_2546 0.87 -0.9 3,044,315 - purN Shewana3_2546 0.88 -2.0 3,044,675 - Shewana3_2547 0.20 -0.6
Or see this region's nucleotide sequence