Experiment: R2A with D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21905 and CA265_RS21910 are separated by 206 nucleotides CA265_RS21910 and CA265_RS21915 are separated by 6 nucleotides
CA265_RS21905: CA265_RS21905 - hypothetical protein, at 5,382,188 to 5,383,090
_RS21905
CA265_RS21910: CA265_RS21910 - glycoside hydrolase, at 5,383,297 to 5,384,841
_RS21910
CA265_RS21915: CA265_RS21915 - LacI family transcriptional regulator, at 5,384,848 to 5,385,870
_RS21915
Position (kb)
5383
5384
5385 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5382.329 kb on - strand, within CA265_RS21905 at 5382.347 kb on - strand, within CA265_RS21905 at 5382.421 kb on - strand, within CA265_RS21905 at 5382.569 kb on + strand, within CA265_RS21905 at 5382.620 kb on + strand, within CA265_RS21905 at 5382.635 kb on - strand, within CA265_RS21905 at 5382.691 kb on + strand, within CA265_RS21905 at 5382.817 kb on + strand, within CA265_RS21905 at 5382.955 kb on + strand, within CA265_RS21905 at 5382.955 kb on + strand, within CA265_RS21905 at 5383.002 kb on + strand at 5383.002 kb on + strand at 5383.020 kb on - strand at 5383.061 kb on - strand at 5383.070 kb on - strand at 5383.094 kb on + strand at 5383.119 kb on + strand at 5383.122 kb on + strand at 5383.140 kb on - strand at 5383.144 kb on - strand at 5383.168 kb on - strand at 5383.233 kb on + strand at 5383.296 kb on - strand at 5383.338 kb on - strand at 5383.340 kb on + strand at 5383.346 kb on - strand at 5383.346 kb on - strand at 5383.346 kb on - strand at 5383.348 kb on - strand at 5383.348 kb on - strand at 5383.395 kb on + strand at 5383.395 kb on + strand at 5383.419 kb on + strand at 5383.501 kb on - strand, within CA265_RS21910 at 5383.501 kb on - strand, within CA265_RS21910 at 5383.524 kb on + strand, within CA265_RS21910 at 5383.525 kb on - strand, within CA265_RS21910 at 5383.610 kb on + strand, within CA265_RS21910 at 5383.711 kb on - strand, within CA265_RS21910 at 5383.818 kb on - strand, within CA265_RS21910 at 5383.826 kb on - strand, within CA265_RS21910 at 5383.826 kb on - strand, within CA265_RS21910 at 5383.827 kb on + strand, within CA265_RS21910 at 5383.828 kb on - strand, within CA265_RS21910 at 5383.862 kb on - strand, within CA265_RS21910 at 5383.862 kb on - strand, within CA265_RS21910 at 5383.904 kb on - strand, within CA265_RS21910 at 5383.905 kb on + strand, within CA265_RS21910 at 5383.910 kb on + strand, within CA265_RS21910 at 5383.951 kb on + strand, within CA265_RS21910 at 5383.951 kb on + strand, within CA265_RS21910 at 5384.025 kb on + strand, within CA265_RS21910 at 5384.026 kb on - strand, within CA265_RS21910 at 5384.029 kb on - strand, within CA265_RS21910 at 5384.092 kb on + strand, within CA265_RS21910 at 5384.092 kb on + strand, within CA265_RS21910 at 5384.135 kb on - strand, within CA265_RS21910 at 5384.160 kb on + strand, within CA265_RS21910 at 5384.161 kb on - strand, within CA265_RS21910 at 5384.184 kb on - strand, within CA265_RS21910 at 5384.188 kb on + strand, within CA265_RS21910 at 5384.265 kb on + strand, within CA265_RS21910 at 5384.265 kb on + strand, within CA265_RS21910 at 5384.287 kb on + strand, within CA265_RS21910 at 5384.288 kb on - strand, within CA265_RS21910 at 5384.289 kb on + strand, within CA265_RS21910 at 5384.290 kb on - strand, within CA265_RS21910 at 5384.305 kb on + strand, within CA265_RS21910 at 5384.305 kb on + strand, within CA265_RS21910 at 5384.306 kb on - strand, within CA265_RS21910 at 5384.371 kb on + strand, within CA265_RS21910 at 5384.371 kb on + strand, within CA265_RS21910 at 5384.430 kb on + strand, within CA265_RS21910 at 5384.430 kb on + strand, within CA265_RS21910 at 5384.476 kb on - strand, within CA265_RS21910 at 5384.479 kb on + strand, within CA265_RS21910 at 5384.479 kb on + strand, within CA265_RS21910 at 5384.479 kb on + strand, within CA265_RS21910 at 5384.570 kb on + strand, within CA265_RS21910 at 5384.571 kb on - strand, within CA265_RS21910 at 5384.571 kb on - strand, within CA265_RS21910 at 5384.635 kb on + strand, within CA265_RS21910 at 5384.689 kb on - strand at 5384.694 kb on - strand at 5384.733 kb on - strand at 5384.751 kb on + strand at 5384.752 kb on - strand at 5384.840 kb on - strand at 5384.850 kb on - strand at 5384.904 kb on + strand at 5384.905 kb on - strand at 5384.905 kb on - strand at 5384.905 kb on - strand at 5384.958 kb on + strand, within CA265_RS21915 at 5385.009 kb on + strand, within CA265_RS21915 at 5385.010 kb on - strand, within CA265_RS21915 at 5385.010 kb on - strand, within CA265_RS21915 at 5385.016 kb on - strand, within CA265_RS21915 at 5385.087 kb on + strand, within CA265_RS21915 at 5385.133 kb on - strand, within CA265_RS21915 at 5385.136 kb on - strand, within CA265_RS21915 at 5385.213 kb on + strand, within CA265_RS21915 at 5385.214 kb on - strand, within CA265_RS21915 at 5385.217 kb on - strand, within CA265_RS21915 at 5385.227 kb on - strand, within CA265_RS21915 at 5385.230 kb on - strand, within CA265_RS21915 at 5385.271 kb on + strand, within CA265_RS21915 at 5385.283 kb on - strand, within CA265_RS21915 at 5385.286 kb on + strand, within CA265_RS21915 at 5385.291 kb on + strand, within CA265_RS21915 at 5385.361 kb on - strand, within CA265_RS21915 at 5385.409 kb on + strand, within CA265_RS21915 at 5385.430 kb on + strand, within CA265_RS21915 at 5385.431 kb on - strand, within CA265_RS21915 at 5385.431 kb on - strand, within CA265_RS21915 at 5385.431 kb on - strand, within CA265_RS21915 at 5385.432 kb on + strand, within CA265_RS21915 at 5385.432 kb on + strand, within CA265_RS21915 at 5385.454 kb on - strand, within CA265_RS21915 at 5385.515 kb on - strand, within CA265_RS21915 at 5385.538 kb on + strand, within CA265_RS21915 at 5385.539 kb on - strand, within CA265_RS21915 at 5385.547 kb on + strand, within CA265_RS21915 at 5385.548 kb on - strand, within CA265_RS21915 at 5385.579 kb on - strand, within CA265_RS21915 at 5385.582 kb on + strand, within CA265_RS21915 at 5385.622 kb on - strand, within CA265_RS21915 at 5385.633 kb on + strand, within CA265_RS21915 at 5385.634 kb on - strand, within CA265_RS21915 at 5385.634 kb on - strand, within CA265_RS21915 at 5385.634 kb on - strand, within CA265_RS21915 at 5385.655 kb on + strand, within CA265_RS21915 at 5385.656 kb on - strand, within CA265_RS21915 at 5385.671 kb on - strand, within CA265_RS21915 at 5385.671 kb on - strand, within CA265_RS21915 at 5385.671 kb on - strand, within CA265_RS21915 at 5385.718 kb on - strand, within CA265_RS21915 at 5385.795 kb on + strand at 5385.818 kb on - strand at 5385.826 kb on - strand at 5385.841 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.12 mg/ml remove 5,382,329 - CA265_RS21905 0.16 +1.3 5,382,347 - CA265_RS21905 0.18 +0.2 5,382,421 - CA265_RS21905 0.26 +0.3 5,382,569 + CA265_RS21905 0.42 -0.4 5,382,620 + CA265_RS21905 0.48 +0.1 5,382,635 - CA265_RS21905 0.50 +0.4 5,382,691 + CA265_RS21905 0.56 +0.4 5,382,817 + CA265_RS21905 0.70 -0.2 5,382,955 + CA265_RS21905 0.85 +1.3 5,382,955 + CA265_RS21905 0.85 -0.7 5,383,002 + -0.3 5,383,002 + +1.7 5,383,020 - -0.0 5,383,061 - +0.3 5,383,070 - +0.3 5,383,094 + +0.4 5,383,119 + -1.2 5,383,122 + +0.7 5,383,140 - -0.0 5,383,144 - +0.6 5,383,168 - -1.0 5,383,233 + +0.4 5,383,296 - -0.1 5,383,338 - +0.4 5,383,340 + -0.1 5,383,346 - +0.2 5,383,346 - -0.2 5,383,346 - +0.1 5,383,348 - +0.4 5,383,348 - -0.2 5,383,395 + +0.9 5,383,395 + -1.0 5,383,419 + +0.7 5,383,501 - CA265_RS21910 0.13 +0.6 5,383,501 - CA265_RS21910 0.13 +0.2 5,383,524 + CA265_RS21910 0.15 +0.5 5,383,525 - CA265_RS21910 0.15 -0.3 5,383,610 + CA265_RS21910 0.20 +0.5 5,383,711 - CA265_RS21910 0.27 +0.3 5,383,818 - CA265_RS21910 0.34 +0.5 5,383,826 - CA265_RS21910 0.34 +0.5 5,383,826 - CA265_RS21910 0.34 +2.2 5,383,827 + CA265_RS21910 0.34 +0.3 5,383,828 - CA265_RS21910 0.34 -0.1 5,383,862 - CA265_RS21910 0.37 +1.0 5,383,862 - CA265_RS21910 0.37 +1.2 5,383,904 - CA265_RS21910 0.39 +1.2 5,383,905 + CA265_RS21910 0.39 +0.1 5,383,910 + CA265_RS21910 0.40 -0.1 5,383,951 + CA265_RS21910 0.42 -0.7 5,383,951 + CA265_RS21910 0.42 +0.2 5,384,025 + CA265_RS21910 0.47 -0.2 5,384,026 - CA265_RS21910 0.47 +0.5 5,384,029 - CA265_RS21910 0.47 +0.6 5,384,092 + CA265_RS21910 0.51 +1.5 5,384,092 + CA265_RS21910 0.51 +1.3 5,384,135 - CA265_RS21910 0.54 -0.0 5,384,160 + CA265_RS21910 0.56 +2.0 5,384,161 - CA265_RS21910 0.56 +0.6 5,384,184 - CA265_RS21910 0.57 +0.6 5,384,188 + CA265_RS21910 0.58 +0.7 5,384,265 + CA265_RS21910 0.63 +0.2 5,384,265 + CA265_RS21910 0.63 -0.3 5,384,287 + CA265_RS21910 0.64 +0.1 5,384,288 - CA265_RS21910 0.64 +0.3 5,384,289 + CA265_RS21910 0.64 +1.0 5,384,290 - CA265_RS21910 0.64 +0.3 5,384,305 + CA265_RS21910 0.65 +1.0 5,384,305 + CA265_RS21910 0.65 +1.1 5,384,306 - CA265_RS21910 0.65 +0.7 5,384,371 + CA265_RS21910 0.70 +0.7 5,384,371 + CA265_RS21910 0.70 -0.9 5,384,430 + CA265_RS21910 0.73 +0.4 5,384,430 + CA265_RS21910 0.73 +0.7 5,384,476 - CA265_RS21910 0.76 +0.7 5,384,479 + CA265_RS21910 0.77 +0.9 5,384,479 + CA265_RS21910 0.77 -0.6 5,384,479 + CA265_RS21910 0.77 +0.1 5,384,570 + CA265_RS21910 0.82 -0.1 5,384,571 - CA265_RS21910 0.82 +0.7 5,384,571 - CA265_RS21910 0.82 +2.3 5,384,635 + CA265_RS21910 0.87 -0.1 5,384,689 - -0.0 5,384,694 - +0.7 5,384,733 - +1.1 5,384,751 + +0.3 5,384,752 - +0.2 5,384,840 - +0.5 5,384,850 - +0.3 5,384,904 + -0.0 5,384,905 - -0.3 5,384,905 - +0.4 5,384,905 - +1.1 5,384,958 + CA265_RS21915 0.11 +2.2 5,385,009 + CA265_RS21915 0.16 -0.2 5,385,010 - CA265_RS21915 0.16 +0.4 5,385,010 - CA265_RS21915 0.16 +0.9 5,385,016 - CA265_RS21915 0.16 +0.3 5,385,087 + CA265_RS21915 0.23 +0.0 5,385,133 - CA265_RS21915 0.28 -0.2 5,385,136 - CA265_RS21915 0.28 +0.7 5,385,213 + CA265_RS21915 0.36 -0.1 5,385,214 - CA265_RS21915 0.36 -0.1 5,385,217 - CA265_RS21915 0.36 -0.4 5,385,227 - CA265_RS21915 0.37 +0.3 5,385,230 - CA265_RS21915 0.37 +0.6 5,385,271 + CA265_RS21915 0.41 -0.5 5,385,283 - CA265_RS21915 0.43 +0.8 5,385,286 + CA265_RS21915 0.43 +0.2 5,385,291 + CA265_RS21915 0.43 +0.8 5,385,361 - CA265_RS21915 0.50 +0.0 5,385,409 + CA265_RS21915 0.55 +0.9 5,385,430 + CA265_RS21915 0.57 +0.2 5,385,431 - CA265_RS21915 0.57 -0.0 5,385,431 - CA265_RS21915 0.57 +0.7 5,385,431 - CA265_RS21915 0.57 +0.1 5,385,432 + CA265_RS21915 0.57 +0.6 5,385,432 + CA265_RS21915 0.57 +0.4 5,385,454 - CA265_RS21915 0.59 +0.5 5,385,515 - CA265_RS21915 0.65 -0.6 5,385,538 + CA265_RS21915 0.67 +0.4 5,385,539 - CA265_RS21915 0.68 +0.6 5,385,547 + CA265_RS21915 0.68 -0.3 5,385,548 - CA265_RS21915 0.68 -0.2 5,385,579 - CA265_RS21915 0.71 +1.8 5,385,582 + CA265_RS21915 0.72 +0.1 5,385,622 - CA265_RS21915 0.76 +0.3 5,385,633 + CA265_RS21915 0.77 +1.8 5,385,634 - CA265_RS21915 0.77 +0.0 5,385,634 - CA265_RS21915 0.77 -0.6 5,385,634 - CA265_RS21915 0.77 +0.3 5,385,655 + CA265_RS21915 0.79 -0.0 5,385,656 - CA265_RS21915 0.79 -0.5 5,385,671 - CA265_RS21915 0.80 +0.1 5,385,671 - CA265_RS21915 0.80 -0.0 5,385,671 - CA265_RS21915 0.80 +0.4 5,385,718 - CA265_RS21915 0.85 -3.5 5,385,795 + -1.3 5,385,818 - +1.0 5,385,826 - -0.1 5,385,841 + +1.6
Or see this region's nucleotide sequence