Experiment: R2A with D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15810 and CA265_RS15815 are separated by 97 nucleotides CA265_RS15815 and CA265_RS15820 are separated by 18 nucleotides CA265_RS15820 and CA265_RS15825 are separated by 126 nucleotides
CA265_RS15810: CA265_RS15810 - acetohydroxybutanoate synthase regulatory subunit (EC 2.2.1.6) (from data) , at 3,980,104 to 3,980,742
_RS15810
CA265_RS15815: CA265_RS15815 - glyoxalase/bleomycin resistance/extradiol dioxygenase family protein, at 3,980,840 to 3,981,229
_RS15815
CA265_RS15820: CA265_RS15820 - hypothetical protein, at 3,981,248 to 3,981,712
_RS15820
CA265_RS15825: CA265_RS15825 - ketol-acid reductoisomerase, at 3,981,839 to 3,983,320
_RS15825
Position (kb)
3981
3982 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3980.259 kb on - strand, within CA265_RS15810 at 3980.259 kb on - strand, within CA265_RS15810 at 3980.289 kb on - strand, within CA265_RS15810 at 3980.381 kb on - strand, within CA265_RS15810 at 3980.486 kb on - strand, within CA265_RS15810 at 3980.519 kb on - strand, within CA265_RS15810 at 3980.585 kb on - strand, within CA265_RS15810 at 3980.737 kb on - strand at 3980.761 kb on + strand at 3980.839 kb on + strand at 3980.869 kb on - strand at 3980.923 kb on + strand, within CA265_RS15815 at 3980.924 kb on - strand, within CA265_RS15815 at 3980.928 kb on + strand, within CA265_RS15815 at 3980.998 kb on - strand, within CA265_RS15815 at 3981.045 kb on - strand, within CA265_RS15815 at 3981.045 kb on - strand, within CA265_RS15815 at 3981.084 kb on + strand, within CA265_RS15815 at 3981.182 kb on - strand, within CA265_RS15815 at 3981.182 kb on - strand, within CA265_RS15815 at 3981.236 kb on - strand at 3981.236 kb on - strand at 3981.243 kb on - strand at 3981.244 kb on + strand at 3981.307 kb on - strand, within CA265_RS15820 at 3981.307 kb on - strand, within CA265_RS15820 at 3981.351 kb on - strand, within CA265_RS15820 at 3981.353 kb on - strand, within CA265_RS15820 at 3981.394 kb on + strand, within CA265_RS15820 at 3981.564 kb on - strand, within CA265_RS15820 at 3981.612 kb on - strand, within CA265_RS15820 at 3981.612 kb on - strand, within CA265_RS15820 at 3981.634 kb on - strand, within CA265_RS15820 at 3981.634 kb on - strand, within CA265_RS15820 at 3981.673 kb on - strand at 3981.678 kb on - strand at 3981.678 kb on - strand at 3981.678 kb on - strand at 3981.711 kb on - strand at 3981.757 kb on - strand at 3981.813 kb on - strand at 3981.879 kb on + strand at 3981.943 kb on - strand at 3981.943 kb on - strand at 3981.943 kb on - strand at 3981.967 kb on - strand at 3982.013 kb on - strand, within CA265_RS15825 at 3982.026 kb on + strand, within CA265_RS15825 at 3982.027 kb on - strand, within CA265_RS15825 at 3982.043 kb on - strand, within CA265_RS15825 at 3982.099 kb on + strand, within CA265_RS15825 at 3982.140 kb on + strand, within CA265_RS15825 at 3982.141 kb on - strand, within CA265_RS15825 at 3982.143 kb on + strand, within CA265_RS15825 at 3982.153 kb on + strand, within CA265_RS15825 at 3982.154 kb on - strand, within CA265_RS15825 at 3982.249 kb on - strand, within CA265_RS15825 at 3982.289 kb on - strand, within CA265_RS15825 at 3982.301 kb on - strand, within CA265_RS15825 at 3982.332 kb on - strand, within CA265_RS15825 at 3982.419 kb on - strand, within CA265_RS15825 at 3982.430 kb on - strand, within CA265_RS15825 at 3982.525 kb on - strand, within CA265_RS15825 at 3982.542 kb on + strand, within CA265_RS15825 at 3982.553 kb on - strand, within CA265_RS15825 at 3982.561 kb on - strand, within CA265_RS15825 at 3982.605 kb on - strand, within CA265_RS15825 at 3982.605 kb on - strand, within CA265_RS15825 at 3982.617 kb on + strand, within CA265_RS15825 at 3982.646 kb on - strand, within CA265_RS15825
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.12 mg/ml remove 3,980,259 - CA265_RS15810 0.24 +1.1 3,980,259 - CA265_RS15810 0.24 -0.2 3,980,289 - CA265_RS15810 0.29 +0.2 3,980,381 - CA265_RS15810 0.43 +1.1 3,980,486 - CA265_RS15810 0.60 +0.6 3,980,519 - CA265_RS15810 0.65 +0.9 3,980,585 - CA265_RS15810 0.75 +0.3 3,980,737 - +0.3 3,980,761 + +0.9 3,980,839 + -1.2 3,980,869 - -1.0 3,980,923 + CA265_RS15815 0.21 -0.9 3,980,924 - CA265_RS15815 0.22 -0.2 3,980,928 + CA265_RS15815 0.23 +1.0 3,980,998 - CA265_RS15815 0.41 +2.2 3,981,045 - CA265_RS15815 0.53 -0.8 3,981,045 - CA265_RS15815 0.53 -0.0 3,981,084 + CA265_RS15815 0.63 +0.6 3,981,182 - CA265_RS15815 0.88 +0.4 3,981,182 - CA265_RS15815 0.88 +1.1 3,981,236 - -0.1 3,981,236 - -1.0 3,981,243 - +0.4 3,981,244 + +0.3 3,981,307 - CA265_RS15820 0.13 -0.0 3,981,307 - CA265_RS15820 0.13 +0.2 3,981,351 - CA265_RS15820 0.22 -0.1 3,981,353 - CA265_RS15820 0.23 +0.1 3,981,394 + CA265_RS15820 0.31 +2.7 3,981,564 - CA265_RS15820 0.68 +0.7 3,981,612 - CA265_RS15820 0.78 -0.2 3,981,612 - CA265_RS15820 0.78 +1.6 3,981,634 - CA265_RS15820 0.83 +0.0 3,981,634 - CA265_RS15820 0.83 +0.6 3,981,673 - +0.6 3,981,678 - -0.5 3,981,678 - -0.5 3,981,678 - -0.4 3,981,711 - +1.3 3,981,757 - +0.5 3,981,813 - +0.6 3,981,879 + -0.2 3,981,943 - +0.3 3,981,943 - -0.0 3,981,943 - +0.3 3,981,967 - -0.2 3,982,013 - CA265_RS15825 0.12 -0.4 3,982,026 + CA265_RS15825 0.13 +1.1 3,982,027 - CA265_RS15825 0.13 +0.2 3,982,043 - CA265_RS15825 0.14 -0.6 3,982,099 + CA265_RS15825 0.18 +0.3 3,982,140 + CA265_RS15825 0.20 -0.4 3,982,141 - CA265_RS15825 0.20 -0.8 3,982,143 + CA265_RS15825 0.21 +1.3 3,982,153 + CA265_RS15825 0.21 -0.4 3,982,154 - CA265_RS15825 0.21 -0.2 3,982,249 - CA265_RS15825 0.28 +0.7 3,982,289 - CA265_RS15825 0.30 -0.7 3,982,301 - CA265_RS15825 0.31 -1.0 3,982,332 - CA265_RS15825 0.33 -0.0 3,982,419 - CA265_RS15825 0.39 -0.3 3,982,430 - CA265_RS15825 0.40 +0.2 3,982,525 - CA265_RS15825 0.46 +1.0 3,982,542 + CA265_RS15825 0.47 -2.2 3,982,553 - CA265_RS15825 0.48 -0.3 3,982,561 - CA265_RS15825 0.49 +0.2 3,982,605 - CA265_RS15825 0.52 +0.3 3,982,605 - CA265_RS15825 0.52 +0.5 3,982,617 + CA265_RS15825 0.52 +1.0 3,982,646 - CA265_RS15825 0.54 +0.1
Or see this region's nucleotide sequence