Experiment: pyruvate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2925 and Shew_2926 are separated by 27 nucleotides Shew_2926 and Shew_2927 are separated by 26 nucleotides Shew_2927 and Shew_2928 are separated by 146 nucleotides Shew_2928 and leuS are separated by 121 nucleotides
Shew_2925: Shew_2925 - putative metalloprotease (RefSeq), at 3,464,142 to 3,464,615
_2925
Shew_2926: Shew_2926 - CBS domain-containing protein (RefSeq), at 3,464,643 to 3,465,518
_2926
Shew_2927: Shew_2927 - apolipoprotein N-acyltransferase (RefSeq), at 3,465,545 to 3,467,158
_2927
Shew_2928: Shew_2928 - hypothetical protein (RefSeq), at 3,467,305 to 3,467,805
_2928
Shew_2929: leuS - leucyl-tRNA synthetase (RefSeq), at 3,467,927 to 3,470,518
leuS
Position (kb) 3465
3466
3467
3468 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 3464.761 kb on + strand, within Shew_2926 at 3465.272 kb on + strand, within Shew_2926 at 3465.475 kb on + strand at 3467.293 kb on + strand at 3467.405 kb on + strand, within Shew_2928 at 3467.553 kb on + strand, within Shew_2928 at 3467.553 kb on + strand, within Shew_2928 at 3467.555 kb on + strand, within Shew_2928 at 3467.864 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate (C) remove 3,464,761 + Shew_2926 0.13 -2.8 3,465,272 + Shew_2926 0.72 -4.8 3,465,475 + -0.6 3,467,293 + -1.2 3,467,405 + Shew_2928 0.20 +0.3 3,467,553 + Shew_2928 0.50 +0.4 3,467,553 + Shew_2928 0.50 +0.7 3,467,555 + Shew_2928 0.50 +0.6 3,467,864 + +3.8
Or see this region's nucleotide sequence