Experiment: R2A with D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10865 and CA265_RS10870 are separated by 184 nucleotides CA265_RS10870 and CA265_RS10875 are separated by 138 nucleotides CA265_RS10875 and CA265_RS10880 are separated by 29 nucleotides CA265_RS10880 and CA265_RS10885 are separated by 115 nucleotides
CA265_RS10865: CA265_RS10865 - general stress protein, at 2,678,269 to 2,678,823
_RS10865
CA265_RS10870: CA265_RS10870 - hypothetical protein, at 2,679,008 to 2,679,505
_RS10870
CA265_RS10875: CA265_RS10875 - hypothetical protein, at 2,679,644 to 2,679,847
_RS10875
CA265_RS10880: CA265_RS10880 - DUF2892 domain-containing protein, at 2,679,877 to 2,680,416
_RS10880
CA265_RS10885: CA265_RS10885 - thiol reductase thioredoxin, at 2,680,532 to 2,681,050
_RS10885
Position (kb)
2679
2680 Strain fitness (log2 ratio)
-1
0
1 at 2678.686 kb on - strand, within CA265_RS10865 at 2678.725 kb on + strand, within CA265_RS10865 at 2678.793 kb on + strand at 2678.948 kb on - strand at 2678.948 kb on - strand at 2678.948 kb on - strand at 2678.958 kb on + strand at 2678.969 kb on - strand at 2678.997 kb on - strand at 2679.143 kb on - strand at 2679.218 kb on - strand, within CA265_RS10870 at 2679.284 kb on - strand, within CA265_RS10870 at 2679.340 kb on + strand, within CA265_RS10870 at 2679.394 kb on + strand, within CA265_RS10870 at 2679.396 kb on + strand, within CA265_RS10870 at 2679.396 kb on + strand, within CA265_RS10870 at 2679.397 kb on - strand, within CA265_RS10870 at 2679.503 kb on + strand at 2679.562 kb on - strand at 2679.564 kb on - strand at 2679.586 kb on + strand at 2679.673 kb on + strand, within CA265_RS10875 at 2679.691 kb on + strand, within CA265_RS10875 at 2679.703 kb on - strand, within CA265_RS10875 at 2679.705 kb on + strand, within CA265_RS10875 at 2679.706 kb on - strand, within CA265_RS10875 at 2679.774 kb on + strand, within CA265_RS10875 at 2679.859 kb on - strand at 2679.879 kb on - strand at 2680.527 kb on + strand at 2680.550 kb on - strand at 2680.633 kb on - strand, within CA265_RS10885 at 2680.635 kb on + strand, within CA265_RS10885 at 2680.648 kb on + strand, within CA265_RS10885 at 2680.712 kb on + strand, within CA265_RS10885 at 2680.716 kb on + strand, within CA265_RS10885 at 2680.716 kb on + strand, within CA265_RS10885 at 2680.804 kb on + strand, within CA265_RS10885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.12 mg/ml remove 2,678,686 - CA265_RS10865 0.75 -0.4 2,678,725 + CA265_RS10865 0.82 +0.7 2,678,793 + -0.4 2,678,948 - -0.8 2,678,948 - +0.6 2,678,948 - -0.0 2,678,958 + +0.0 2,678,969 - +0.4 2,678,997 - +0.7 2,679,143 - -0.8 2,679,218 - CA265_RS10870 0.42 -1.1 2,679,284 - CA265_RS10870 0.55 +0.7 2,679,340 + CA265_RS10870 0.67 +0.0 2,679,394 + CA265_RS10870 0.78 -1.5 2,679,396 + CA265_RS10870 0.78 -0.0 2,679,396 + CA265_RS10870 0.78 -0.1 2,679,397 - CA265_RS10870 0.78 +0.2 2,679,503 + -0.3 2,679,562 - -0.4 2,679,564 - -1.2 2,679,586 + -0.6 2,679,673 + CA265_RS10875 0.14 +0.4 2,679,691 + CA265_RS10875 0.23 +0.2 2,679,703 - CA265_RS10875 0.29 +0.5 2,679,705 + CA265_RS10875 0.30 +0.0 2,679,706 - CA265_RS10875 0.30 +0.5 2,679,774 + CA265_RS10875 0.64 +0.5 2,679,859 - -0.1 2,679,879 - -0.3 2,680,527 + +1.2 2,680,550 - +0.6 2,680,633 - CA265_RS10885 0.19 -1.7 2,680,635 + CA265_RS10885 0.20 +0.3 2,680,648 + CA265_RS10885 0.22 -0.4 2,680,712 + CA265_RS10885 0.35 +0.9 2,680,716 + CA265_RS10885 0.35 +0.9 2,680,716 + CA265_RS10885 0.35 +0.6 2,680,804 + CA265_RS10885 0.52 +1.3
Or see this region's nucleotide sequence