Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2145 and Shewana3_2146 are separated by 254 nucleotides Shewana3_2146 and Shewana3_2147 are separated by 25 nucleotides Shewana3_2147 and Shewana3_2148 are separated by 70 nucleotides Shewana3_2148 and Shewana3_2149 are separated by 20 nucleotides
Shewana3_2145: Shewana3_2145 - aminotransferase AlaT (RefSeq), at 2,569,796 to 2,571,010
_2145
Shewana3_2146: Shewana3_2146 - hypothetical protein (RefSeq), at 2,571,265 to 2,571,852
_2146
Shewana3_2147: Shewana3_2147 - deoxyguanosinetriphosphate triphosphohydrolase-like protein (RefSeq), at 2,571,878 to 2,573,218
_2147
Shewana3_2148: Shewana3_2148 - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (RefSeq), at 2,573,289 to 2,573,930
_2148
Shewana3_2149: Shewana3_2149 - phosphogluconate dehydratase (RefSeq), at 2,573,951 to 2,575,777
_2149
Position (kb)
2571
2572
2573
2574 Strain fitness (log2 ratio)
-2
-1
0
1 at 2570.915 kb on - strand at 2571.042 kb on + strand at 2571.564 kb on + strand, within Shewana3_2146 at 2571.986 kb on + strand at 2572.052 kb on + strand, within Shewana3_2147 at 2572.423 kb on + strand, within Shewana3_2147 at 2572.664 kb on + strand, within Shewana3_2147 at 2572.779 kb on + strand, within Shewana3_2147 at 2572.996 kb on - strand, within Shewana3_2147 at 2573.153 kb on + strand at 2574.181 kb on - strand, within Shewana3_2149
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,570,915 - -0.5 2,571,042 + -0.3 2,571,564 + Shewana3_2146 0.51 -2.0 2,571,986 + +0.0 2,572,052 + Shewana3_2147 0.13 -0.3 2,572,423 + Shewana3_2147 0.41 -0.5 2,572,664 + Shewana3_2147 0.59 -1.7 2,572,779 + Shewana3_2147 0.67 -0.7 2,572,996 - Shewana3_2147 0.83 -1.0 2,573,153 + +0.4 2,574,181 - Shewana3_2149 0.13 -0.7
Or see this region's nucleotide sequence