Experiment: R2A with Manganese (II) chloride tetrahydrate 6.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS11390 and LRK54_RS11395 are separated by 885 nucleotides LRK54_RS11395 and LRK54_RS11400 overlap by 4 nucleotides LRK54_RS11400 and LRK54_RS11405 are separated by 12 nucleotides
LRK54_RS11390: LRK54_RS11390 - ribulose-phosphate 3-epimerase, at 2,522,998 to 2,523,666
_RS11390
LRK54_RS11395: LRK54_RS11395 - proteobacterial dedicated sortase system response regulator, at 2,524,552 to 2,525,241
_RS11395
LRK54_RS11400: LRK54_RS11400 - hypothetical protein, at 2,525,238 to 2,525,819
_RS11400
LRK54_RS11405: LRK54_RS11405 - ATP-binding protein, at 2,525,832 to 2,527,847
_RS11405
Position (kb)
2524
2525
2526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2523.812 kb on + strand at 2523.812 kb on + strand at 2523.812 kb on + strand at 2523.812 kb on + strand at 2523.812 kb on + strand at 2523.813 kb on - strand at 2523.813 kb on - strand at 2523.813 kb on - strand at 2523.813 kb on - strand at 2523.969 kb on + strand at 2523.969 kb on + strand at 2523.969 kb on + strand at 2523.970 kb on - strand at 2523.970 kb on - strand at 2523.970 kb on - strand at 2523.970 kb on - strand at 2523.970 kb on - strand at 2523.970 kb on - strand at 2523.970 kb on - strand at 2523.970 kb on - strand at 2523.970 kb on - strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.117 kb on + strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.118 kb on - strand at 2524.464 kb on + strand at 2524.465 kb on - strand at 2524.502 kb on + strand at 2524.502 kb on + strand at 2524.502 kb on + strand at 2524.503 kb on - strand at 2524.503 kb on - strand at 2524.503 kb on - strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.517 kb on + strand at 2524.518 kb on - strand at 2524.518 kb on - strand at 2524.518 kb on - strand at 2524.518 kb on - strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.603 kb on + strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.604 kb on - strand at 2524.630 kb on + strand, within LRK54_RS11395 at 2524.645 kb on + strand, within LRK54_RS11395 at 2524.646 kb on - strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.855 kb on + strand, within LRK54_RS11395 at 2524.856 kb on - strand, within LRK54_RS11395 at 2524.856 kb on - strand, within LRK54_RS11395 at 2524.856 kb on - strand, within LRK54_RS11395 at 2524.856 kb on - strand, within LRK54_RS11395 at 2524.856 kb on - strand, within LRK54_RS11395 at 2524.856 kb on - strand, within LRK54_RS11395 at 2524.856 kb on - strand, within LRK54_RS11395 at 2524.856 kb on - strand, within LRK54_RS11395 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.406 kb on + strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.407 kb on - strand, within LRK54_RS11400 at 2525.439 kb on + strand, within LRK54_RS11400 at 2525.439 kb on + strand, within LRK54_RS11400 at 2525.439 kb on + strand, within LRK54_RS11400 at 2525.439 kb on + strand, within LRK54_RS11400 at 2525.439 kb on + strand, within LRK54_RS11400 at 2525.439 kb on + strand, within LRK54_RS11400 at 2525.439 kb on + strand, within LRK54_RS11400 at 2525.440 kb on - strand, within LRK54_RS11400 at 2525.440 kb on - strand, within LRK54_RS11400 at 2525.440 kb on - strand, within LRK54_RS11400 at 2525.544 kb on + strand, within LRK54_RS11400 at 2525.544 kb on + strand, within LRK54_RS11400 at 2525.573 kb on - strand, within LRK54_RS11400 at 2525.622 kb on + strand, within LRK54_RS11400 at 2525.622 kb on + strand, within LRK54_RS11400 at 2525.623 kb on - strand, within LRK54_RS11400 at 2525.623 kb on - strand, within LRK54_RS11400 at 2525.623 kb on - strand, within LRK54_RS11400 at 2525.623 kb on - strand, within LRK54_RS11400 at 2525.623 kb on - strand, within LRK54_RS11400 at 2525.623 kb on - strand, within LRK54_RS11400 at 2525.623 kb on - strand, within LRK54_RS11400 at 2525.623 kb on - strand, within LRK54_RS11400 at 2525.679 kb on - strand, within LRK54_RS11400 at 2525.679 kb on - strand, within LRK54_RS11400 at 2525.901 kb on + strand at 2525.901 kb on + strand at 2525.901 kb on + strand at 2525.901 kb on + strand at 2525.901 kb on + strand at 2525.901 kb on + strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2525.902 kb on - strand at 2526.027 kb on + strand at 2526.027 kb on + strand at 2526.027 kb on + strand at 2526.027 kb on + strand at 2526.027 kb on + strand at 2526.027 kb on + strand at 2526.027 kb on + strand at 2526.027 kb on + strand at 2526.027 kb on + strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.028 kb on - strand at 2526.067 kb on - strand, within LRK54_RS11405 at 2526.162 kb on + strand, within LRK54_RS11405 at 2526.162 kb on + strand, within LRK54_RS11405 at 2526.162 kb on + strand, within LRK54_RS11405 at 2526.162 kb on + strand, within LRK54_RS11405 at 2526.163 kb on - strand, within LRK54_RS11405 at 2526.163 kb on - strand, within LRK54_RS11405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 6.25 mM remove 2,523,812 + +0.7 2,523,812 + +1.0 2,523,812 + +1.4 2,523,812 + +0.2 2,523,812 + +1.4 2,523,813 - +1.3 2,523,813 - +0.3 2,523,813 - +0.1 2,523,813 - +0.8 2,523,969 + +2.2 2,523,969 + +2.8 2,523,969 + +2.4 2,523,970 - +1.3 2,523,970 - +1.6 2,523,970 - +2.0 2,523,970 - +1.7 2,523,970 - +0.1 2,523,970 - +0.1 2,523,970 - -0.1 2,523,970 - +2.7 2,523,970 - +1.6 2,524,117 + +0.4 2,524,117 + -0.1 2,524,117 + -1.2 2,524,117 + +0.8 2,524,117 + +2.3 2,524,117 + -0.5 2,524,117 + +0.9 2,524,117 + +0.3 2,524,117 + +0.2 2,524,117 + -0.4 2,524,117 + -0.2 2,524,117 + +0.1 2,524,117 + -0.1 2,524,118 - -0.4 2,524,118 - +0.8 2,524,118 - +1.9 2,524,118 - -0.5 2,524,118 - -0.0 2,524,118 - -1.4 2,524,118 - -1.0 2,524,118 - +0.4 2,524,118 - +0.4 2,524,118 - -1.2 2,524,118 - +1.9 2,524,464 + +1.4 2,524,465 - -1.0 2,524,502 + +0.1 2,524,502 + +0.2 2,524,502 + +0.0 2,524,503 - -3.0 2,524,503 - -2.7 2,524,503 - +0.7 2,524,517 + -2.2 2,524,517 + -1.9 2,524,517 + +0.5 2,524,517 + -0.3 2,524,517 + -3.0 2,524,517 + -0.1 2,524,517 + -1.6 2,524,517 + -0.9 2,524,517 + -1.7 2,524,517 + +1.8 2,524,517 + -1.9 2,524,517 + -2.2 2,524,518 - -0.2 2,524,518 - -0.4 2,524,518 - -0.2 2,524,518 - -0.2 2,524,603 + -1.6 2,524,603 + -0.1 2,524,603 + -0.4 2,524,603 + +0.3 2,524,603 + -0.6 2,524,603 + -1.3 2,524,603 + -2.2 2,524,603 + -0.3 2,524,603 + -0.8 2,524,603 + +1.4 2,524,603 + -1.7 2,524,603 + +0.4 2,524,603 + -2.2 2,524,603 + -0.8 2,524,603 + -0.8 2,524,603 + +0.8 2,524,603 + -2.2 2,524,603 + -1.7 2,524,603 + +0.1 2,524,603 + -1.8 2,524,604 - -1.6 2,524,604 - -0.7 2,524,604 - -2.5 2,524,604 - +1.4 2,524,604 - -1.7 2,524,604 - -0.6 2,524,604 - -1.0 2,524,604 - +1.8 2,524,604 - +2.4 2,524,604 - -1.0 2,524,604 - -2.0 2,524,604 - -1.6 2,524,604 - -0.5 2,524,604 - -2.1 2,524,604 - -2.5 2,524,604 - -2.3 2,524,604 - +0.4 2,524,604 - +1.4 2,524,604 - -2.9 2,524,604 - +0.6 2,524,604 - -0.4 2,524,604 - -0.2 2,524,630 + LRK54_RS11395 0.11 -1.1 2,524,645 + LRK54_RS11395 0.13 -0.6 2,524,646 - LRK54_RS11395 0.14 -2.2 2,524,855 + LRK54_RS11395 0.44 -1.7 2,524,855 + LRK54_RS11395 0.44 -2.3 2,524,855 + LRK54_RS11395 0.44 +1.2 2,524,855 + LRK54_RS11395 0.44 -1.2 2,524,855 + LRK54_RS11395 0.44 -1.9 2,524,855 + LRK54_RS11395 0.44 -0.8 2,524,855 + LRK54_RS11395 0.44 -0.2 2,524,855 + LRK54_RS11395 0.44 -0.6 2,524,855 + LRK54_RS11395 0.44 +0.6 2,524,855 + LRK54_RS11395 0.44 -1.7 2,524,855 + LRK54_RS11395 0.44 +1.4 2,524,856 - LRK54_RS11395 0.44 -0.5 2,524,856 - LRK54_RS11395 0.44 -0.7 2,524,856 - LRK54_RS11395 0.44 -0.9 2,524,856 - LRK54_RS11395 0.44 -1.2 2,524,856 - LRK54_RS11395 0.44 -3.1 2,524,856 - LRK54_RS11395 0.44 -2.4 2,524,856 - LRK54_RS11395 0.44 -0.3 2,524,856 - LRK54_RS11395 0.44 -2.1 2,525,406 + LRK54_RS11400 0.29 +0.6 2,525,406 + LRK54_RS11400 0.29 -0.6 2,525,406 + LRK54_RS11400 0.29 +2.4 2,525,406 + LRK54_RS11400 0.29 -0.3 2,525,406 + LRK54_RS11400 0.29 -0.5 2,525,406 + LRK54_RS11400 0.29 +0.1 2,525,406 + LRK54_RS11400 0.29 -0.6 2,525,406 + LRK54_RS11400 0.29 -1.1 2,525,406 + LRK54_RS11400 0.29 +0.8 2,525,406 + LRK54_RS11400 0.29 -0.5 2,525,406 + LRK54_RS11400 0.29 -1.5 2,525,406 + LRK54_RS11400 0.29 -0.6 2,525,406 + LRK54_RS11400 0.29 -0.5 2,525,406 + LRK54_RS11400 0.29 -0.1 2,525,406 + LRK54_RS11400 0.29 +0.0 2,525,406 + LRK54_RS11400 0.29 -1.6 2,525,406 + LRK54_RS11400 0.29 +0.9 2,525,406 + LRK54_RS11400 0.29 -1.0 2,525,406 + LRK54_RS11400 0.29 -0.6 2,525,406 + LRK54_RS11400 0.29 +0.2 2,525,406 + LRK54_RS11400 0.29 +0.6 2,525,406 + LRK54_RS11400 0.29 -0.3 2,525,406 + LRK54_RS11400 0.29 -0.9 2,525,406 + LRK54_RS11400 0.29 +1.0 2,525,406 + LRK54_RS11400 0.29 +0.7 2,525,406 + LRK54_RS11400 0.29 -1.0 2,525,406 + LRK54_RS11400 0.29 -0.6 2,525,407 - LRK54_RS11400 0.29 -1.5 2,525,407 - LRK54_RS11400 0.29 +0.6 2,525,407 - LRK54_RS11400 0.29 +0.4 2,525,407 - LRK54_RS11400 0.29 +0.9 2,525,407 - LRK54_RS11400 0.29 -1.4 2,525,407 - LRK54_RS11400 0.29 -0.2 2,525,407 - LRK54_RS11400 0.29 -0.5 2,525,407 - LRK54_RS11400 0.29 +0.4 2,525,407 - LRK54_RS11400 0.29 -0.1 2,525,407 - LRK54_RS11400 0.29 -0.3 2,525,407 - LRK54_RS11400 0.29 -0.8 2,525,407 - LRK54_RS11400 0.29 +0.2 2,525,407 - LRK54_RS11400 0.29 -3.0 2,525,407 - LRK54_RS11400 0.29 +0.9 2,525,407 - LRK54_RS11400 0.29 +0.7 2,525,407 - LRK54_RS11400 0.29 +0.3 2,525,407 - LRK54_RS11400 0.29 +1.3 2,525,407 - LRK54_RS11400 0.29 +0.1 2,525,407 - LRK54_RS11400 0.29 -2.3 2,525,407 - LRK54_RS11400 0.29 -1.0 2,525,407 - LRK54_RS11400 0.29 +0.4 2,525,407 - LRK54_RS11400 0.29 +0.4 2,525,407 - LRK54_RS11400 0.29 -1.3 2,525,407 - LRK54_RS11400 0.29 +0.1 2,525,407 - LRK54_RS11400 0.29 +0.1 2,525,439 + LRK54_RS11400 0.35 +1.0 2,525,439 + LRK54_RS11400 0.35 -0.1 2,525,439 + LRK54_RS11400 0.35 -0.7 2,525,439 + LRK54_RS11400 0.35 +0.7 2,525,439 + LRK54_RS11400 0.35 -2.2 2,525,439 + LRK54_RS11400 0.35 -1.7 2,525,439 + LRK54_RS11400 0.35 -0.8 2,525,440 - LRK54_RS11400 0.35 -0.7 2,525,440 - LRK54_RS11400 0.35 +0.5 2,525,440 - LRK54_RS11400 0.35 -0.2 2,525,544 + LRK54_RS11400 0.53 -0.6 2,525,544 + LRK54_RS11400 0.53 -0.7 2,525,573 - LRK54_RS11400 0.58 -0.9 2,525,622 + LRK54_RS11400 0.66 +0.6 2,525,622 + LRK54_RS11400 0.66 -0.7 2,525,623 - LRK54_RS11400 0.66 -0.2 2,525,623 - LRK54_RS11400 0.66 +2.0 2,525,623 - LRK54_RS11400 0.66 -1.9 2,525,623 - LRK54_RS11400 0.66 -0.1 2,525,623 - LRK54_RS11400 0.66 -0.4 2,525,623 - LRK54_RS11400 0.66 -1.7 2,525,623 - LRK54_RS11400 0.66 +0.4 2,525,623 - LRK54_RS11400 0.66 -1.3 2,525,679 - LRK54_RS11400 0.76 -0.9 2,525,679 - LRK54_RS11400 0.76 -0.4 2,525,901 + -1.5 2,525,901 + +0.8 2,525,901 + +0.9 2,525,901 + -2.0 2,525,901 + -2.0 2,525,901 + -1.4 2,525,902 - -0.7 2,525,902 - +0.4 2,525,902 - +0.3 2,525,902 - +0.6 2,525,902 - -0.3 2,525,902 - -1.0 2,525,902 - +0.0 2,525,902 - -0.1 2,525,902 - -0.7 2,525,902 - -1.4 2,525,902 - -1.0 2,525,902 - +0.7 2,525,902 - -1.5 2,525,902 - -0.6 2,525,902 - +2.4 2,525,902 - +0.1 2,526,027 + +0.0 2,526,027 + -0.2 2,526,027 + -1.0 2,526,027 + -0.9 2,526,027 + -1.1 2,526,027 + -0.5 2,526,027 + +0.7 2,526,027 + -1.1 2,526,027 + -1.6 2,526,028 - -0.7 2,526,028 - -0.1 2,526,028 - +0.1 2,526,028 - -1.0 2,526,028 - -2.1 2,526,028 - +1.9 2,526,028 - +0.4 2,526,028 - -0.3 2,526,028 - -0.6 2,526,028 - -2.0 2,526,028 - -2.1 2,526,067 - LRK54_RS11405 0.12 -1.4 2,526,162 + LRK54_RS11405 0.16 +0.4 2,526,162 + LRK54_RS11405 0.16 -0.8 2,526,162 + LRK54_RS11405 0.16 -1.9 2,526,162 + LRK54_RS11405 0.16 -1.4 2,526,163 - LRK54_RS11405 0.16 -0.7 2,526,163 - LRK54_RS11405 0.16 -0.7
Or see this region's nucleotide sequence