Experiment: R2A with D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04390 and CA265_RS04395 overlap by 8 nucleotides CA265_RS04395 and CA265_RS04400 are separated by 109 nucleotides CA265_RS04400 and CA265_RS04405 are separated by 176 nucleotides
CA265_RS04390: CA265_RS04390 - histidine phosphatase family protein, at 1,054,277 to 1,054,774
_RS04390
CA265_RS04395: CA265_RS04395 - heptosyltransferase, at 1,054,767 to 1,055,798
_RS04395
CA265_RS04400: CA265_RS04400 - hypothetical protein, at 1,055,908 to 1,056,510
_RS04400
CA265_RS04405: CA265_RS04405 - DNA polymerase/3'-5' exonuclease PolX, at 1,056,687 to 1,058,363
_RS04405
Position (kb)
1054
1055
1056 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1053.774 kb on + strand at 1053.825 kb on - strand at 1053.869 kb on - strand at 1053.871 kb on - strand at 1053.905 kb on + strand at 1053.911 kb on + strand at 1053.911 kb on + strand at 1053.912 kb on - strand at 1053.925 kb on + strand at 1053.965 kb on + strand at 1054.069 kb on - strand at 1054.122 kb on - strand at 1054.132 kb on + strand at 1054.132 kb on + strand at 1054.133 kb on - strand at 1054.137 kb on + strand at 1054.201 kb on + strand at 1054.202 kb on - strand at 1054.269 kb on - strand at 1054.334 kb on - strand, within CA265_RS04390 at 1054.399 kb on - strand, within CA265_RS04390 at 1054.415 kb on + strand, within CA265_RS04390 at 1054.415 kb on + strand, within CA265_RS04390 at 1054.559 kb on - strand, within CA265_RS04390 at 1054.617 kb on - strand, within CA265_RS04390 at 1054.617 kb on - strand, within CA265_RS04390 at 1054.761 kb on - strand at 1054.798 kb on - strand at 1054.814 kb on + strand at 1054.818 kb on + strand at 1054.824 kb on + strand at 1054.942 kb on + strand, within CA265_RS04395 at 1055.018 kb on + strand, within CA265_RS04395 at 1055.023 kb on - strand, within CA265_RS04395 at 1055.027 kb on + strand, within CA265_RS04395 at 1055.028 kb on + strand, within CA265_RS04395 at 1055.028 kb on + strand, within CA265_RS04395 at 1055.029 kb on - strand, within CA265_RS04395 at 1055.253 kb on - strand, within CA265_RS04395 at 1055.253 kb on - strand, within CA265_RS04395 at 1055.276 kb on - strand, within CA265_RS04395 at 1055.348 kb on + strand, within CA265_RS04395 at 1055.349 kb on - strand, within CA265_RS04395 at 1055.460 kb on + strand, within CA265_RS04395 at 1055.460 kb on + strand, within CA265_RS04395 at 1055.461 kb on - strand, within CA265_RS04395 at 1055.582 kb on - strand, within CA265_RS04395 at 1055.606 kb on + strand, within CA265_RS04395 at 1055.645 kb on + strand, within CA265_RS04395 at 1055.769 kb on + strand at 1055.848 kb on + strand at 1055.848 kb on + strand at 1055.870 kb on + strand at 1055.903 kb on + strand at 1055.906 kb on + strand at 1055.907 kb on - strand at 1055.909 kb on + strand at 1055.910 kb on - strand at 1055.916 kb on + strand at 1055.919 kb on + strand at 1055.921 kb on + strand at 1055.922 kb on - strand at 1055.968 kb on - strand at 1055.971 kb on + strand, within CA265_RS04400 at 1056.052 kb on + strand, within CA265_RS04400 at 1056.055 kb on + strand, within CA265_RS04400 at 1056.056 kb on - strand, within CA265_RS04400 at 1056.126 kb on + strand, within CA265_RS04400 at 1056.126 kb on + strand, within CA265_RS04400 at 1056.150 kb on + strand, within CA265_RS04400 at 1056.163 kb on + strand, within CA265_RS04400 at 1056.240 kb on - strand, within CA265_RS04400 at 1056.290 kb on - strand, within CA265_RS04400 at 1056.336 kb on + strand, within CA265_RS04400 at 1056.340 kb on + strand, within CA265_RS04400 at 1056.341 kb on - strand, within CA265_RS04400 at 1056.341 kb on - strand, within CA265_RS04400 at 1056.462 kb on - strand at 1056.468 kb on + strand at 1056.522 kb on - strand at 1056.629 kb on + strand at 1056.698 kb on + strand at 1056.698 kb on + strand at 1056.698 kb on + strand at 1056.709 kb on + strand at 1056.718 kb on - strand at 1056.759 kb on + strand at 1056.780 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.12 mg/ml remove 1,053,774 + -0.6 1,053,825 - -1.3 1,053,869 - -1.1 1,053,871 - +1.1 1,053,905 + -1.1 1,053,911 + -0.9 1,053,911 + -0.3 1,053,912 - +0.2 1,053,925 + -0.4 1,053,965 + +0.0 1,054,069 - +0.4 1,054,122 - -2.6 1,054,132 + +0.6 1,054,132 + -0.3 1,054,133 - -1.4 1,054,137 + +0.7 1,054,201 + +0.0 1,054,202 - +0.3 1,054,269 - +0.9 1,054,334 - CA265_RS04390 0.11 +0.9 1,054,399 - CA265_RS04390 0.24 -0.4 1,054,415 + CA265_RS04390 0.28 +0.1 1,054,415 + CA265_RS04390 0.28 +0.7 1,054,559 - CA265_RS04390 0.57 +2.0 1,054,617 - CA265_RS04390 0.68 +0.5 1,054,617 - CA265_RS04390 0.68 +0.4 1,054,761 - -0.5 1,054,798 - +1.9 1,054,814 + +1.2 1,054,818 + +0.6 1,054,824 + +2.3 1,054,942 + CA265_RS04395 0.17 +1.9 1,055,018 + CA265_RS04395 0.24 +1.3 1,055,023 - CA265_RS04395 0.25 +1.3 1,055,027 + CA265_RS04395 0.25 +1.6 1,055,028 + CA265_RS04395 0.25 +1.5 1,055,028 + CA265_RS04395 0.25 +1.5 1,055,029 - CA265_RS04395 0.25 +0.9 1,055,253 - CA265_RS04395 0.47 +1.0 1,055,253 - CA265_RS04395 0.47 +2.5 1,055,276 - CA265_RS04395 0.49 +2.3 1,055,348 + CA265_RS04395 0.56 +1.4 1,055,349 - CA265_RS04395 0.56 +1.6 1,055,460 + CA265_RS04395 0.67 +1.7 1,055,460 + CA265_RS04395 0.67 +0.9 1,055,461 - CA265_RS04395 0.67 +1.5 1,055,582 - CA265_RS04395 0.79 +0.9 1,055,606 + CA265_RS04395 0.81 +0.7 1,055,645 + CA265_RS04395 0.85 +0.4 1,055,769 + +1.1 1,055,848 + +0.6 1,055,848 + -0.5 1,055,870 + +1.6 1,055,903 + -0.1 1,055,906 + +0.1 1,055,907 - -0.4 1,055,909 + +0.0 1,055,910 - +0.2 1,055,916 + +1.6 1,055,919 + +0.9 1,055,921 + +0.9 1,055,922 - +1.3 1,055,968 - +1.6 1,055,971 + CA265_RS04400 0.10 +1.8 1,056,052 + CA265_RS04400 0.24 +1.4 1,056,055 + CA265_RS04400 0.24 +1.5 1,056,056 - CA265_RS04400 0.25 +0.8 1,056,126 + CA265_RS04400 0.36 +1.7 1,056,126 + CA265_RS04400 0.36 +1.0 1,056,150 + CA265_RS04400 0.40 +1.5 1,056,163 + CA265_RS04400 0.42 +0.6 1,056,240 - CA265_RS04400 0.55 +1.4 1,056,290 - CA265_RS04400 0.63 +2.1 1,056,336 + CA265_RS04400 0.71 +1.9 1,056,340 + CA265_RS04400 0.72 +1.4 1,056,341 - CA265_RS04400 0.72 +0.1 1,056,341 - CA265_RS04400 0.72 +2.3 1,056,462 - +1.7 1,056,468 + +1.5 1,056,522 - -0.0 1,056,629 + -0.4 1,056,698 + -0.4 1,056,698 + -0.2 1,056,698 + -0.6 1,056,709 + -0.3 1,056,718 - +0.1 1,056,759 + -1.0 1,056,780 + -0.1
Or see this region's nucleotide sequence