Strain Fitness in Pedobacter sp. GW460-11-11-14-LB5 around CA265_RS08945

Experiment: R2A with D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCA265_RS08930 and CA265_RS08935 are separated by 107 nucleotidesCA265_RS08935 and CA265_RS08940 are separated by 61 nucleotidesCA265_RS08940 and CA265_RS08945 are separated by 3 nucleotidesCA265_RS08945 and CA265_RS08950 are separated by 153 nucleotides CA265_RS08930: CA265_RS08930 - 7-carboxy-7-deazaguanine synthase QueE, at 2,230,973 to 2,231,596 _RS08930 CA265_RS08935: CA265_RS08935 - (2Fe-2S)-binding protein, at 2,231,704 to 2,232,039 _RS08935 CA265_RS08940: CA265_RS08940 - twitching motility protein PilT, at 2,232,101 to 2,232,520 _RS08940 CA265_RS08945: CA265_RS08945 - hypothetical protein, at 2,232,524 to 2,232,784 _RS08945 CA265_RS08950: CA265_RS08950 - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase, at 2,232,938 to 2,233,858 _RS08950 Position (kb) 2232 2233Strain fitness (log2 ratio) -2 -1 0 1at 2231.534 kb on + strandat 2231.539 kb on + strandat 2231.565 kb on - strandat 2231.565 kb on - strandat 2231.583 kb on - strandat 2231.684 kb on - strandat 2231.692 kb on + strandat 2231.723 kb on + strandat 2231.728 kb on - strandat 2231.728 kb on - strandat 2231.809 kb on - strand, within CA265_RS08935at 2231.913 kb on + strand, within CA265_RS08935at 2231.913 kb on + strand, within CA265_RS08935at 2231.913 kb on + strand, within CA265_RS08935at 2231.953 kb on + strand, within CA265_RS08935at 2231.953 kb on + strand, within CA265_RS08935at 2231.954 kb on - strand, within CA265_RS08935at 2231.995 kb on + strand, within CA265_RS08935at 2231.996 kb on - strand, within CA265_RS08935at 2231.996 kb on - strand, within CA265_RS08935at 2232.173 kb on + strand, within CA265_RS08940at 2232.174 kb on - strand, within CA265_RS08940at 2232.175 kb on + strand, within CA265_RS08940at 2232.196 kb on + strand, within CA265_RS08940at 2232.196 kb on + strand, within CA265_RS08940at 2232.196 kb on + strand, within CA265_RS08940at 2232.196 kb on + strand, within CA265_RS08940at 2232.197 kb on - strand, within CA265_RS08940at 2232.197 kb on - strand, within CA265_RS08940at 2232.197 kb on - strand, within CA265_RS08940at 2232.197 kb on - strand, within CA265_RS08940at 2232.199 kb on + strand, within CA265_RS08940at 2232.199 kb on + strand, within CA265_RS08940at 2232.220 kb on - strand, within CA265_RS08940at 2232.222 kb on + strand, within CA265_RS08940at 2232.304 kb on + strand, within CA265_RS08940at 2232.305 kb on - strand, within CA265_RS08940at 2232.405 kb on + strand, within CA265_RS08940at 2232.405 kb on + strand, within CA265_RS08940at 2232.407 kb on + strandat 2232.407 kb on + strand, within CA265_RS08940at 2232.407 kb on + strand, within CA265_RS08940at 2232.432 kb on - strand, within CA265_RS08940at 2232.454 kb on - strand, within CA265_RS08940at 2232.476 kb on - strand, within CA265_RS08940at 2232.507 kb on - strandat 2232.562 kb on + strand, within CA265_RS08945at 2232.594 kb on - strand, within CA265_RS08945at 2232.614 kb on - strand, within CA265_RS08945at 2232.617 kb on + strand, within CA265_RS08945at 2232.654 kb on + strand, within CA265_RS08945at 2232.677 kb on + strand, within CA265_RS08945at 2232.689 kb on + strand, within CA265_RS08945at 2232.701 kb on + strand, within CA265_RS08945at 2232.707 kb on - strand, within CA265_RS08945at 2232.707 kb on - strand, within CA265_RS08945at 2232.765 kb on - strandat 2232.930 kb on + strandat 2232.945 kb on + strandat 2232.945 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.12 mg/ml
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2,231,534 + -1.3
2,231,539 + -1.2
2,231,565 - -0.3
2,231,565 - -1.0
2,231,583 - -0.7
2,231,684 - -0.6
2,231,692 + +0.7
2,231,723 + -0.1
2,231,728 - +0.2
2,231,728 - +0.1
2,231,809 - CA265_RS08935 0.31 +0.5
2,231,913 + CA265_RS08935 0.62 -0.8
2,231,913 + CA265_RS08935 0.62 -0.3
2,231,913 + CA265_RS08935 0.62 -1.0
2,231,953 + CA265_RS08935 0.74 -0.2
2,231,953 + CA265_RS08935 0.74 +0.4
2,231,954 - CA265_RS08935 0.74 +0.3
2,231,995 + CA265_RS08935 0.87 +0.0
2,231,996 - CA265_RS08935 0.87 -0.7
2,231,996 - CA265_RS08935 0.87 -0.6
2,232,173 + CA265_RS08940 0.17 +0.2
2,232,174 - CA265_RS08940 0.17 +0.3
2,232,175 + CA265_RS08940 0.18 -0.3
2,232,196 + CA265_RS08940 0.23 -0.7
2,232,196 + CA265_RS08940 0.23 -0.3
2,232,196 + CA265_RS08940 0.23 -0.3
2,232,196 + CA265_RS08940 0.23 -0.5
2,232,197 - CA265_RS08940 0.23 +0.3
2,232,197 - CA265_RS08940 0.23 +0.4
2,232,197 - CA265_RS08940 0.23 +0.3
2,232,197 - CA265_RS08940 0.23 -0.7
2,232,199 + CA265_RS08940 0.23 -0.2
2,232,199 + CA265_RS08940 0.23 -0.2
2,232,220 - CA265_RS08940 0.28 +0.3
2,232,222 + CA265_RS08940 0.29 +0.5
2,232,304 + CA265_RS08940 0.48 -1.8
2,232,305 - CA265_RS08940 0.49 -0.3
2,232,405 + CA265_RS08940 0.72 -0.1
2,232,405 + CA265_RS08940 0.72 +0.2
2,232,407 + +1.1
2,232,407 + CA265_RS08940 0.73 -0.4
2,232,407 + CA265_RS08940 0.73 +0.1
2,232,432 - CA265_RS08940 0.79 -0.8
2,232,454 - CA265_RS08940 0.84 +0.2
2,232,476 - CA265_RS08940 0.89 +1.3
2,232,507 - +0.6
2,232,562 + CA265_RS08945 0.15 -0.2
2,232,594 - CA265_RS08945 0.27 -1.8
2,232,614 - CA265_RS08945 0.34 -0.2
2,232,617 + CA265_RS08945 0.36 -1.1
2,232,654 + CA265_RS08945 0.50 +0.5
2,232,677 + CA265_RS08945 0.59 -0.3
2,232,689 + CA265_RS08945 0.63 -0.6
2,232,701 + CA265_RS08945 0.68 -0.4
2,232,707 - CA265_RS08945 0.70 -1.5
2,232,707 - CA265_RS08945 0.70 -1.2
2,232,765 - +0.0
2,232,930 + -0.9
2,232,945 + +0.4
2,232,945 + -0.3

Or see this region's nucleotide sequence