Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1120 and surE overlap by 4 nucleotides surE and Shewana3_1122 overlap by 4 nucleotides Shewana3_1122 and Shewana3_1123 are separated by 84 nucleotides
Shewana3_1120: Shewana3_1120 - tRNA pseudouridine synthase D, TruD (RefSeq), at 1,318,767 to 1,319,894
_1120
Shewana3_1121: surE - stationary phase survival protein SurE (RefSeq), at 1,319,891 to 1,320,640
surE
Shewana3_1122: Shewana3_1122 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,320,637 to 1,321,272
_1122
Shewana3_1123: Shewana3_1123 - peptidase M23B (RefSeq), at 1,321,357 to 1,322,253
_1123
Position (kb)
1319
1320
1321 Strain fitness (log2 ratio)
-2
-1
0
1 at 1318.941 kb on - strand, within Shewana3_1120 at 1319.026 kb on - strand, within Shewana3_1120 at 1319.066 kb on + strand, within Shewana3_1120 at 1319.074 kb on - strand, within Shewana3_1120 at 1319.146 kb on + strand, within Shewana3_1120 at 1319.262 kb on + strand, within Shewana3_1120 at 1319.331 kb on + strand, within Shewana3_1120 at 1319.333 kb on + strand, within Shewana3_1120 at 1319.350 kb on + strand, within Shewana3_1120 at 1319.584 kb on + strand, within Shewana3_1120 at 1319.876 kb on + strand at 1319.886 kb on + strand at 1319.978 kb on + strand, within surE at 1320.132 kb on - strand, within surE at 1320.232 kb on + strand, within surE at 1320.397 kb on + strand, within surE at 1320.433 kb on + strand, within surE at 1320.441 kb on - strand, within surE at 1320.578 kb on + strand at 1320.728 kb on + strand, within Shewana3_1122 at 1320.950 kb on + strand, within Shewana3_1122 at 1321.345 kb on - strand at 1321.439 kb on - strand at 1321.544 kb on - strand, within Shewana3_1123
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 1,318,941 - Shewana3_1120 0.15 -2.3 1,319,026 - Shewana3_1120 0.23 -2.4 1,319,066 + Shewana3_1120 0.27 +0.2 1,319,074 - Shewana3_1120 0.27 -1.6 1,319,146 + Shewana3_1120 0.34 -1.0 1,319,262 + Shewana3_1120 0.44 -0.6 1,319,331 + Shewana3_1120 0.50 -0.9 1,319,333 + Shewana3_1120 0.50 +0.1 1,319,350 + Shewana3_1120 0.52 -0.5 1,319,584 + Shewana3_1120 0.72 -0.3 1,319,876 + -0.6 1,319,886 + -1.9 1,319,978 + surE Shewana3_1121 0.12 -0.3 1,320,132 - surE Shewana3_1121 0.32 -1.2 1,320,232 + surE Shewana3_1121 0.45 -1.5 1,320,397 + surE Shewana3_1121 0.67 -0.5 1,320,433 + surE Shewana3_1121 0.72 -0.8 1,320,441 - surE Shewana3_1121 0.73 -2.0 1,320,578 + -1.8 1,320,728 + Shewana3_1122 0.14 -0.3 1,320,950 + Shewana3_1122 0.49 +0.1 1,321,345 - -0.3 1,321,439 - +0.3 1,321,544 - Shewana3_1123 0.21 -0.5
Or see this region's nucleotide sequence