Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lipB and Shewana3_0990 are separated by 92 nucleotides Shewana3_0990 and Shewana3_0991 are separated by 121 nucleotides Shewana3_0991 and Shewana3_0992 are separated by 144 nucleotides Shewana3_0992 and Shewana3_0993 overlap by 17 nucleotides
Shewana3_0989: lipB - lipoyltransferase (RefSeq), at 1,159,865 to 1,160,518
lipB
Shewana3_0990: Shewana3_0990 - hypothetical protein (RefSeq), at 1,160,611 to 1,160,877
_0990
Shewana3_0991: Shewana3_0991 - penicillin-binding protein 6 (RefSeq), at 1,160,999 to 1,162,174
_0991
Shewana3_0992: Shewana3_0992 - rare lipoprotein A (RefSeq), at 1,162,319 to 1,163,161
_0992
Shewana3_0993: Shewana3_0993 - lytic murein transglycosylase B (RefSeq), at 1,163,145 to 1,164,209
_0993
Position (kb)
1160
1161
1162
1163 Strain fitness (log2 ratio)
-1
0
1 at 1161.637 kb on - strand, within Shewana3_0991 at 1162.240 kb on - strand at 1162.310 kb on - strand at 1162.404 kb on - strand, within Shewana3_0992 at 1162.438 kb on + strand, within Shewana3_0992 at 1162.503 kb on - strand, within Shewana3_0992 at 1162.580 kb on - strand, within Shewana3_0992 at 1162.605 kb on + strand, within Shewana3_0992 at 1162.605 kb on + strand, within Shewana3_0992 at 1162.801 kb on - strand, within Shewana3_0992 at 1162.902 kb on + strand, within Shewana3_0992 at 1162.910 kb on - strand, within Shewana3_0992 at 1162.910 kb on - strand, within Shewana3_0992
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 1,161,637 - Shewana3_0991 0.54 -1.0 1,162,240 - +0.4 1,162,310 - -0.2 1,162,404 - Shewana3_0992 0.10 +0.8 1,162,438 + Shewana3_0992 0.14 -0.8 1,162,503 - Shewana3_0992 0.22 +0.1 1,162,580 - Shewana3_0992 0.31 +0.8 1,162,605 + Shewana3_0992 0.34 -0.8 1,162,605 + Shewana3_0992 0.34 -0.2 1,162,801 - Shewana3_0992 0.57 +0.4 1,162,902 + Shewana3_0992 0.69 -0.0 1,162,910 - Shewana3_0992 0.70 -0.0 1,162,910 - Shewana3_0992 0.70 +0.3
Or see this region's nucleotide sequence