Experiment: pyruvate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0517 and Shew_0518 are separated by 63 nucleotides Shew_0518 and Shew_0519 are separated by 78 nucleotides Shew_0519 and Shew_0520 are separated by 224 nucleotides
Shew_0517: Shew_0517 - putative plasmid transfer protein (RefSeq), at 586,558 to 587,754
_0517
Shew_0518: Shew_0518 - hypothetical protein (RefSeq), at 587,818 to 588,558
_0518
Shew_0519: Shew_0519 - transcriptional repressor protein MetJ (RefSeq), at 588,637 to 588,951
_0519
Shew_0520: Shew_0520 - O-succinylhomoserine (thiol)-lyase (RefSeq), at 589,176 to 590,342
_0520
Position (kb) 587
588
589 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 586.966 kb on + strand, within Shew_0517 at 587.067 kb on - strand, within Shew_0517 at 587.146 kb on - strand, within Shew_0517 at 587.231 kb on + strand, within Shew_0517 at 587.357 kb on + strand, within Shew_0517 at 587.375 kb on - strand, within Shew_0517 at 587.505 kb on - strand, within Shew_0517 at 587.555 kb on - strand, within Shew_0517 at 587.810 kb on - strand at 587.875 kb on - strand at 587.944 kb on - strand, within Shew_0518 at 587.944 kb on - strand, within Shew_0518 at 587.975 kb on - strand, within Shew_0518 at 587.985 kb on + strand, within Shew_0518 at 588.520 kb on - strand at 588.541 kb on + strand at 588.809 kb on + strand, within Shew_0519 at 588.955 kb on - strand at 588.955 kb on - strand at 589.201 kb on + strand at 589.444 kb on - strand, within Shew_0520
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate (C) remove 586,966 + Shew_0517 0.34 +0.1 587,067 - Shew_0517 0.43 +0.1 587,146 - Shew_0517 0.49 -1.4 587,231 + Shew_0517 0.56 -0.1 587,357 + Shew_0517 0.67 +0.9 587,375 - Shew_0517 0.68 -0.3 587,505 - Shew_0517 0.79 +0.2 587,555 - Shew_0517 0.83 -0.7 587,810 - -0.3 587,875 - -0.6 587,944 - Shew_0518 0.17 -0.8 587,944 - Shew_0518 0.17 -1.8 587,975 - Shew_0518 0.21 -0.8 587,985 + Shew_0518 0.23 -1.6 588,520 - -1.8 588,541 + -0.9 588,809 + Shew_0519 0.55 -1.1 588,955 - -1.1 588,955 - -2.4 589,201 + -2.8 589,444 - Shew_0520 0.23 -5.8
Or see this region's nucleotide sequence